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Identification of protein targets for the antidepressant effects of Kai-Xin-San in Chinese medicine using isobaric tags for relative and absolute quantitation

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Neural Regeneration Research 2020年第2期15卷 302-310页

作者： Xian-Zhe Dong Dong-Xiao Wang Tian-Yi Zhang Xu Liu Ping Liu Yuan Hu Department of Clinical Pharmacology and Pharmacy Center of PharmacyChinese PLA General HospitalBeijingChina Department of Pharmacy Xuanwu Hospital of Capital Medical UniversityBeijingChina

Kai-Xin-San consists of Ginseng Radix, Polygalae Radix, Acori Tatarinowii Rhizoma, and Poria at a ratio of 3:3:2:2. Kai-Xin-San has been widely used for the treatment of emotional disorders in China. However, no studies have identified the key proteins implicated in response to Kai-Xin-San treatment. In this study, rat models of chronic mild stress were established using different stress methods over 28 days. After 14 days of stress stimulation, rats received daily intragastric administrations of 600 mg/kg Kai-Xin-San. The sucrose preference test was used to determine depression-like behavior in rats, while isobaric tags were used for relative and absolute quantitation-based proteomics to identify altered proteins following Kai-Xin-San treatment. Kai-Xin-San treatment for 2 weeks noticeably improved depression-like behaviors in rats with chronic mild stress. We identified 33 differentially expressed proteins: 7 were upregulated and 26 were downregulated. Functional analysis showed that these differentially expressed proteins participate in synaptic plasticity, neurodevelopment, and neurogenesis. Our results indicate that Kai-Xin-San has an important role in regulating the key node proteins in the synaptic signaling network, and are helpful to better understand the mechanism of the antidepressive effects of Kai-Xin-San and to provide objective theoretical support for its clinical application. The study was approved by the Ethics Committee for Animal Research from the Chinese PLA General Hospital(approval No. X5-2016-07) on March 5, 2016.

关键词： brain-derived neurotrophic factor signal pathway depression isobaric tags for relative and absolute quantitation Kai-Xin-San neurogenesis protein network proteomics analysis synaptic plasticity traditional Chinese medicine

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Comparison of isobaric and Non-isobaric Operations on a Two-Phase Dynamic Model for PEFC Cathode Gas Diffusion Layer

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Journal of Donghua University(English Edition) 2011年第6期28卷 539-546页

作者：纪光霁陈翌周苏陈凤祥 School of Automotive Studies Tongji University Chinesisch-Deutsche Hochschulkolleg Tongji University

Key words,： Two 1-D dynamical and isothermal models of cathode gas diffusion layer（GDL） with isobaric and non-isobaric operations for polymer electrolyte fuel cells（PEFCs） were developed and implemented in COMSOL Multiphysics *** artificial diffusion coefficient was introduced as well to make the numerical computation be *** the non-isobaric model,the pressure of gas mixture was obtained by summing up the governing equations of gaseous components,instead of Navier-Stoks *** of the two models were carried out with the steady-states and dynamical simulations under given *** corresponding analysis based on the simulated results was also given *** paper is contributed to finding the differences between the isobaric and non-isobaric operation in the two-phase model of cathode GDL.

关键词： two-phase model gas diffusion layer(GDL) isobaric and non-isobaric operations polymer electrolyte fuel cell(PEFC)

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Different protein expression patterns in rat spinal nerves during wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling

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Neural Regeneration Research 2020年第2期15卷 315-323页

作者： Shuai Wei Xue-Zhen Liang Qian Hu Wei-Shan Wang Wen-Jing Xu Xiao-Qing Cheng Jiang Peng Quan-Yi Guo Shu-Yun Liu Wen Jiang Xiao Ding Gong-Hai Han Ping Liu Chen-Hui Shi Yu Wang The First Affiliated Hospital of Medical College Shihezi UniversityShiheziXinjiang Uygur Autonomous RegionChina Institute of Orthopedics Chinese PLA General HospitalBeijingChina Co-Innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina The First Clinical Medical School Shandong University of Traditional Chinese MedicineJinanShandong ProvinceChina Kunming Medical University KunmingYunnan ProvinceChina Shanxi Medical University TaiyuanShanxi ProvinceChina

Sensory and motor nerve fibers of peripheral nerves have different anatomies and regeneration functions after injury. To gain a clear understanding of the biological processes behind these differences, we used a labeling technique termed isobaric tags for relative and absolute quantitation to investigate the protein profiles of spinal nerve tissues from Sprague-Dawley rats. In response to Wallerian degeneration, a total of 626 proteins were screened in sensory nerves, of which 368 were upregulated and 258 were downregulated. In addition, 637 proteins were screened in motor nerves, of which 372 were upregulated and 265 were downregulated. All identified proteins were analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of bioinformatics, and the presence of several key proteins closely related to Wallerian degeneration were tested and verified using quantitative real-time polymerase chain reaction analyses. The differentially expressed proteins only identified in the sensory nerves were mainly relevant to various biological processes that included cell-cell adhesion, carbohydrate metabolic processes and cell adhesion, whereas differentially expressed proteins only identified in the motor nerves were mainly relevant to biological processes associated with the glycolytic process, cell redox homeostasis, and protein folding. In the aspect of the cellular component, the differentially expressed proteins in the sensory and motor nerves were commonly related to extracellular exosomes, the myelin sheath, and focal adhesion. According to the Kyoto Encyclopedia of Genes and Genomes, the differentially expressed proteins identified are primarily related to various types of metabolic pathways. In conclusion, the present study screened differentially expressed proteins to reveal more about the differences and similarities between sensory and motor nerves during Wallerian degeneration. The present findings could provide a reference point for a fut

关键词： gene ontology Kyoto Encyclopedia of Genes and Genomes isobaric tags for relative and absolute quantitation motor nerve proteomics sensory nerve spinal nerve Wallerian degeneration

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Probing Temperature from Intermediate Mass Fragment by isobaric Yield Ratio in Heavy-Ion Collisions

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Communications in Theoretical Physics 2015年第12期64卷 727-730页

作者：于勉魏慧玲马春旺 Department of Life Sciences and Technology Xinxiang Medical University College of Physics and Electronic Engineering Henan Normal University

A probe to extract the temperature(T_(iyr)) from intermediate-mass fragment has been proposed using the isobaric yield ratio(IYR) difference based on the modified Fisher *** analyzing the isobaric cross section in the measured 140 A MeV^(40,48)Ca+~9Be reactions,the Tiyr can well fit the IYR difference for the large mass *** of the fragments are produced in different colliding regions,it is also suggested to select the fragments according to different mechanisms when using TIYR.

关键词： temperature isobaric yield ratio modified Fisher m

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Isotopic Ratio, Isotonic Ratio, isobaric Ratio and Shannon Information Uncertainty

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Communications in Theoretical Physics 2014年第11期61卷 717-720页

作者：马春旺魏慧玲 Institute of Particle and Nuclear Physics Henan Normal University

The isoscaling and the isobaric yield ratio difference(IBD) probes, both of which are constructed by yield ratio of fragment, provide cancelation of parameters. The information entropy theory is introduced to explain the physical meaning of the isoscaling and IBD probes. The similarity between the isoscaling and IBD results is found, i.e.,the information uncertainty determined by the IBD method equals to β- α determined by the isoscaling(α(β) is the parameter fitted from the isotopic(isotonic) yield ratio).

关键词： Shannon information isotopic ratio isotonic ratio isobaric ratio

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The isobaric Vapor-Liquid Equilibrium of Butanone-DMF and Toluene-DMF at Reduced Pressures

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Chinese Journal of Chemical Engineering 2006年第6X期14卷 825-828页

作者：刘畅王丽华何潮洪叶向群

The isobaric vapor-liquid equilibrium data of butanone(1)-N, N-dimethylformamide (DMF)(2) at 100.92kPa, 93.32kPa, and 79.99kPa and of toluene(1)-DMF(2) at 100.92kPa were measured using a modified Rose-Williams still. The above data met the thermodynamic consistency test and were correlated with the Wilson,NRTL, and UNIQUAC equations. These data can be used in the analysis and design of the process that involves separating DMF from butanone and toluene in the leather synthesis industry.

关键词： isobaric vapor-liquid equilibrium, N, N-dimethylformamide, DMF, butanone, toluene, Wilson equation, NRTL equation, UNIQUAC equation

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Effect of inlet rotating swirl on endwall film cooling for two representative hole arrangements

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Chinese Journal of Aeronautics 2018年第5期31卷 1095-1108页

作者： Yang ZHOU Yang ZHANG Xinrong SU Xin YUAN Key Laboratory for Thermal Science and Power Engineering of Ministry of Education Tsinghua University

The swirl generator is widely used in lean-burn combustor to guarantee flame stability and reduce NOxemissions. Thus, the non-uniformities induced by the swirler would affect and damage film-cooling effectiveness on the turbine components, even blow-off coolant coverage, to some certain extent. The arrangement of film-cooling holes was normally designed to be perpendicular to the axial direction and in standard straight row. In this work we experimentally studied the effects of inlet swirl and mass flow ratio on traditional film cooling holes arrangement and a new arrangement pattern whose holes are located along the isobars. Results indicated that the swirl perturbation would damage film coverage. The film-cooling effect of endwall on which the holes are along the isobars, is invariably more promising than that of endwall on which hole arrangement is perpendicular to axial with the same mass flow of coolant, whether the inlet conditions is uniform or not.

关键词： Films-cooling Holes locating isobaric Swirling flow Turbine design

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Residue Coulomb Interaction Among Isobars and Its Influence in Symmetry Energy of Neutron-Rich Fragment

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Communications in Theoretical Physics 2015年第9期64卷 334-340页

作者：马春旺王闪闪张艳丽赵艺龙魏慧玲 Institute of Particle and Nuclear Physics Henan Normal University Shanghai Institute of Applied Physics Chinese Academy of Sciences College of Physics and Electronic Engineering Henan Normal University

The residue Coulomb interaction(RCI), which affects the result of symmetry-energy coefficient of neutronrich nucleus in isobaric yield ratio(IYR) method, is difficult to be determined. Four RCI approximations are investigated:(i) The M1–RCI adopting the ac/T(the ratio of Coulomb energy coefficient to temperature) determined from the IYR of mirror-nucleus fragments;(ii) The M2–RCI by fitting the difference between IYRs;(iii) The M3–RCI adopting the standard Coulomb energy at a temperature T = 2 Me V; and(iv) Neglecting the RCI among isobars. The M1–, M2– and M3–RCI are no larger than 0.4. In particular, the M2–RCI is very close to zero. The effects of RCI in asym/T of fragment are also studied. The M1– and M4–asym/T are found to be the lower and upper limitations of asym/T, respectively. The M2–asym/T overlaps the M4–asym/T, which indicates that the M2–RCI is negligible in the IYR method, and the RCI among the three isobars can be neglected. The relative consistent low values of M3–asym/T(7.5 ± 2.5) are found in very neutron-rich isobars.

关键词： isobaric yield ratio residue Coulomb interaction s

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iTRAQ-based proteomics analysis of aqueous humor in patients with dry age-related macular degeneration

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International Journal of Ophthalmology(English edition) 2019年第11期12卷 1758-1766页

作者： Si-Chang Qu Ding Xu Ting-Ting Li Jing-Fa Zhang Fang Liu Department of Ophthalmology of Shanghai Tenth People’s Hospital Tongji Eye InstituteTongji University School of MedicineShanghai 200072China Department of Ophthalmology Shanghai General Hospital(Shanghai First People’s Hospital)Shanghai Jiao Tong UniversityShanghai 200080China

AIM: To preliminarily test proteomics in aqueous humor in patients with dry age-related macular degeneration(AMD) by using the proteomic ***: Aqueous humor samples were collected from patients with or without dry AMD, who underwent cataract surgery. The aqueous samples were analyzed with isobaric tags for relative and absolute quantification(i TRAQ) combined with liquid chromatography tandem mass spectrometry(LC-MS/MS) technology. The differential expressed proteins were analyzed with gene ontology(GO) enrichment, Kyoto Encyclopedia of Genes and Genomes(KEGG) and protein-protein interaction(PPI) network analysis. The data were partly validated by ELISA and Western blot. False discovery rate(FDR) was used for statistical analysis. RESULTS: A total of 244 proteins were detected, in which 38 proteins were up-regulated and 51 were down-regulated significantly in patients with dry AMD compared with that in control groups(FDR value <1.0%). Several proteins, e.g., protein S100-A8(S10 A8), dystroglycan(DAG1), Ig alpha-1 chain C region(IGHA1), carbonic anhydrase 3(CAH3) and alpha-1-acid glycoprotein(A1 AG1) were increased more than 5 times of that in control group. The bioinformatics analysis showed that dry AMD is closely associated with inflammation or immune reaction, oxidative stress, blood coagulation and remodeling of extracellular ***: i TRAQ-based proteomic analysis of aqueous humor demonstrate the differential expressions of proteins between dry AMD and control groups, providing the clues to understand the mechanisms and possible treatments of dry AMD.

关键词： age-related macular degeneration protein biomarker isobaric tags for relative and absolute quantification differential expression of proteins aqueous humor

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Analyzing proteins in colonic tissues from mice with ulcerative colitis using the iTRAQ technology

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Oncology and Translational Medicine 2019年第1期5卷 6-11页

作者： Na Chen Zhongyin Zhou Department of Gastroenterology Renmin Hospital of Wuhan University Hubei Key Laboratory of Digestive Disease

Objective The aim of the study was to investigate the expression of proteins in colonic tissues of mice with ulcerative colitis(UC) by using isobaric tags for relative and absolute quantitation(iTRAQ), probe into the pathogenesis of UC, and find potential biomarkers of UC. Methods Forty C57 mice were randomly divided into the control and model groups(20 mice in each group). The mice in the model group were administered dextran sulphate sodium(DSS) for 7 consecutive days ad libitum to induce acute colitis, and the colon tissue was extracted on the 8 th day after the successful establishment of the UC model. Proteins were identified by the i TRAQ and tandem mass spectrometry techniques,and the identified proteins were analyzed by bioinformatics. Results A total of 4019 proteins were identified among the two groups. Among them, 317 significant differentially expressed proteins(DEPs) were detected according to the screening criteria for selecting DEPs, i.e. fold change ratios ≥ 1.5 or ≤ 0.67 and P-values < 0.05, of which 156 were upregulated and 161 were downregulated. In the Gene Ontology(GO) analysis, the DEPs were classified into 48 functional categories, which contained biological process, cellular component, and molecular function. Based on the 317 DEPs, the KEGG pathway analysis identified 160 vital *** DEPs in colonic tissues of mice with UC were screened using the iTRAQ technique, which laid a foundation for further studies regarding the pathogenesis of UC.

关键词： ulcerative colitis (UC) isobaric tags for relative and absolute quantitation (iTRAQ) colonic tissue differentially expressed proteins (DEPs)

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