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Plasma Science and Technology 2007年第2期9卷 235-240页

作者：张祥胜吴李君余立祥魏胜林刘竟男余增亮 Key Laboratory of ion beam Bio-Engineering Institute of Plasma PhysicsChinese Academy of SciencesHefei 230031China College of Biological ScienceYangtze UniversityJingzhou 434025China Key Laboratory of ion beam Bio-Engineering Institute of Plasma PhysicsChinese Academy of SciencesHefei 230031China

Ar^＋ ion beam with low energy of 30 keV was implanted into liquorice （Glycyrrhiza uralensis Fisch） seeds at the doses of 0, 600, 900 and 1200 × （2.6 × 10^13） ions/cm^2, respectively. The seeds were sowed in pots and after one month the plants were subjected to different drought conditions for two months. Then the plants＇ morphological and physiological characteristics, antioxidation enzymes and levels of endogenous hormones were investigated. The results showed that ion implantation at a proper dose can greatly enhance the liquorice seedlings＇ resistance against drought stress.

关键词： ion beam implantation drought tolerance Glycyrrhiza uralensis Fisch antioxidation enzyme endogenous hormone

Enhancement of Gongronella *** Chitosanase Production by ion beam implantation

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Plasma Science and Technology 2007年第1期9卷 115-118页

作者：袁航周伟王军张束清姚建铭 Key Laboratory of ion beam Bioengineering Chinese Academy of SciencesHefei 230031China

Gongronella sp. JG was a fungal strain which expressed extracellular chitosanase of about 800 U/L during its growth in production medium. To improve its enzyme production, low energy N＋ implantation was employed to mutate spores of JG. The implantation condition was optimized and the parameters of 15 keV and 60 × breeding experiments. A mutant designated as SG 2.6 × 10^13 ions/cm^2 were selected for further was obtained. It showed increased chitosanase production （1800 U/L） and shortened cultivation period （from 72 h to 60 h）. Five-generation cultivation of SG indicated that its chitosanase production was stable at about 1800 U/L.

关键词： breeding chitosanase Gongronella sp. ion beam implantation

Study on the Variation of the Distant Crossing Rice by ion beam implantation

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Plasma Science and Technology 2005年第2期7卷 2785-2788页

作者：吴跃进张瑛吴敬德童继平李红郑乐娅宋美余增亮 Key Laboratory of ion beam Bioengineering Institute of Plasma Physics Chinese Academy of SciencesHefei 230031China Key Laboratory of ion beam Bioengineering Institute of Plasma Physics Chinese Academy of SciencesHefei 230031China Anhui Academy of Agricultural SciencesKey Laboratory of Rice Genetics & Breeding of Anhui ProvinceInstitute of Rice ResearchHefei 230031China Anhui Academy of Agricultural Sciences Key Laboratory of Rice Genetics & Breeding of Anhui Province Institute of Rice ResearchHefei 230031China

In this paper, the following contents including the original receptor EI213 and other two control materials, RAPD polymorphism, photosynthetic efficiency, and the number of vascular bundles of the first internodes below the peduncle have been studied for the eight F7 transgenic lines obtained from ion beam implantation. The results showed that there was a significant variation in genomes of maize-rice line, compared with the receptor EI213, after the total exogenous maize DNA was introduced into EI213. The number of the vascular bundles of maize-rice progeny’s lines was obviously much larger than those of the original receptor EI213 and other two controls GER-3 and MH63, and along with the photosynthetic efficiency of maize-rice progeny’s lines being gone up. Moreover, the parenchyma cells around the vascular bundles of the transgenic lines became much larger in number and in size than those around the controls. All these indicated that the maize-rice progeny’s lines are really different from and superio

关键词： rice ion beam implantation progeny variation

Screening of Biocontrol Strain Bacillus subtilis by N^+ ion beam implantation

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Agricultural Science & Technology 2012年第8期13卷 1658-1663页

作者：刘桂君孟佑婷杨素玲包放尚宏忠北京市辐射中心微生物辐射育种实验室北京100015

[Objective] This study was to investigate the effect of N＋ ion beam implantation on the survival rate and mutation rate of biocontrol strain Bacillus subtilis. [Method] The factors influencing B. subtilis ion beam implantation, including culture time, dilution concentration, solvent, drying time of mycoderm were optimized. B. subtilis cells were implanted by using ion beam at dose of 2.0×10^14～4.0×10^14 ions/cm2 and the energy of 30 kev. Then the methods of culturing colonies confronting each other on plate and Oxford cup diffusion were used to screening strains. [Result] The optimal parameters were found as follows： culture in liquid for 20-24 h, dilution with sterile water to 106 cells/ml and drying time of 60 min for sample preparation; the optimal N＋ ion beam implantation dose of 2.0×10^14～4.0×10^14 ions/cm2 at the energy of 30 kev, the survival rate of 8.43%-26.71% and the mutation rate of 3.50%-5.43%. [Conclusion] This study provided reference for ion beam implantation mutation of B. subtilis.

关键词： ion beam implantation Survival rate Mutation rate Bacillus subtilis

Modification of the Properties of Cu_x S Thin Films by Low Energy ion beam implantation

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Semiconductor Photonics and Technology 2000年第3期6卷 156-160页

作者： CAO Meng, ZHENG Jian-bang, LIU Xiao-zeng, LI Ning, WU Hong-cai (School of Electron & Information Engineering, Xi’an Jiaotong University , Xi’an 710049, CHN) 西安交通大学电信学院光电技术与太阳能研究所陕西西安 710049

Cu x S thin films are implanted by low energy N + ion beam. The influences of the energy and dose of N + ion implantation on Cu x S films are investigated. The results show that the ratio of copper to sulfur is increased to some extent, the constituents of the film are turned to rich copper phase from rich sulfurous phase after ion beam irradiation. X-ray diffraction spectrum and optical transmission spectra of sample have confirmed the results.

关键词： ion beam implantation Cu x S thin film Rich copper phase fil\

Purification and Properties of a New L-Sorbose Dehydrogenase Accelerative Protein from Bacillus megaterium Bred by ion-beam implantation

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Plasma Science and Technology 2008年第3期10卷 398-402页

作者：赵世光姚黎明苏彩欣王陶王军汤明礼余增亮 Key Laboratory of ion beam Bioengineering Institute of Plasma Physics Chinese Academy of Sciences Hefei 230031 China

Bacillus megaterium BM302 bred by ion-beam implantation produces L-sorbose dehydrogenase accelerative protein （SAP） to accelerate the activity of L-sorbose dehydrogenase （SDH） of Gluconobacter oxydans in the 2-keto-L-gulonic acid （2KLG） fermentation from L-sorbose by the mixed culture of B. megaterium BM302 and G. oxydans. The SAP purified by three chromatographic steps gave 35-fold purification with a yield of 13% and a specific activity of 5.21 units/mg protein. The molecular weight of the purified SAP was about 58 kDa. The SDH accelerative activity of SAP at pH 7 and 50℃ was the highest. Additionally, it retained 60% activity at a pH range of 6.5 ～ 10 and was stable at 20℃ ～ 60℃. After 0.32-unit SAP was added to the single cultured G. oxydans strains, the SDH activity was apparently accelerated and the 2KLG yield of GO29, GO112, GO and GI13 was enhanced 2.1, 3.3, 3.5 and 2.9 folds respectively over that of the strains without the addition of SAP.

关键词： bacillus megaterium BM302 ion beam implantation counterpart protein two-step fermentation of Vc

Impact of ion implantation on Licorice(Glycyrrhiza uralensis Fisch) Growth and Antioxidant Activity Under Drought Stress

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Plasma Science and Technology 2007年第3期9卷 301-306页

作者：刘竞男童丽萍沈童伟李杰吴李君余增亮 Key Laboratory of ion beam Bioengineering Institute of Plasma PhysicsChinese Academy of SciencesHefei 230031China School of Life ScienceAnhui UniversityHefei 230039China School of Life Science Anhui UniversityHefei 230039China Key Laboratory of ion beam Bioengineering Institute of Plasma PhysicsChinese Academy of SciencesHefei 230031China

Low energy ion beams are known to have stimulation effects on plant generation and to improve plants＇ intrinsic quality. In the present study, the growth and physiological index of licorice implanted with 0, 8, 10; 12 and 14× （2.6× 10^15） ions/cm^2 were investigated under well-watered and drought-stress conditions. The results showed that a proper dose of ion implan- tation was particularly efficient in stimulating the licorice growth and improving the plant biomass significantly in both the well-watered and drought-stress conditions. The physiological results of licorice measured by leaf water potential, lipid oxidation, soluble protein and antioxidant system showed a significant correlation between ion implantation and water regime except for leaf water potential. Therefore, the study indicated that ion implantation can enhance licorice＇s drought tolerance by increasing the activity of superoxide dismutase （SOD）, eatalase （CAT） and DPPH （1,1-diphenyl-2-picrylhydrazyl） radical scavenging ability to lower oxidative damage to lipids in plants. ion beam implantation, therefore, provides an alternative method to enhance licorice drought tolerance.

关键词： licorice (Glycyrrhiza uralensis Fisch) oxidative stress drought tolerance ion beam implantation

Mutation Breeding of β-carotene Producing Strain B. trispora by Low Energy ion implantation

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Plasma Science and Technology 2009年第1期11卷 110-115页

作者：张宁虞龙 College of Life Science and Pharmaceutical Engineering Nanjing University of Technology

ion beam bioengineering technology as a new mutation approach has been widely used in the biological breeding field. In this paper the application of low energy nitrogen ion implantation in the β-carotene producing strain, Blakeslea trispora（-） was investigated. The effects of different fermentation conditions on β-carotene production by a high yield strain were examined. Results showed that two β-carotene high yielding strains ***（-） BH3-701 and BH3-728 were screened out and the averaged production of β-carotene was raised by 178.7% and 164.6% respectively after five passages in the shaking flasks. Compared with the original strain, the highest yield strain BH3-701 was potent in accumulating β-carotene, especially in the later stage, and greatly increased production efficiency.

关键词： blakeslea trispora,β-carotene ion beam implantation mutation screen

Study of Genetics and Embryology of Polyembryonic Mutant of Autotetraploid Rice Induced by N^+ beam implantation

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Plasma Science and Technology 2006年第6期8卷 745-750页

作者：代西梅黄群策李国平胡秀明秦广雍余增亮 Henan Provincial Key Laboratory of ion beam Bio-Engineering Zhengzhou University Zhengzhou 450052 China Institute of Plasma Physics Chinese Academy of Sciences Hefei 230031 China

In the present study autotetraploid rice IR36-4X was treated by an ion implantation technique with nitrogen ion beams. A polyembryonic mutant （named IR36-Shuang） was identified in the M2 generation. The mutant line and its offspring were systematically investigated in regard to their major agronomic properties and the rate of polyembryonic seedling in the M3-M6 generation. The abnormal phenomena in the embryo sac development and the cytological mechanism of the initiation of additional embryo in IR36-Shuang were observed by Laser Scanning Confocal Microscopy. The results were as follows. 1） The plant height, the panicle length and 1000 grain weight of IRB6-Shuang were lower than that of its control by 35.41%, 5.08% and 15.72% respectively, Moreover, the setting percentage decreased 12.39% compared with that in normal IRB6-4X plants. 2） The polyembryonic trait of IRB6-Shuang was genetically stable and the frequency of the polyembryonic seedlings in the IR36-Shuang line was also relatively stable. 3） The rate of abnormal embryo sacs in IR36-Shuang was significantly higher than that in the control IR36-4X. 4） The additional embryo in IR36-Shuang might arise from the double Jet of embryo sacs in a single owry, antipodal cells or endosperm cells. These results suggest that IRB6-Shuang is a polyembryonic mutant and a new apomixis rice line induced by low energy ion implantation. The prospects for the application in production of the IR36-Shuang line are also discussed. The present study may provide a basis for future investigations of apomixis rice breeding via the ion implantation biotechnology.

关键词： ion beam implantation mutant, polyembryonic seedling, autotetraploid rice, embrvogenesis

Enhancement of L(+)-Lactic Acid Production of Immobilized Rhizoous Orvzae Imolanted by ion beams

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Plasma Science and Technology 2008年第1期10卷 136-140页

作者：樊永红杨英歌郑之明李文王鹏姚黎明余增亮 Key Laboratory of ion beam Bioengineering Institute of Plasma Physics Chinese Academy of Sciences Hefei 230031 China Xinjiang University Urumqi 830046 China Key Laboratory of ion beam Bioengineering Institute of Plasma Physics Chinese Academy of Sciences Hefei 230031 China

Immobilized Rhizopus oryzae culturing may be a solution to the inhibited production of L（＋）-lactic acid in submerged fermentation, which is caused by aggregated mycelia floc. In the present study, a R. oryzae mutant （RL6041） with a 90% conversion rate of glucose into L-lactic acid was obtained by N＋ implantation under the optimized conditions of a beam energy of 15 keV and a dose of 2.6 ×10^15 ions/cm^2. Using polyurethane foam as the immobilization matrix, the optimal L-lactic acid production conditions were determined as 4 mm polyurethane foam, 150 r/min, 50 g/L ～ 80 g/L of initial glucose, 38℃ and pH 6.0. 15-cycle repeated productions of L-lactic acid by immobilized RL6041 were performed under the optimized culturing conditions and over 80% of the glucose was converted into L-lactic acid in 30 hours on average. The results show that immobilized RL6041 is a promising candidate for continuous L-lactic acid production.

关键词： immobilized Rhizopus oryzae L-lactic acid ion beam implantation