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In vitro maturation of human oocytes maintaining good development potential for rescue intracytoplasmic sperm injection with fresh sperm

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World Journal of Clinical Cases 2022年第7期10卷 2166-2173页

作者： Yun-Qiao Dong Chuang-Qi Chen Yu-Qiang Huang Dun Liu Xi-Qian Zhang Feng-Hua Liu Department of Assisted Reproductive Center Guangdong Women and Children HospitalGuangzhou 511400Guangdong ProvinceChina

BACKGROUND The outcomes of the use of commercial in vitro maturation(IVM)medium to culture immature oocytes obtained from conventional ovulation induction,followed by rescue intracytoplasmic sperm injection(RICSI),are not ideal.It is thus difficult to widely adopt this approach in clinical practice.Therefore,it is necessary to explore methods for improving the clinical outcome of IVM.AIM To study the effect of sperm on the developmental potential of in vitro-matured oocytes in conventional culture.METHODS This was a retrospective study of patients whose immature oocytes were harvested from conventional oocyte stimulation cycles and underwent ICSI at our hospital between June 2018 and August 2020.RICSI was performed using sperm collected on the day of oocyte harvest(old)and sperm collected on the day of RICSI(fresh)and oocytes matured in vitro after 24 h of culture in conventional medium.The rates of in vitro oocyte maturation,normal fertilization,normal cleavage,day-3 top-quality embryos,and useful blastocyst formation were compared between the two groups.RESULTS In total,102 germinal vesicle(GV)-stage immature oocytes were cultured in the old sperm group.In the fresh sperm group,122 GV-stage immature oocytes were collected and cultured in vitro for 24 h.There were no significant differences in the general conditions of males and females between the two groups(P>0.05).The oocyte maturation,normal fertilization,and normal cleavage rates of the old and fresh groups were 51.0%vs 55.7%,61.5%vs 64.7%,and 93.8%vs 93.2%,respectively.None of the rates differed significantly(P>0.05)between the two groups.However,the day-3 top-quality embryo and useful blastocyst rates of the old and fresh sperm groups were 16.6%vs 63.4%;6.67%vs 34.6%,respectively.The day-3 top-quality embryos and useful blastocyst rates of the old sperm group were significantly lower than those of the fresh group(P<0.05).CONCLUSION In vitro maturation with conventional culture medium combined with the use of fresh sperm collected on the day of RICSI is an

关键词： In vitro oocyte maturation Sperm injections intracytoplasmic Semen analysis In vitro fertilization Human oocyte

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Success rates of in vitro fertilization versus intracytoplasmic sperm injection in men with serum anti-sperm antibodies:a consecutive cohort study

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Asian Journal of Andrology 2019年第5期21卷 473-477页

作者： Shao-Ming Lu Xiao Li Shi-Li Wang Xiao-Li Yang Yan-Zhen Xu Ling-Ling Huang Jiao-Long Liu Fei-Fei Cai Zi-Jiang Chen Center for Reproductive Medicine Shandong Provincial Hospital Affiliated to Shandong UniversityNational Research Center for Assisted Reproductive Technology and Reproductive GeneticsThe Key laboratory for Reproductive Endocrinology of Ministry of EducationJinan 250000China Maternal and Child Health Care Hospital Rizhao 276800China The Medical Scientific Research Center of Guangxi Medical University Nanning 53000China Department of Obstetrics The First Affiliated Hospital of Guangxi Medical UniversityNanning 53000China

Antisperm antibodies(ASAs)are assumed to be a possible causative factor for male infertility,with ASAs detected in 5%-15%of infertile men but in only l%-2%of fertile ones.It remains unclear whether ASAs have an adverse effect on the outcome of in v/tro fertilization(IVF)or intracytoplasmic sperm injection(ICSI).This study investigated differences in the rates of fertilization,pregnancy,and live births associated with serum ASA-positive and ASA-negative men following IVF or ICSI.Five hundred and fifty-four consecutive infertile couples undergoing IVF(n=399)or ICSI(n=155)were included.The two-sample two-sided t-test and Chi-square or Fisher's exact test was used for statistical analysis.Lower rates of fertilization(41.7%vs 54.8%,P=0.03),good embryos(18.9%vs 35.2%,P=0.00),pregnancy(38.5%vs 59.4%,P=0.00),and live births(25.8%vs 42.5%,P=0.00)were observed in men of the IVF group with a positive serum ASA than in those with a negative ASA.ASA positivity/negativity correlated with pregnancy rates(P=0.021,odds ratio[OR]:0.630,95%confidence interval[Cl]:0.425-0.932)and live birth rates(P=0.010,OR:1.409,95%Cl:1.084-1.831)after controlling for the female serum follicle-stimulating hormone level and the couple's ages at IVF.Women coupled with ASA-positive men had lower live birth rates with IVF than with ICSI(25.8%and 47.4%,respectively;P=0.07).Women coupled with ASA-positive men had lower rates of pregnancy and live births following IVF than those coupled with ASA-negative men but had a similar outcome with ICSI.

关键词： intracytoplasmic sperm injection in vitro fertilization pregnancy outcome serum antisperm antibodies

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A successful pregnancy by intracytoplasmic sperm injection using ejaculate sperm from an infertile man with 46, XX/46, XY true hermaphrodite

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Asian Journal of Andrology 2017年第6期19卷 721-722页

作者： Yan-Wei Sha Yan-Kun Sha Lu Ding Shao-Bin Lin Zhi-Yong Ji Xu Wang Yue-Qiang Song Ping Li Center for Reproductive Medicine Xiamen Maternity and Child Care HospitalXiamen 361003 China Department of Nephrology First Affiliated Hospital ofLiaoning Medical University Jinzhou 121000 China.

Dear Editor, True hermaphroditism is a condition in which the gonads, genital morphology, and sexuality simultaneously show both male and female characteristics （ovaries and testes） or in which both types of gonadal tissue exist in a single gonad （known as an ovariotestis）. True hermaphroditism associated with a chimeric or 46, XX/46, XY karyotype is extremely rare, and the genitalia of those affected can be characterized as female, male, or mixed.1-3 The cause of true hermaphroditism has not been determined because the condition is relatively rare and has a diverse phenotype.4

关键词： intracytoplasmic ejaculate hermaphrodite

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intracytoplasmic sperm injection in the treatment of male infertility due to obstructive or non-obstructive azoospermia

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生殖医学杂志 2003年第Z1期12卷 51-55页

作者：刘伟信黄萍王丽罗孟军岳利民郑煜 Department of Physiology Medical CollegeSichuan UniversityChengdu 610041China Chengdu Institute of Family PlanningChengdu 610031China Chengdu Institute of Family Planning Chengdu 610031China Department of Physiology Medical CollegeSichuan UniversityChengdu 610041China

Objective: To evaluate the effects of intracytoplasmic sperm injection (ICSI) ontreatment of infertility due to obstructive and non-obstructive azoospermia..Methods: A retrospective analysis of fertilization, cleavage, embryo implantationand pregnancy rates was done in 158 ICSI cycles including 112 obstructive azoospermiaand 46 non-obstructive azoospermia. Ovarian hyperstimulation and ICSI procedureswere performed by conventional protocol. The sperm was collected by percutaneous epi-didymal sperm aspiration (PESA) or testicular sperm extraction (TESE).Results:The fertilization rate (73.1% vs. 67.0%), cleavage rate (88.6% vs. 86.3%), embryo implantation rate (20.7% vs. 11.4%), clinical pregnancy rate per trans-fer cycle (35.7% vs. 19.6%) were obtained for obstructive and non-obstructiveazoospermia, respectively.Conclusion: The results revealed that in the cases of obstructive azoospermia, ferti-lization rate, embryo implantation rate and clinical pregnancy rate were significantlyhigher than those of non-obstructive azoospermia. But there was no significant differ-ence of the cleavage rate between two groups.

关键词： intracytoplasmic sperm injection Obstructive azoospermia Non-ob-structive azoospermia Male infertility

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A comparison of the relative efficiency of ICSI and extended culture with epididymal sperm versus testicular sperm in patients with obstructive azoospermia

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Asian Journal of Andrology 2020年第2期22卷 222-226页

作者： Scott J Morin Brent M Hanson Caroline R Juneau Shelby A Neal Jessica N Landis Richard T Scott Jr James M Hotaling Reproductive Medicine Associates of New Jersey 140 Allen RoadBasking RidgeNJ 07920USA Division of Reproductive Endocrinology and Infertility Department of Obstetrics and GynecologySidney Kimmel Medical College at Thomas Jefferson University833 Chestnut StreetPhiladelphiaPA 19107USA Foundation for Embryonic Competence 140 Allen RoadBasking RidgeNJ 07920USA Division of Urology Department of SurgeryUniversity of Utah School of Medicine30 N 1900 ESalt Lake CityUT 84132USA Department of Obstetrics and Gynecology University of Utah School of Medicine30 N 1900 ESalt Lake CityUT 84132USA.An abbreviated version of this manuscript was published and presented as an abstract at the American Society for Reproductive Medicine(ASRM)2017 Scientific Congress.

This is a retrospective cohort study comparing blastocyst transfer outcomes following intracytoplasmic sperm injection utilizing epididymal versus testicular sperm for men with obstructive azoospermia.All cases at a single center between 2012 and 2016 were included.Operative approach was selected at the surgeon’s discretion and included microepididymal sperm aspiration or testicular sperm extraction.Blastocyst culture was exclusively utilized prior to transfer.The primary outcome was live birth rate.Secondary outcomes included fertilization rate,blastulation rate,euploidy rate,and implantation rate.A mixed effects model was performed.Seventy-six microepididymal sperm aspiration cases and 93 testicular sperm extraction cases were analyzed.The live birth rate was equivalent(48.6%vs 50.5%,P=0.77).However,on mixed effects model,epididymal sperm resulted in a greater likelihood of fertilization(adjusted OR:1.37,95%CI:1.05–1.81,P=0.02)and produced a higher blastulation rate(adjusted OR:1.41,95%CI:1.1–1.85,P=0.01).As a result,the epididymal sperm group had more supernumerary blastocysts available(4.3 vs 3,Pintracytoplasmic sperm injection following microepididymal sperm aspiration resulted in a greater number of usable blastocysts per patient.Thus,the true benefit of epididymal sperm may only be demonstrated via a comparison of cumulative pregnancy rates after multiple transfers from one cohort.

关键词： azoospermia intracytoplasmic sperm injection male infertility testicular sperm extraction

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Role of Dehydroepiandrosterone Supplementation in Improving intracytoplasmic Sperm Injection Outcome for Women with Expected Poor Ovarian Response

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Open Journal of Obstetrics and Gynecology 2019年第3期9卷 353-362页

作者： Abd El-Naser Abd El-Gaber Ali Mustafa M. Khodry ART Unit of Obstetrics & Gynecology Department Qena Faculty of Medicine South Valley University Qena Egypt

Background: As regard to adjuvant supplementations, nowadays dehydroepiandrosterone (DHEA) is widely used all over the world and is considered to be a potential agent to ameliorate the assisted reproduction technologies outcomes of infertile women with poor ovarian reserve. Objective: To find out the role of DHEA supplementation in improving intracytoplasmic sperm injection (ICSI) outcome for infertile women with expected poor ovarian response in controlled ovarian stimulation. Setting: Assisted reproduction unit of Obstetrics and Gynecology Department, Faculty of Medicine, South Valley University, Egypt. Duration: From April 2016 to May 2018. Study Design: A randomized double-blinded controlled trial. Methods: One hundred and forty infertile women with expected poor ovarian response prepared for ICSI procedure were included in this study. Patients were divided into two groups;group I (DHEA group) included 70 patients received 25 mg DHEA 12 weeks prior to ICSI cycle and group II (placebo group) included 70 patients received a placebo. Results: There was a highly statistically significant difference in basal AFC at start of ICSI cycle in group I (who received DHEA supplementation for 12 weeks prior to ICSI procedure) than in group II (13.8 ± 5.3 versus 10.7 ± 4.6 respectively) with P 0.05). Conclusions & Recommendations: DHEA supplementations improved basal AFC, increased the number & quality of oocytes and increased quality of embryos in infertile patients with expected poor ovarian response in ICSI procedure. So DHEA supplementations could be an important adjuvant for infertile women with expected poor ovarian response in

关键词： Dehydroepiandrosterone (DHEA) intracytoplasmic Sperm Injection (ICSI) Poor Ovarian Response (POR)

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Effects of MⅡ stage oocytes zona pellucida birefringence on pregnancy outcome

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Asian Pacific Journal of Tropical Medicine 2013年第7期6卷 578-582页

作者： Jia Luo Yan-Wen Xu Ming-Fang Zhang Ling Gao Cong Fang Can-Quan Zhou Reproductive Center of the First Affiliated Hospital of Sun Yat-sen University

Objective:To explore the effects of different MⅡstage oocytes zona pellucida birefringence on pregnancy outcome.Methods:A total of 46 couples with infertile which induced by single cause received in-vitro fertilization treatment were analyzed retrospectively,and randomly divided into the high zona birefringence(HZB)/HZB group.HZB/low zona birefringeuce(LZB) group and LZB/LZB group according to different oocytes zona pellucida birefringence.intracytoplasmic sperm injection outcome was analyzed and compared.Results:The proportion of HZB oocytes, implantation rate and the pregnancy rate were decreased in three groups(HZB/HZB group>HZB/ LZB group>LZB/LZB group)(P0.05).Logistic regression analysis showed that factors allecl MⅡstage oocytes zona pellucida birefringence were age.basal FSH level and the LH level on the day of HCG injection.Age and KSH levels were negatively correlated with the single oocyte zona pellucida birefringence:While the LH level on the day of hCC injection was positively correlated with the single oocylc zona pellucida birefringence.Conclusions:The primary influence factors on MⅡstage oocytes zona pellucida are age.basal FSH level and the LH level on the day of hCG injection.The birefringence value of zona pellucida can affect the pregnancy outcome.

关键词： Oocytes Zona pellucida intracytoplasmic sperm injection Implantation Pregnancy

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Effect of Vitamin D Supplementation on intracytoplasmic Sperm Injection Outcomes: A Randomized Double-Blinded Placebo-Controlled Trial

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Open Journal of Obstetrics and Gynecology 2018年第14期8卷 1549-1556页

作者： Ahmed H. Abd-Ellah Abd El-Naser Abd El-Gaber Ali Mustafa M. Khodry Mahmoud I. El-Rasheedy Ahmed M. Abbas ART Unit Department of Obstetrics & Gynecology Faculty of Medicine South Valley University Qena Egypt Department of Obstetrics & Gynecology Faculty of Medicine Al-Azhar University Assiut Egypt Department of Obstetrics & Gynecology Faculty of Medicine Assiut University Assiut Egypt

OBJECTIVE: To assess the effect of vitamin D supplementation on enhancement of embryos implantation rates in intracytoplasmic sperm injection procedures. STUDY DESIGN: A randomized double-blinded controlled trial. SETTING: Assisted Reproduction Unit of Obstetrics and Gynecology Department, Faculty of Medicine, South Valley University, Egypt. DURATION: From December 1st 2017 to the end of October 2018. SUBJECTS and METHDS: Eighty infertile couples that underwent ICSI procedure were entrapped in this study. Patients were divided into two groups;Group I (Vitamin D group) = 40 patients (received vitamin D supplementation in the form of Vidrop oral drops 600 IU/day starting after ovum pickup) and Group II (placebo group) = 40 patients (received a placebo “normal saline oral drop”). RESULTS: There was a significantly increased embryo implantation rates (53.2% in vitamin D group versus 46.7% in placebo group), there was a mild statistically significant difference in the clinical pregnancy rates (45.9% in vitamin D group versus 39.4% in placebo group) with p-value < 0.05 and there was an improvement in ongoing pregnancy rate as there was a mild statistically significant difference in first trimester miscarriage rates (12.5% in vitamin D group versus 17.5% in placebo group) with p value CONCLUSIONS: Vitamin D supplementation could improve embryos implantation rates and rate of ongoing pregnancy by decline in the first trimester miscarriage rate.

关键词： Vitamin D intracytoplasmic Sperm Injection ICSI

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Secondary sex ratio of assisted reproductive technology babies

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Asian pacific Journal of Reproduction 2019年第2期8卷 88-92页

作者： Nathira Abdul Majeed Charley Zheng Alex Polyakov Megan Pucci Mohamed Hatta Tarmizi Mie Mie Cho Win Royal Women's Hospital MelbourneVictoriaAustralia Royal Women's Hospital and Melbourne IVF MelbourneVictoriaAustralia Oceania University of Medicinea Australia Sabah Women's and Children's Hospital Kota KinabaluSabahMalaysia Obstetrics&Gynaecology Department International Medical SchoolManagement&Science UniversitySelangorMalaysia

Objective: To assess the secondary sex ratio (SSR) of assisted reproduction technology babies within a fertility clinic in Melbourne, Australia. Methods: A retrospective cohort study was conducted on 3369 babies who were born following single embryo transfer between 2011 and 2016. Variables examined included embryo creation date, maternal and paternal ages, maternal body mass index (BMI), type of infertility, sperm parameters, fertilization method, type of embryo and stage of transfer. Multivariate regression analysis was performed on the data set using STRATA V9.2. Results: More males were found to be born to embryos created in summer (adjusted odds ratio=1.46, 95% confidence interval: 1.08-1.95;P=0.01). Lower BMI of mother (≤30 kg/m 2) and younger age of parents (≤35 years) were associated with an increase in SSR (50.1% vs. 47.1%;50.6% vs. 48.8%;50.0% vs. 48.9%) respectively. Decreased SSR value was associated with primary subfertility, using in-vitro fertilization procedure and fresh embryo compared to those with secondary subfertility, using intracytoplasmic sperm injection procedure and thawed embryo (49.3% vs. 50.6%;47.1% vs. 50.8%;49.4% vs. 50.1%) respectively. Conclusions: The fertilization methods, type of embryo, stage of embryo transfer, parameters of the sperm and status of subfertility do not significantly affect the SSR. SSR is affected by the seasonality, maternal age, and BMI.

关键词： Artificial reproduction technology intracytoplasmic sperm injection In-vitro fertilization Secondary sex ratio

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Estimating zona pellucida hardness under microinjection to assess oocyte/embryo quality: Analytical and experimental studies

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Advances in Bioscience and Biotechnology 2013年第5期4卷 679-688页

作者： Morteza Khalilian Mojtaba Rezazadeh Valojerdi Mehdi Navidbakhsh Mahmoud Chizari Poopak Eftekhari-Yazdi Department of Anatomy Faculty of Medical sciences Tarbiat Modares University Tehran Iran Department of Embryology Reproductive Biomedicine Research Center Royan Institute for Reproductive Biomedicine Academic Center for Education Culture and Research (ACECR) Tehran Iran Mechanical Engineering Department Iran University of Science and Technology Tehran Iran School of Design and Engineering Brunel University West London London UK

The precise determination of zona pellucida (ZP) hardness is largely unknown due to the lack of appropriate measuring and modelling methods. In this study, we have used experimental and theoretical models to describe the mechanical behavior of a single oocyte cell to improve the assisted reproductive technology (ART) outcomes by assessing oocyte/embryo quality. This paper presents the development of: i) a microinjection model to estimate the force of ZP penetration, ii) a micropipette aspiration model to determine the corresponding hardness, and iii) an experimental procedure to generate the required data for these two models. Our results show that the estimated penetration force provides a performance target for the penetration process during intracytoplasmic sperm injection (ICSI), while the estimated corresponding hardness serves as an indicator of the extent of deformation sustained by the oocyte prior to penetration. Evaluation of these results shows that a routine assessment of ZP hardness under microinjection would allow for the identification of certain oocyte pools for which further manipulation is recommended in order to improve injection, hatching and finally ART outcomes.

关键词： Human Oocyte Human Embryo intracytoplasmic Sperm Injection Zona Pellucida Hardness

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