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American Journal of Plant Sciences 2014年第19期5卷 2869-2877页

作者： Kartini Ahmad Siow-Ping Tan Mohd Aspollah Sukari Abdul Manaf Ali Mohd Azlan Nafiah Department of Chemistry Faculty Science and Mathematics University Sultan Idris Tanjong Malim Malaysia Department of Chemistry Faculty Science University Putra Malaysia Serdang Malaysia School of Biotechnology Faculty of Agriculture Biotechnology & Food Science University Sultan Zainal Abidin Kuala Terengganu Malaysia

Murraya koenigii (L.) (Rutaceae) is an indigenous medicinally important herb of Indian origin and now is widely distributed throughout southern Asia. The stem bark, leaves and roots of Malayan Murraya koenigii were selected for phytochemical investigation. Eight carbazole alkaloids was isolated and identified using spectroscopic methods including NMR, IR, UV, MS spectra data. Crude extract and isolated compounds from the roots of this plant were screened for cytotoxic activity and antitumor promoting activity. All crude extracts of the roots including the isolated compounds, mahanimbine, mahanine and murrayafoline-A exhibited significant cytotoxic activity against CEM-SS cell line with IC50 3mg/mL. Girinimbine inhibited EBV-activation in the antitumor promoting assay.

关键词： Carbazole Rutaceae Murraya koenigii NMR Cytotoxic

Green Process for Impregnation of Silver Nanoparticles into Microcrystalline Cellulose and Their Antimicrobial Bionanocomposite Films

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Journal of Biomaterials and Nanobiotechnology 2012年第3期3卷 371-376页

作者： Singaravelu Vivekanandhan Laura Christensen Manjusri Misra Amar Kumar Mohanty Bioproducts Discovery and Development Centre Department of Plant Agriculture Crop Science Building University of Guelph Guelph N1G 2W1 Ontario Canada. School of Engineering Thornborough Building University of Guelph Guelph N1G 2W1 Ontario Canada

A novel greener method to impregnate silver nanoparticles (AgNPs) into microcrystalline cellulose (MCC) by curry leaf (Murraya koenigii) extract mediated biological process is presented. The active reduction of silver ions by curry leaf extract was explored for the in situ impregnation AgNPs into MCC. Transmission electron microscopy (TEM) analyses of MCC coated with AgNPs showed the formation of silver particle sizes in the range of 10-25 nm and have a spherical shape. Further the, EDS analysis of MCC/Ag nanocomposite confirms the formation of Ag structure on microcrystalline cellulose. Solvent casting of poly(lactic-acid) was used to produce composite films containing silver impregnated MCC aiming for antimicrobial applications.

关键词： Silver Nanoparticles Impregnation Bioreduction Murraya koenigii Leaf Extract

Antibacterial compounds from Rutaceae with activities against Flavobacterium columnare and Streptococcus iniae

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Journal of Agricultural Chemistry and Environment 2013年第4期2卷 90-100页

作者： Kumudini M. Meepagala Kevin K. Schrader Charles L. Burandt University of Mississippi National Center for Natural Products Research University MS USA USDA-ARS Natural Products Utilization Research Unit University MS USA

From the ethyl acetate extract of Murraya koenegii (Rutaceae) leaves, isomahanine (1) and mahanine (2) were isolated that showed antibacterial activity towards Flavobacterium columnare and Streptococcus iniae which caused columnaris disease and streptococcosis respectively. Isomahanine was found to have the strongest activity against F. columnare (isolate ALM-00-173) and S. iniae (isolate LA94-426) based on 24-h 50% inhibition concentration (IC50) and minimum inhibition concentration (MIC). Although compound (7), a nicotinamide isolated from Amyris texana had the lowest MIC (2.8 ± 0 mg/L) of any of the test compounds against F. columnare, the 24-h IC50 of 14.8 ± 0.6 mg/L was higher than that of isomahanine and subsequently the 24-h IC50 RDC values for (7) were almost a magnitude of order higher than those obtained for isomahanine. Isomahanine also had the strongest activity against S. iniae, with a 24-h IC50 of 1.3 ± 0.1 mg/L and MIC of 3.5 ± 0 mg/L, respectively.

关键词： Murraya koenigii Amyris texana Piper nigrum Columnaris Disease Flavobacterium columnare Streptococcosis Streptococcus iniae Aquaculture

Leafy Vegetables as Potential Pathways to Heavy Metal Hazards

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Journal of Agricultural Chemistry and Environment 2019年第1期8卷 23-32页

作者： C. E. Anarado C. J. O. Anarado M. O. Okeke C. E. Ezeh N. L. Umedum P. C. Okafor Department of Pure and Industrial Chemistry Nnamdi Azikiwe University Awka Nigeria

The effect of anthropogenic activity relating to industrial and economic development has had a detrimental impact on the environment and human health, and hence the need for continued research. Five common African vegetables—Murraya koenigii, Ocimum gratissimum, Amaranthus hybridus, Capsicum annuum and Moringa oleifera were used to study absorption of Lead, Cadmium, Cobalt and Zinc from soils inoculated with metal ions. 0.1 M and 0.5 M solutions of the metal ions were used in the inoculation. Each of the plants was collected in the first instance at 8 weeks, and then at 10 weeks of inoculating. Atomic Absorption spectrophotometer was used to determine the metal ions concentrations absorbed in the plants. Cd2+ was most and Moringa oleifera the least absorbed of the four metal ions, with a highest value of 34.801 ± 0.805 mg/kg occurring in Capsicum annuum. Co2+ was the least absorbed of the four metal ions, Amaranthus hybridus showed highest absorption of Co2+ with mean absorption values of 5.566 ± 0.324 mg/kg and 5.670 ± 0.210 mg/kg for 0.1 M and 0.5 M solution of Co2+ respectively. Ocimum gratissimum absorbed Pb2+ most with the highest mean absorption of 5.290 ± 0.180 mg/kg and 6.354 ± 0.366 mg/kg for 0.1 M and 0.5 M respectively. Absorption increased as the concentration of the inoculant solution increased for all the plants, and decreased on moving from 8 weeks’ to 10 weeks’ for all the plants except Moringa oleifera. This could as a result of Phytovolatilization against the report of Padmavathiamma and Li, 2007 [1] that phytovolatilization occurs in As, Hg and Se. Ocimum gratissimum showed highest absorption with the mean value of 9.334 ± 0.312 mg/kg, when the inoculants concentration increased to 0.5 M, Capsicum annuum showed highest absorption with mean absorption value of 9.916 ± 0.614 mg/kg at 10th week. Also absorption increased as the concentration of the inoculant solution increased, and also on moving from 8 weeks’ to 10 weeks’ for all the plants. From th

关键词： Heavy Metals Ocimum gratissimum Murraya koenigii Capsicum annuum Amaranthus hybridus Moringa oleifera

Iodine Fortification Study of Some Common African Vegetables

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Journal of Agricultural Chemistry and Environment 2019年第3期8卷 172-183页

作者： Chigozie John Onyinye Anarado Charity Ebere Anarado Richard Ifeanyi Areh Nonyelum Ifoh Elijah Okechukwu Eze Ebuka Ikeakor Department of Pure and Industrial Chemistry Nnamdi Azikiwe University Awka Nigeria

Iodine Deficiency Diseases (IDDs) occupy important positions in the health problems of developing countries. Salt Iodisation has been the common approach to solving these problems. However, apart from the problems of lack of compliance by salt manufacturers, and inculturation of the consumers, health conditions aggravated by high salt intake by humans have become increasingly relevant. These problems can be eliminated if the commonly produced and consumed plants are fortified with Iodine. The prospects are in the inclusion of Iodine-containing compounds in the inorganic fertilizers used by farmers. In this study, Potassium Iodide and Potassium Iodate were used as inoculants. Five different concentrations—0.1 M, 0.2 M, 0.3 M, 0.4 M, and 0.5 M of Potassium Iodide and Potassium Iodate solutions were used to inoculate the soils on which the following edible African plants were planted: Murraya koenigii;Ocimum gratissimum;Cucurbita pepo;Solanum nigrum;Amaranthus hybridus and Abelmoschus esclentus, Corchorous olitoruis, Solanum lycopersicum, Zingiber officinale, Telfairia occidentalis, Talinium triangulare, Solanum melongena. Controls were also planted. After 14 days, alkaline dry ash method was used to determine the Iodine concentrations in the plants. The results showed that Murraya koenigii showed the highest absorption of Iodine 6.90 mg/kg at 0.3 M using KI, followed by Amaranthus hybridus 6.40 mg/kg at 0.1 M. Solanum nigrum, Ocimum gratissimum and Zingiber officinale also showed good absorption. Other plants except Murraya koenigii, Ocimum gratissimum, Solanum nigrum and Zingiber officinale showed very low tolerance to KI absorption. The result also showed that Telfairia occidentalis showed the highest absorption of iodine 8.20 mg/kg at 0.2 M of KIO3 followed by Cucurbita pepo 6.40 mg/kg at also 0.2 M of KIO3. Murraya koenigii, Ocimum gratissimum, Solanum nigrum, Zingiber officinale also showed good absorption of KIO3. Some of the plants were not able to tolerate the

关键词： Iodine Deficiency Disease (IDD) Biofortification Iodisation Potassium Io-dide Potassium Iodate: Murraya koenigii Ocimum gratissimum Cucurbita pepo Solanum nigrum Amaranthus hybridus Abelmoschus esclentus Corchorous olitorius Solanum lycopersicum Zingiber officinale Telfairia occidentalis Talinium triangulare Solanum melongena

An Herbal Formula LI85008F Inhibits Lipogenesis in 3T3-L1 Adipocytes

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Food and Nutrition Sciences 2011年第8期2卷 809-817页

作者： Krishanu Sengupta Trimurtulu Golakoti Venkateswara Rao Chirravuri Ajit Kumar Marasetti 不详

The present study demonstrates a novel herbal formulation LI85008F inhibiting adipocyte differentiation and potentiates lipolysis in 3T3-L1 mouse adipocytes. LI85008F is formulated by combining extracts of three Indian herbs Moringa oleifera, Murraya koenigii and Curcuma longa. Oil red O staining of 3T3-L1 adipocytes reveals that LI85008F is a synergistic formulation that inhibits adipocyte differentiation in a dose dependent manner and concurrently down regulates the key adipogenic transcription factors Peroxisome Proliferator-Activated Receptor gamma (PPAR) and CCAAT/enhancer binding protein α (C/EBP). LI85008F confers significant reductions in intracellular triglyceride content in a dose dependent manner. Evidence suggests that LI85008F antagonizes PPAR through Ser112 phosphorylation via MAPK/ERK activation. Immunoblot analyses reveal that LI85008F treatment also down regulates the protein expressions of key PPAR responsive gene products such as Adipocyte differentiation related protein (ADRP), CD36, Adipocyte specific binding protein 2 (aP2) and perilipin. In differentiated adipocytes culture, LI85008F treatment results in significantly (p = 0.0169) increased lipolysis as measured by the release of glycerol. LI85008F does not exhibit cytotoxic effect on adipocytes. Taken together, the results suggest that LI85008F inhibits lipogenesis in adipocytes and concurrently antagonizes PPAR? and other lipogenic factors and in addition, potentiates triglyceride mobilization from the fat cells or enhances lipolysis.

关键词： Adipocytes Adipogenesis Curcuma longa LI85008F Moringa Oleifera Murraya koenigii