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Contractility detection of isolated mouse papillary muscle using myotronic Myostation-Intact device

Animal Models and Experimental Medicine 2022年第5期5卷 445-452页

作者： Hong Lian Zhuyun Qin Mengge Wu Peipei Zuo Lina Bai Minjie Lu Lulu Li Haitao Zhang State Key Laboratory of Cardiovascular Disease Fuwai HospitalNational Center for Cardiovascular DiseaseChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Beijing Key Laboratory of Preclinical Research and Evaluation for Cardiovascular Implant Materials Animal Experimental CenterFuwai HospitalNational Center for Cardiovascular DiseaseChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Adult Surgical Intensive Care Unit Fuwai HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina National Health Commission Key Laboratory of Cardiovascular Regenerative Medicine Fuwai Central-China HospitalCentral-China Subcenter of National Center for Cardiovascular DiseasesZhengzhouChina

Background:To understand the relationship between myocardial contractility and ex-ternal stimuli,detecting ex vivo myocardial contractility is necessary.Methods:We elaborated a method for contractility detection of isolated C57 mouse papillary muscle using Myostation-Intact system under different frequencies,volt-ages,and calcium concentrations.Results:The results indicated that the basal contractility of the papillary muscle was 0.27±0.03 mN at 10 V,500-ms pulse duration,and 1 Hz.From 0.1 to 1.0 Hz,con-tractility decreased with an increase in frequency(0.45±0.11-0.10±0.02 mN).The voltage-initiated muscle contractility varied from 3 to 6 V,and the contractility gradu-ally increased as the voltage increased from 6 to 10 V(0.14±0.02-0.28±0.03 mN).Moreover,the muscle contractility increased when the calcium concentration was increased from 1.5 to 3 mM(0.45±0.17-1.11±0.05 mN);however,the contractility stopped increasing even when the concentration was increased to 7.5 mM(1.02±0.23 mN).Conclusions:Our method guaranteed the survivability of papillary muscle ex vivo and provided instructions for Myostation-Intact users for isolated muscle contractility investigations.

关键词： calcium concentration frequency isolated papillary muscle muscle contractility Myostation-intact voltage

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