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Anti-schistosomal activities of Echinops kebericho Mesfin root and Hagenia abyssinica (Bruce) J.F Gmel flower part crude extracts in Swiss albino mice

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Asian Pacific Journal of Tropical Medicine 2018年第10期11卷 570-575页

作者： Yonas Alemu Zeleke Mekonnen Ahmed Zeynudin Moti Yohannes Abdissa Biruksew Sultan Suleman School of Medical Laboratory Sciences Jimma University School of Veterinary Medicine Jimma University School of Pharmacy Jimma University

Objective: To evaluate the in vivo antischistosomal activities of the crude extracts of Echinops kebericho Mesfin(E. kebericho) root and Hagenia abyssinica(Bruce) J.F. Gmel(H. abyssinica) flower. Methods: Mice were infected with(150 ± 10) Schistosoma mansoni cercariae by paddling technique. Crude extracts were administered orally for five consecutive days at doses of 300, 600 and 1 200 mg/kg/day along with 200 mg/kg/day praziquantel and 3% tween 80 given as a control. Results: E. kebericho root extract showed a statistically significant(P kebericho at 1 200 mg/kg/day dose group as compared to infected untreated control group. Conclusions: H. abyssinica and E. kebericho crude extracts show a promising antischistosomal activity.

关键词： Schistosoma mansoni Echinops kebericho Hagenia abyssinica Crude extract Antischistosomal activity Ethiopia

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Micropropagation of kebericho: An Endandered Ethiopian Medicinal Plant

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American Journal of Plant Sciences 2014年第26期5卷 3836-3847页

作者： Begashaw Manahlie Tileye Feyissa Department of Forestry Wondo Genet College of Forestry and Natural Resource Hawassa University Shashemene Ethiopia Institute of Biotechnology Addis Ababa University Addis Ababa Ethiopia

Echinops kebericho, endemic to Ethiopia, is a critically endangered medicinal plant. It is among the most important medicinal plants of the country, valued primarily for its root parts. The commercial harvesting and sale of roots of E. kebericho have threatened local populations. This study aimed to develop micropropagation protocol for E. kebericho using shoot tip explants. The study started with seed germination test using seeds stored for different months. Shoot tips from in vitro germinated seedlings were cultured on shoot initiation MS media supplemented with 0, 0.1, 0.5, 1.0, 1.5 and 2.0 mg/l BAP or KN alone. Explants were cultured on shoot proliferation media fortified with Kinetin, BAP, and TDZ each at 0.0, 0.5, 1.0, 2.5, 3.0 and 5.0 mg/l either alone or in combination with 0.0, 0.1, 0.25 and 0.5 mg/l NAA. For rooting, full, half and 1/3 strength MS media supplemented with IBA and NAA alone each at 0, 0.05, 0.1, 0.5, 1.0, 1.5 and 2.5 mg/l were used. Growth regulator free MS medium was used as control. Study results showed that 100% germination was recorded in fresh seeds and dropped as low as 65.18% and 22.3% for 3 and 5 months seeds respectively. 1.0 mg/l KN and 0.5 mg/l KN + 0.1 mg/l NAA showed maximum shoot proliferation on shoot induction media and shoot multiplication media respectively. Best rooting was obtained on 1/3 MS containing 1.5 mg/l NAA with 8.23 roots and 4.82 cm root length and established under greenhouse with 83% survival.

关键词： Echinops kebericho Micropropagation Shoot Tip Plant Growth Regulator

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