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Expression of visual system homeobox 1 in human keratoconus

International Journal of Ophthalmology(English edition) 2019年第2期12卷 201-206页

作者： Ya-Ni Wang Xian-Ning Liu Xiao-Dong Wang Yong Yin Yan Chen Xiang-Hua Xiao Kun Xu Xiu-Ping Zhu Xi’an No.1 Hospital Shaanxi Institute of Ophthalmology Shaanxi Key Laboratory of Ophthalmology Clinical Research Center for Ophthalmology Diseases of Shaanxi Province First Affiliated Hospital of Northwestern University

AIM: To investigate the expression of visual system homeobox 1(VSX1) and myofibroblast marker alpha smooth muscle actin(α-SMA) in keratoconus(KC). METHODS: Thirty corneal tissue were collected from KC patients after corneal transplantation and 15 normal donor corneas were obtained. All corneal tissues divided into 4 parts for different detections. Scanning electron microscopy was used to observe the ultrastructure of the specimens. VSX1 and α-SMA localization in cornea tissues was detected using immunofluorescence histochemistry. Reverse transcription-quantitative polymerase chain reaction(RT-qPCR) and Western blot were performed to analyze the expression level of VSX1 and α-SMA. RESULTS: Compared to normal cornea tissue, the collagen fibers in KC stroma were distortional and attenuated and keratocytes were abnormally changed. VSX1 and α-SMA located in the corneal stroma. The mRNA and protein expression level of VSX1 in KC were about 3 times as high as that of normal tissue(Pkeratocytes and involved in the pathogenesis of keratoconus.

关键词： visual system homeobox 1 keratoconus alpha smooth muscle actin keratocytes

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An Association of Vitamins A and E with Hyaluronic and Lactobionic Acids may Prevent Molecular Changes Associated with Keratocyte to Myofibroblast Transition

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Journal of Integrative Medicine（双语） 2021年第2期10卷 20-29页

作者： Martina Cristaldi Gabriella Lupo Melania Olivieri Giorgia Spampinato Carmelina D.Anfuso Dario Rusciano Research Center Sooft Italia SpA c/o Biologic TowerUniversity of Catania95123 CataniaItaly Department of Biomedical and Biotechnological Sciences Biologic TowerUniversity of Catania95123 CataniaItaly Maurizio Bufalini Hospital Onco-hematology serviceCesenaItaly

Inflammatory events in the corneal stroma may activate keratocytes and trigger their transition towards myofibroblasts,which now produce different extracellular matrix(ECM)proteins thus causing corneal *** haze is a frequent side effect after photorefractive keratectomy(PRK)to correct high ***,a preventive treatment with mitomycin-c can be used to limit the occurrence of this ***,mitomycin-c is a toxic drug,not devoid of side effects,which may occasionally involve the corneal ***,we have searched for a less risky,natural way,to prevent keratocytes *** this purpose,we have used as markers of the phenotype switch the proteins lumican(highly expressed by keratocytes and much less by myofibroblasts)and smooth muscle actin(αSMA)(highly expressed by myofibroblasts and poorly found in keratocytes),beside Fibronectin(Fn),the expression of which is also increased by transforming growth factor-beta(TGFβ)*** of human keratocytes with TGFβwas used to induce the protein *** different possible candidates,we have found that vitamins A and E,hyaluronic and lactobionic acids may prevent,either alone,or much better in association,the shift in the ratio between lumican andαSMA and the increased Fn *** conclusion,it could be speculated that topic treatment of the ocular surface with an association of these four compounds could be able to prevent or at least limit the occurrence of post-PRK corneal haze,with the additional advantage of lubrication,hydration and antioxidant defense exerted by these molecules.

关键词： Corneal haze keratocytes Lumican Vitamins Hyaluronic acid Lactobionic acid

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