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karmoisin and cardinal ortholog genes participate in the ommochrome synthesis of Nilaparvata lugens (Hemiptera:Delphacidae)

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Insect Science 2019年第1期26卷 35-43页

作者： Shu-Hua Liu Ju Luo Bao-Jun Yang Ai-Ying Wang Jian Tang State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhouChina

Ommochrome is the major source for eye coloration of all insect species so far *** synthetase (PHS)has always been regarded as the terminal step enzyme for ommochrome formation,which is encoded by cardinal or karmoisin genes. Our previous study indicated that the karmoisin ortholog gene (Nl-karmoisin)product in the brown planthopper (BPH)was a monocarboxylate transporter,while not a PHS. Here,based on full-length complementary DNA,the cardinal ortholog gene in BPH (Nl-cardinal)product was predicted to be a haem peroxidase rather than a *** suggest for the first time that neither karmoisin nor cardinal encodes the PHS,but whether PHS participates in BPH eye pigmentation needs further *** RNA interference (RNAi)experiments showed that knockdown Nl-cardinal transcript led the BPH ocelli and compound eye to color change from brown to red,while knockdown Nl-karmoisin only made the ocelli present the red ***,not only the Nl-cardinal transcript, dscd injection (Nl-cardinal targeting double-stranded DNA (dsRNA))also significantly reduced the Nl-karmoisin transcript by 33.7%,while dska (Nl-karmoisin targeting dsRNA) injection did not significantly change the Nl-cardinal *** the above RNAi and quantitative real-time polymerase chain reaction results,we propose that Nl-cardinal plays a more important role in ommochrome synthesis than Nl-karmoisin,and it may be an upstream gene of *** present study suggested that both karmoisin and cardinal ortholog genes play a role in ommochrome synthesis in a hemimetabolous insect.

关键词： cardinal eye color karmoisin Nilaparvata lugens ommochrome phenoxazinone synthetase

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Cloning and Characterization of karmoisin Homologue Gene(Nlka) in Two Brown Planthopper Strains with Different Eye Colors

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Rice science 2016年第2期23卷 104-110页

作者： LIU Shu-hua TANG Jian LUO Ju YANG Bao-jun WANG Ai-ying WU Jin-cai School of Plant Protection Yangzhou University State Key Laboratory of Rice Biology China National Rice Research Institute

The brown planthopper （BPH）, Nilaparvata lugens, is a destructive insect pest of rice throughout Asia. Different from brown-eye color wild type, BPH also has red-eye color mutation phenotype. As a visible genetic marker, the red-eye mutant in BPH is a valuable material. To reveal the eye color mutation mechanism, a karmoisin homologue gene （named as Nlka） was cloned from BPH. And karmoisin is always deemd as a xanthommatin-related gene in other insects, encoding phenoxazinone synthetase （PHS）. Nlka is consisted of 7 exons and encodes a protein with 502 amino acids （NIKA）. NIKA showed high amino acid identities with its insect homologues （48.8%-51.8%）. Nlka transcripts can be detected at all the developmental stages and in all tissues tested, including egg, nymph, adult, body wall, ovary, fat body, midgut and Malpighian tubule. However, no constant In/Del or non-synonymous mutation was observed between the mutant and the wild type strains. Quantitative real-time PCR experiment also showed that Nlka transcript level had no significant differences between them. These results indicated that Nlka is not the target gene causing the red-eye color mutation phenotype of BPH. Through the second structure and motif analysis, the present study also showed that all the proteins deduced from the karmoisin genes in insects may be members of monocarboxylate transporters （MCTs） rather than PHSs.

关键词： Nilaparvata lugens red-eye mutant karmoisin monocarboxylate transporter phenoxazinone synthetase rice gene clone

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