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A microcalorimetry and Spectroscopy Study on the Interaction of BSA with 2,2′-Bipyridine Octylglycinato Palladium(II) Nitrate

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Acta Biochimica et Biophysica Sinica 2003年第10期35卷 886-890页

作者： MANSOORI-TORSHIZI Hassan,ISLAMI-MOGHADDAM Mahbobe,SABOURY Ali Akbar Institute of Biochemistry and Biophysics Department of Chemistry Institute of Biochemistry and Biophysics University of Tehran Tehran 14176-14411 Iran Department of Chemistry University of Sistan & Bluchestan Zahedan 98167-45345 Iran University of Sistan & Bluchestan Zahedan 98167-45345 Iran University of Tehran Tehran 14176-14411 Iran

The interaction of bovine serum albumin (BSA) with a new palladium(II) complex [Pd(bpy)(Oct-Gly)]NO 3 (bpy, 2,2′-bipyridine; Oct-Gly, octyl-glycine) was studied by isothermal titration UV-visible spectrophotometry and microcalorimetry in 30 mmol/L Tris buffer, pH 7.0. There is a set of 18 binding sites for this complex on BSA at 300 and 310 K with positive cooperativity in the binding process. The Hill coefficients at 300 and 310 K are 2.2 and 2.4, respectively. The binding of this palladium complex on BSA is endothermic with mean association binding constant of 21.0 and 16.4 (mmol/L) -1 at 300 and 310 K, respectively. The complex can denature the protein as surfactants. The stability of BSA in the interaction study with the complex is 84 and 58 kJ/mol at 300 and 310 K, respectively. Also, the enthalpy of BSA denaturation due to the interaction with the complex is 842 kJ/mol.

关键词： serum albumin palladium complex isothermal titration microcalorimetry spectrophotometry

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First Assessment of the Thermodynamic Properties for an Aqueous Two-Phase Systems bath on Ionic Liquid

First Assessment of the Thermodynamic Properties for an Aque...

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中国化学会第十九届全国化学热力学和热分析学术会议

作者： Qi Liu Suxu Wang Xiaoxiao Chen Yan Guo Jie Liu Xilian Wei Liaocheng University

Aqueous two-phase system(ATPS) have been widely used as an extremely promising primary separation unit operation in the downstream biological engineering .A conventional ATPS is composed of two structurally different polymers or one salt and a hydrophilic *** recent years,many new ATPS based on surfactants or ionic solution have been *** new ATPS have been successfully applied in the extraction of biological analysis and have opened a new vista for purification and isolation of *** a later study,a new kind of ATPS’s formed by different ILs and anionic or cationic surfactants in aqueous media were reported by our research *** can be used to separate and purify different biomaterials and to retain the activity of the separated materials as much as *** this basis,we choose a ternary system composed of an ionic liquid [Bmim]BF,Sodium Alkylbenzene Sulfonate(SDBS) and *** phase diagram was drawn based on experimental data at 298.15 *** got a narrow aqueous two-phase region near the side of water-ionic liquid.A series of samples with different compositions around the phase diagram were *** enthalpy change of these systems after injecting triply distilled water into these samples has been studied by using isothermal titration microcalorimetry(ITC).It is found that with increasing the water content there are weak endothermic and exothermic processes appearing,separately,which matches perfectly with the location of the formation and disappearance of the aqueous two-phase in the ternary phase *** suggests the ITC is a feasible and effective method for the thermodynamic study of aqueous two-phase systems(ATPS) and was first *** experimental results show that the formation of the aqueous two-phase system is endothermic(ΔH>0),but the process of disappearing is exothermic(ΔHisothermal constant pressure,we can

关键词： Ternary phase diagram Aqueous two-phase system Sodium dodecylbenzene Sulfonate isothermal titration microcalorimetry

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Thermodynamic Study of the Aqueous Two-Phase Systems of 1-Butyl-3-Methylimidazolium Tetrafluoroborate and Sodium Dodecylbenzenesulfonate

Thermodynamic Study of the Aqueous Two-Phase Systems of 1-Bu...

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中国化学会第十六届胶体与界面化学会议

作者： Guo Yan Chen Xiao-xiao Lv Dong-mei Liu Jie Wei Xi-lian Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology College of Chemistry and Chemical EngineeringLiaocheng University

drawing the ternary phase diagram of the system of 1-butyl-3-methylimidazolium tetrafluoroborate（[Bmim]BF4）,sodium dodecylbenzenesulfonate（SDBS） and water using ocular estimate and turbidity method at 298.15 K.A series of samples with different compositions around the phase diagram were *** enthalpy change of these systems after injecting triply distilled water into these samples has been studied using isothermal titration microcalorimetry（ITC）.It is found that with increasing the water content there are weak endothermic and exothermic processes appearing,separately,which matches perfectly with the location of the formationand disappearance of the aqueous two-phase in the ternary phase *** suggests the ITC is a feasible and effective method for the thermodynamic study of aqueous two-phase *** experimental results show thatthe formation of the aqueous two-phase system is endothermic（ΔH＞ 0）,but the process of disappearing is exothermic（ΔH＜ 0）.Due to the formation of aqueous two-phaseis a thermodynamic spontaneous process under the isothermal constant pressure,we can judge that the formation of this aqueous two-phase system is an entropy driven course and the vanishing is a synergy driven process by entropy and enthalpy.

关键词： Ternary phase diagram Aqueous two-phase system sodium dodecylbenzenesulfonate isothermal titration microcalorimetry

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