检索结果-南通市图书馆

Inhibition of IKK-NFκB pathway sensitizes lung cancer cell lines to radiation

Cancer Biology & Medicine 2017年第3期14卷 293-301页

作者： Avgi Tsolou Maria Liousia Dimitra Kalamida Stamatia Pouliliou Alexandra Giatromanolaki MichaelKoukourakis Department of Radiotherapy/Oncology Democritus University of Thrace and University General Hospital of AlexandroupolisAlexandroupolis 68100Greece Department of Pathology Democritus University of Thrace and University General Hospital of AlexandroupolisAlexandroupolis 68100Greece

Objective:Cancer cell radioresistance is a stumbling block in radiation *** activity in the nuclear factor kappa B(NFκB)pathway correlates with anti-apoptotic mechanisms and increased *** IKK complex plays a major role in NFκB activation upon numerous *** this study,we examined the interaction between ionizing radiation(IR)and different members of the IKK-NFκB pathway,as well as upstream activators,RAF1,ERK,and ***:The effect of 4 Gy of IR on the expression of the RAF1-ERK-IKK-NFκB pathway was examined in A549 and H1299 lung cancer cell lines using Western blot analysis and confocal *** examined changes in radiation sensitivity using gene silencing or pharmacological inhibitors of ERK and IKKβ.Results:IKKα,IKKγ,and IκBαincreased upon exposure to IR,thereby affecting nuclear levels of NFκB(phospho-p65).ERK inhibition or si RNA-mediated down-regulation of RAF1 suppressed the post-irradiation survival of the examined lung cancer cell lines.A similar effect was detected on survival upon silencing IKKα/IKKγor inhibiting IKKβ.Conclusions:Exposure of lung cancer cells to IR results in NFκB activation via *** genetic or pharmacological blockage of the RAF1-ERK-IKK-NFκB pathway sensitizes cells to therapeutic doses of ***,the IKK pathway is a promising target for therapeutic intervention in combination with radiotherapy.

关键词： IKK NFκB ionising radiation lung cancer radiosensitization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Alpha-2-macroglobulin as a radioprotective agent: a review

引用

Chinese Journal of Cancer Research 2014年第5期26卷 611-621页

作者： Xueying Chen Xiangbo Kong Zhaoqiang Zhang Wei Chen Jieyu Chen Huanyang Li Wanting Cao Yaping Ge Silian Fang Department of Oral and Maxillofacial Surgery The Sixth Affiliated Hospital of Sun Yat-Sen University Department of Periodontology Sun Yat-Sen Memorial HospitalSun Yat-Sen University Department of Stomatology Zhuhai HospitalGuangdong Provincial Hospital of Traditional Chinese Medical

radiation is an important modality in cancer treatment, and eighty percent of cancer patients need radiotherapy at some point during their clinical management. However, radiation-induced damage to normal tissues restricts the therapeutic doses of radiation that can be delivered to tumours and thereby limits the effectiveness of the treatment. The use of radioprotectors represents an obvious strategy to obtain better tumour control using a higher dose in radiotherapy. However, most of the synthetic radioprotective compounds studied have shown inadequate clinical efficacy owing to their inherent toxicity and high cost. Hence, the development of radioprotective agents with lower toxicity and an extended window of protection has attracted a great deal of attention, and the identification of alternative agents that are less toxic and highly effective is an absolute necessity. Recent studies have shown that alpha-2-macroglobulin（α2M） possesses radioprotective effects. α2M is a tetrameric, disulfide-rich plasma glycoprotein that functions as a nonselective inhibitor of different types of non-specific proteases and as a carrier of cytokines, growth factors, and hormones. α2M induces protein factors whose interplay underlies radioprotection, which supports the idea that α2M is the central effector of natural radioprotection in the rat. Pretreatment with α2M has also induced a significant reduction of irradiation-induced DNA damage and the complete restoration of liver and body weight. Mihailovi？ et al. concluded that the radioprotection provided by α2M was in part mediated through cytoprotection of new blood cells produced in the bone marrow; these authors also indicated that an important aspect of the radioprotective effect of amifostine was the result of the induction of the endogenous cytoprotective capability of α2M. The radioprotective effects of α2M are possibly due to antioxidant, antifibrosis, and anti-inflammatory functions, as well as the maintenance of homeostasis

关键词： Alpha-2-macroglobulin(α 2 M) ionising radiation radioprotection radiation-induced fibrosis mechanism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Radiotherapy and the cellular DNA damage response: current and future perspectives on head and neck cancer treatment

引用

Cancer Drug Resistance 2020年第4期3卷 775-790页

作者： Maria Rita Fabbrizi Jason L.Parsons Cancer Research Centre Department of Molecular and Clinical Cancer MedicineUniversity of LiverpoolLiverpool L39TAUnited Kingdom Clatterbridge Cancer Centre NHS Foundation Trust Clatterbridge RoadBebington CH634JYUnited Kingdom

Incidences of head and neck squamous cell carcinoma(HNSCC)have been on the rise in the last few decades,with a significant risk factor being human papillomavirus(HPV)type-16/18 infection,particularly in the development of oropharyngeal ***(RT)is an important treatment modality for HNSCC,where it promotes extensive cellular DNA damage leading to the therapeutic *** has been well-established that HPV-positive HNSCC display better response rates and improved survival following RT compared to HPV-negative *** differential radiosensitivity has been largely associated with altered cellular DNA damage response mechanisms in HPV-positive HNSCC,and particularly with the signaling and repair of DNA double strand ***,other factors,particularly hypoxia present within the solid cancer,have a major impact on relative ***,recent approaches aimed at enhancing the radiosensitivity of HNSCC have largely centered on targeting key proteins involved in DNA repair,DNA damage checkpoint activation,and hypoxia *** studies have utilised in vitro and in vivo models of HPV-positive and HPV-negative HNSCC and examined the impact of specific inhibitors against the targets in combination with radiation in suppressing HNSCC cell growth and ***,accumulating evidence has shown that targeting enzymes including poly(ADP-ribose)polymerase,ataxia telangiectasia and Rad-3 related,DNA-dependent protein kinase catalytic subunit,and checkpoint kinase 1 can radiosensitise HNSCC cells which should be taken forward in further preclinical studies,with the goal of optimizing the future effective RT treatment of HNSCC.

关键词： DNA damage DNA repair head and neck cancer ionising radiation proton beam therapy radiobiology radiotherapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of Low Gamma Irradiation on the Germination and Morphological Characteristics of Broad Beans (Vicia faba L.), Mung Beans (Vigna radiata L.), and Peas (Pisum sativum L.) Seedlings

引用

Natural Resources 2022年第5期13卷 105-125页

作者： Abidemi Atteh Adesoji Adeyeye Department of Biological and Environmental Sciences University of Stirling Stirling Scotland UK

Understanding the effects of ionising radiation (IR) on plants has been a major focus of research. Acute high-dose effects are well-documented and understood (mainly through laboratory testing). Lower doses, on the other hand, are less understood, as low dosage research is controversial, and there are only a few studies that use low and ecologically relevant IR levels, particularly those conducted under controlled conditions. The effect of low gamma radiation was investigated in this study using Vicia faba L., Vigna radiata L., and Pisum sativum L. Healthy and viable seeds of these plants were irradiated with varying doses of gamma radiation (Cs−137 source) and sown under controlled environmental conditions. The doses/dose rates used were within the scope of the International Commission on Radiological Protection’s Derived Consideration Reference Level (DCRL) for these groups of plants (1 - 10 mGy∙d−1), so this study tested this DCRL. Observations were made on certain germination parameters and growth traits like germination percentage and rate, shoot and root length, seed weight, number of leaves, wet and dry biomass, plant height, leaf chlorophyll content, and leaf area. In the germination phase, the doses employed in this experiment did not affect the seeds’ weight, germination percentage, and rate, but there were some interesting effects on the root and shoot length;as all irradiated groups performed better than the control group (particularly the 16.2 mGy and 48.5 mGy dose in V. radiata and P. sativum, while the 1070 mGy dose had the highest value in V. faba). However, the plants were able to compensate for the effects observed in the germination phase and by the end of the experiment, there were no statistically significant effects (at 0.05 p level) in all the morphometric parameters studied;the visible organs appeared normal, and growth rate was normal. This study, therefore, concludes that the DCRL used to p

关键词： ionising radiation Morphometric Parameters Germination Parameters ICRP DCRLs

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：