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Antagonistic Potential against Pathogenic Microorganisms and Hydrogen Peroxide Production of Indigenous lactobacilli Isolated from Vagina of Chinese Pregnant Women

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Biomedical and Environmental Sciences 2008年第5期21卷 365-371页

作者： HENG-YI XU WAN-HONG TIAN CUI-XIANG WAN LI-JUN JIA LAN-YIN WANG JING YUAN CHUN-MEI LIU MING ZENG HUA WEI State Key Laboratory of Food Science and Technology Jiangxi-OAl Joint Research Institute Nanchang University Nanchang 330047 Jiangxi China National Institute for the Control of Pharmaceutical and Biological Products Beijing 100050 China Beijing Tiantan Hospital Beijing 100050 China Instiute of Disease Control and Prevention Academy of Military Medical Sciences Beijing 100071 China

Objective To investigate the indigenous lactobacilli from the vagina of pregnant women and to screen the isolates with antagonistic potential against pathogenic microorganisms. Methods The strains were isolated from pregnant women＇s vagina and identified using the API50CH system. The ability of the isolates to produce hydrogen peroxide was analyzed semi-quantitatively using the TMB-HRP-MRS agar. The antagonistic effects of the isolates on pathogenic microorganisms were determined with a double layer agar plate. Results One hundred and three lactobacilli strains were isolated from 60 samples of vaginal secretion from healthy pregnant women. Among them, 78 strains could produce hydrogen peroxide, in which 68%, 80%, 80%, and 88% had antagonistic effects against Candida albicans CMCC98001, Staphylococcus aureus CMCC26003, Escherichia coli CMCC44113, and Pseudomonas aeruginosa CMCC10110, respectively. Conclusion The recovery of hydrogen peroxide-producing lactobacilli decreases with the increasing pregnant age and time. The most commonly isolated species from vagina of Chinese pregnant women are Lactobacillus acidophilus and Lactobacillus crispatus. Most of L. acidophilus and L. crispatus produce a high H2O2 level.

关键词： lactobacilli H2O2 Pregnant women Antagonistic Pathogen

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Screening for probiotic characters in lactobacilli isolated from chickens revealed the intra-species diversity of Lactobacillus brevis

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Animal Nutrition 2021年第1期7卷 119-126页

作者： Nasrin Noohi Moslem Papizadeh Mahdi Rohani Malihe Talebi Mohammad R.Pourshafie Research Center for Conservation of Cultural Relics Research Center of Iranian Cultural Heritage and Tourism OrganizationTehranIran Department of Microbiology Pasteur Institute of IranTehranIran Department of Microbiology School of MedicineIran University of Medical SciencesTehranIran

Considering the importance of the poultry industry and the increasing interest in alternative growth promoters,probiotics are considered as a potential candidate for use in the poultry industry.In this study,Lactobacillus species were isolated from 21 rectal swabs of 11 healthy 6-day-old and 10 healthy 21-day-old chickens and their fecal and feed samples.The isolates were characterized and their probiotic characteristics,including resistance to gastric acid and bile salts,biofilm formation and adherence to epithelium or mucus,amylase and protease activity and production of inhibitory compounds,were assessed.From 31 acid and bile resistant lactobacilli,only 2 Lactobacillus brevis and 1 Lactobacillus reuteri strains showed significant probiotic properties.These isolates indicated detectable attachment to Caco-2 cells and significant antibacterial activities against Gram-positive and Gram-negative pathogens.Additionally,phenotypic and genotypic diversity of lactobacilli isolates were studied by Phene Plate(PhP)system(PhP-LB)and random amplified polymorphic DNA(RAPD)-PCR,respectively.PhP-LB results of 24 L.brevis isolates showed a high phenotypic variation among the isolates.In comparison,results of RAPD-PCR highlighted a low diversity.Therefore,it seems that combination of the 2 techniques(PhP and RAPD-PCR)could result in a significant discriminatory power than each of them used alone.

关键词： Probiotic lactobacilli Poultry Feed Fecal

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Gut Bacterial and lactobacilli Communities of Weaning Piglets in Response to Mannan Oligosaccharide and Sugar Beet Pulp In vitro Fermentation

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Journal of Integrative Agriculture 2012年第1期11卷 122-133页

作者： HANG Su-qin ZHU Wei-yun Laboratory of Gastrointestinal Microbiology Nanjing Agricultural UniversityNanjing 210095P.R.China

Microbiota in the gastrointestinal tract （GIT） of piglets during weaning transition can experience a sharp change which could result in growth reduction and diarrhea of weaned piglets. Dietary manipulations can play an important role in attenuating such changes caused by weaning stress. Therefore, ileal and colonic contents of weaned piglets were used as inocula, mannan oligosaccharide （MOS） or sugar beet pulp （SBP） was supplied as single energy sources to investigate effects of MOS or SBP on the shifts of gastro-intestinal microflora and lactobacilli populations. The universal bacteria- and lactobacilli-specific PCR/denaturing gradient gel electrophoresis （DGGE）, cloning and sequencing techniques were used. DGGE profiles of the universal bacteria showed that great changes were found in the position, numbers and intensity of dominant bands after fermentation. The similarity of bacterial community between ileum and colon was increased to 85-97% by MOS or SBP treatment after fermentation from the similarity with 20% before fermentation. MOS treatment significantly increased the bacterial diversity and band number in both ileal and colonic fermentation （P〈0.05）. SBP treatment significantly increased the bacterial diversity and band number in colon （P〈0.05）. It implies that some species were enriched by the addition of MOS or SBP to increase the similarity and diversity of bacterial community in weaned piglets. Five specific bands appearing in MOS or SBP treatment group after fermentation were cloned and sequenced, the changes of species related to Prevotella and Ruminococcus were observed. Two bands related to uncultured bacterium with 98% similarity were detected by MOS or SBP treatment. However, there were no effects on the similarity, diversity index and lactobacilli species revealed by MOS or SBP treatment. These results imply that MOS or SBP could have beneficial effects on the weaning piglets by stablizing microbiota in the GIT microflora.

关键词： mannan oligosaccharide （MOS） sugar beet pulp （SBP） bacterial communities lactobacilli weaning piglets

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Characterizing Diversity of lactobacilli Associated with Severe Early Childhood Caries: A Study Protocol

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Advances in Microbiology 2015年第1期5卷 9-20页

作者： Yihong Li Silvia Argimón Catherine N.Schon Prakaimuk Saraithong Page W.Caufield Department of Basic Science and Craniofacial Biology College of DentistryNew York UniversityNew YorkUSA Department of Cariology and Comprehensive Care College of DentistryNew York UniversityNew YorkUSA

lactobacilli have been consistently associated with dental caries for decades;however, knowledge of this group of bacteria in the etiology of the disease is limited to quantitative elucidation. Nowadays, explicit identification of oral Lactobacillus species is possible, despite their taxonomic complexity. Here we describe a combined approach involving both cultivation and genetic methods to ascertain and characterize the diversity and abundance of the Lactobacillus population in the oral cavities of children with severe early childhood caries (S-ECC). Eighty 3- to 6-year-old children (40 S-ECC and 40 caries free) who were seeking dental care at the Pediatric Dental Clinic of Bellevue Hospital in New York City were invited to participate in this study. Clinical data on sociodemographic information and oral health behavior were obtained from the primary caregiver. The data included a detailed dental examination, children’s medical history, and a questionnaire survey. Combined non-stimulated saliva and supragingival plaque samples were collected from each child and cultivated on selective media for quantitative measures of lactobacilli levels. The procedure for Lactobacillus species screening will include the random selection of 50 colonies per plate, ex- traction of DNA from each colony, and genotyping by arbitrarily primed polymerase chain reaction (AP-PCR). Each unique Lactobacillus AP-PCR genotype will be selected for taxonomic assessment by 16S rRNA gene sequencing analysis. Lactobacillus species will be identified by comparing the 16S rRNA sequences with the Ribosomal Database and the Human Oral Microbiome Database. Meanwhile, the same set of clinical samples will be independently subjected to genomic DNA isolation, 16S rRNA amplification with Lactobacillus genus-specific primers, sequencing, and taxonomic identification, both at genus and species levels with a customized pipeline. The distribution and phylogenetic differences of these Lactobacillus species will be compared between children with or without S-E

关键词： lactobacilli Early Childhood Caries Bacterial Diversity 16S rRNA AP-PCR Saliva Dental Plaque

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Probiotic Screening of lactobacilli Isolates from Uttapam Batter Fermented Supplementing with <i>Piper betle</i>L. Leaves

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Advances in Microbiology 2015年第13期5卷 858-870页

作者： Ashwini Kumar Dubey Kadirvelu Jeevaratnam Department of Biochemistry and Molecular Biology Pondicherry University Puducherry India

The in vitro screening of lactobacilli isolates from uttapam batter, fermented supplementing with Piper betle L. leaves, was performed in order to select potent isolates for probiotic use. Their resistance to simulated gastric and intestinal juices as well as their adhesion to epithelial intestinal HCT-15 and vaginal HeLa cell lines were assessed and also evaluated for their immunomodulatory ability in ex vivo condition. Isolates were able to adhere as well as reduce pathogen adhesion to monolayer cell lines’ surfaces and reduced production of pro-inflammatory cytokine TNF-α in LPS treated PBMCs, while enhanced that of anti-inflammatory cytokine IL-10. The isolates exhibited properties of auto-aggregation, co-aggregation, hydrophobicity, bile salt hydrolase activity and strong antimicrobial activity against pathogenic bacteria. Thus, the different lactobacilli isolates displayed potent probiotic and immunomodulatory properties among which AJ7 and AJ82 had a great potential and may have applications in fermented foods as immunomodulatory probiotic additives.

关键词： Acid Tolerance Adhesion Immune Modulation lactobacilli Probiotic

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<i>lactobacilli</i>Enjoyed Fermented Herbs on to the Last Fragment and Regulated Leucocyte Subsets and Anti-Oxidative Activity

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Open Journal of Rheumatology and Autoimmune Diseases 2017年第1期7卷 30-45页

作者： Nobuo Yamaguchi Yoshiichiroh Mastuba Kazuhiro Okamoto Daisuke Sakamoto Takanao Ueyama Hideo Matsuno Nurmuhamamt Amat Ishikawa Natural Medicinal Products Research Center Ishikawa Japan Department of Rehabilitation Kanazawa Medical University Uchinada-Machi Japan Department of Heart Surgery Kanazawa Medical University Himi Municipal Hospital Himi Japan Department of 2nd Internal Medicine Kansai Medical University Osaka Japan Department of Otolaryngology Kanazawa Medical University Uchinada-Machi Japan Traditional Uighur Medicine Department Xinjiang Medical University Urumqi China

Eighty sorts of herbs were fermented by Lactobaccilli (f-ESH). This material was proved by as safe in animal safety experiment. This work was aimed to revise the traditional way of hot water extraction to the fermentation, in order to use up the original material and searching new activity as well. We tried to prove the new activity by fermentation for immune-competent cells in the host that administrated both of original remedy and the new fermented sample. In rodents, immune-compromised host was prepared with cancer chemotherapeutic agent. Our observations showed that the antigen stimulated animal increased the antibody producing cell for heterologous erythrocyte by compromised host by f-ESH. Moreover, by administration of f-ESH to immno-suppressed hosts, these samples regulated lymphocyte in number and functions, macrophage activities, and regulating anti-oxidative activity by phagocytic cells. The anti-oxidative assay was carried out ex-vivo system by peritoneal excaudate cell that we propose as suitable system for evaluating antioxidative assay. With these evidences, the original COF only augmented the level of lymphocytes in number, while f-ESH regulated the ratio of granulocytes and lymphocyte. In clinical study with 20 healthy volunteers, granulocyte and lymphocyte ratio was also resulted as neutral in peripheral white blood cells, 1, 2 and 3 weeks after the administration of f-ESH. We discussed the significance and mechanism of cleving complement components Fb by f-ESH in this text. Fb. The new cleavage of complement was directly evident by immune-electrophoretic method. In conclusion, this modification of food materials introduced new style of food intake resulted in the significant regulation such as antibody producing cells and anti-oxidative activity for phagocytic cells comparing traditional processing by hot water extraction.

关键词： Wild Herbs Fermentation lactobacilli Lymphocyte Granulocyte Ant-Oxidative Activity GABA Complement Alternative Complement Activation

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The use of Lactobacillus as an alternative of antibiotic growth promoters in pigs:A review

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Animal Nutrition 2017年第1期3卷 1-6页

作者： Runjun Dowarah A.K.Verma Neeta Agarwal CAFT in Animal Nutrition Indian Veterinary Research Institute

Antibiotics, often supplemented in feed, used as a growth promoter, may cause their residual effect in animal produce and also trigger antibiotic resistance in bacteria, which is of serious concern among swine farming entrepreneurs. As an alternative, supplementing probiotics gained interest in recent years.Lactobacillus being the most commonly used probiotic agent improves growth performance, feed conversion efficiency, nutrient utilization, intestinal microbiota, gut health and regulates immune system in pigs. The characteristics of Lactobacillus spp. and their probiotic effects in swine production are reviewed here under.

关键词： Diarrhea score Gut microbiota lactobacilli Probiotics

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Lactobacillus isolates from healthy volunteers exert immunomodulatory effects on activated peripheral blood mononuclear cells

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The Journal of Biomedical Research 2013年第2期27卷 116-126页

作者： Keyi Sun Chao Xie Donghua Xu Xiaofan Yang James Tang Xiaohui Ji Department of Microbiology and Immunology Nanjing Medical University Cultech Limited Unit 2 Christchurch Road Baglan Industrial Park

As probiotics in the gut, lactobacilli are believed to play important roles in the development and maintenance of both the mucosal and systemic immune system of the host. This study was aimed to investigate the immunomodulatory function of candiate lactobacilli on T cells. lactobacilli were isolated from healthy human feces and the microbiological characteristics were identified by API 50 CHL and randomly amplified polymorphic DNA （RAPD） assays. Anti-CD3 antibody activated peripheral blood mononuclear cells （PBMCs） were treated by viable, heat-killed lactobacilli and genomic DNA of lactobacilli, and cytokine profiles were tested by ELISA. Isolated lactobacilli C44 and C48 were identified as L. acidophilus and L. paracacei, which have properties of acid and bile tolerance and inhibitor effects on pathogens. Viable and heat-killed C44 and C48 induced low levels of proinflammatory cytokines （TNF-a, IL-6 and IL-8） and high levels of IFN-y and IL-12p70 in PBMCs. In anti- CD3 antibody activated PBMCs, viable and heat-killed C44 increased Th2 cytokine levels （IL-5, IL-6 and IL-10）, and simultaneously enhanced Thl responses by inducing IFN-y and IL-12p70 production. Different from that of lactabacillus strains, their genomic DNA induced low levels of IL-12p70, IFN-y and proinflammatory cytokines in PBMCs with or without anti-CD3 antibody activation. These results provided in vitro evidence that the genomic DNA of strains of C44 and C48, especially C44, induced weaker inflammation, and may be potentially applied for treating allergic diseases.

关键词： lactobacilli probiotics Thl Th2 cytokine immuno-modulation

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Probiotics for Antibiotic-Associated Diarrhea: What, When, and How Long?

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International Journal of Clinical Medicine 2022年第12期13卷 571-583页

作者： John R. Ferguson Karen Taylor New Paltz USA Biocodex Bedminster USA

Probiotics have been formally defined as “live microorganisms that confer a health benefit on the host when administered in adequate amounts.” Although a range of applications has been explored for probiotics, their utility in antibiotic-associated diarrhea (AAD) is both biologically plausible and supported by abundant clinical evidence. However, the strength of evidence underlying the efficacy of specific strains and formulations for AAD varies widely. This review leverages recent meta-analyses and systematic reviews to clarify some outstanding issues on the utility of probiotics for AAD, including which strains have evidence for efficacy in AAD, what doses have been demonstrated to be effective, and the optimal duration of probiotic therapy, and provides practical guidance on how to select an appropriate product. Some trends emerged in this analysis of recent meta-analyses and systematic reviews, including: 1) Certain probiotics, such as Saccharomyces boulardii and some lactobacilli-containing products, are consistently found to be effective for the management of AAD;2) Dosing thresholds for efficacy exist that must be achieved through the administration of probiotics that reliably contain the labeled amounts of probiotic constituents;3) Most effective probiotics are initiated at the same time as antibiotic therapy and continued for between 1 and 3 weeks after the cessation of therapy. These data suggest that attention must be paid to species, dose, and duration when selecting an appropriate product for patients initiating antibiotic therapy;further considerations may include the antibiotic used and the patient’s baseline risk for AAD.

关键词： Probiotics AAD Saccharomyces boulardii lactobacilli

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Antioxidant Properties of Fermented Camel Milk Prepared Using Different Microbial Cultures

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Food and Nutrition Sciences 2022年第11期13卷 861-877页

作者： Nagwa Hussein Ismail Abou-Soliman Mahmoud Ibrahim El-Sayed Sameh Awad Department of Animal Breeding Animal and Poultry Production Division Desert Research Centre Cairo Egypt Department of Dairy Technology Research Food Technology Research Institute Agricultural Research Center Giza Egypt Department of Dairy Science and Technology Faculty of Agriculture Alexandria University Alexandria Egypt

This study investigated the effect of using different combinations of commercial starter culture and lactobacilli strains on the antioxidant properties of fermented camel milk for 14 days. The bacterial strains included Lb. casei subsp. casei B-1922, Lb. paracasei subsp. paracasei B-4560, Lb. rhamnosus B-442 and Lb. rhamnosus B-1445. The antioxidant activity of fermented milk was estimated using DPPH radical scavenging activity, ferrous ion chelating activity (FCA) and ferric reducing power assays. The total phenolic content (TPC), titratable acidity, proteolysis degree and sensory acceptability of samples were also evaluated. The results showed that all the studied parameters were affected by both the type of starter culture and storage. Samples containing the commercial starter and Lb. rhamnosus B-1445 had the highest DPPH radical scavenging activity and TPC throughout storage. Fermented milks prepared using the commercial starter and Lb. rhamnosus B-442 or Lb. paracasei retained their high FCA and reducing power throughout storage compared to the other samples. Fermented milk containing the commercial starter and Lb. casei showed the lowest antioxidant activity. The DPPH scavenging activity for all fermented milks decreased sharply during storage, while the change in FCA, reducing power and TPC differed among the samples during storage. The highest acidity was observed in the samples containing the commercial starter and Lb. paracasei, while fermented milk prepared using commercial starter and Lb. rhamnosus B-1445 had the lowest acidity. Samples containing the commercial starter and Lb. casei had the greatest proteolysis during the first week of storage, while samples containing the commercial starter and Lb. rhamnosus B-1445 had the highest proteolysis on day 14. Fermented milks with commercial starter and Lb. rhamnosus B-1445 or Lb. paracasei were the most acceptable products, while samples containing the commercial starter and Lb. casei were less acceptable.

关键词： Antioxidant Activity Fermented Camel Milk lactobacilli

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