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Optogenetic activation of intracellular signaling based on light-inducible protein-protein homo-interactions

Neural Regeneration Research 2022年第1期17卷 25-30页

作者： Peiyuan Huang Zhihao Zhao Liting Duan Department of Biomedical Engineering The Chinese University of Hong KongSha TinHong Kong Special Administrative RegionChina Shun Hing Institute of Advanced Engineering(SHIAE) The Chinese University of Hong KongSha TinHong Kong Special Administrative RegionChina

Dynamic protein-protein interactions are essential for proper cell *** events—physical interactions between the same type of proteins—represent a pivotal subset of protein-protein interactions that are widely exploited in activating intracellular signaling *** of modulating protein-protein interactions with spatial and temporal resolution are greatly desired to decipher the dynamic nature of signal transduction *** emerging optogenetic technology,based on genetically encoded light-sensitive proteins,provides promising opportunities to dissect the highly complex signaling networks with unmatched specificity and spatiotemporal *** we review recent achievements in the development of optogenetic tools enabling light-inducible protein-protein homo-interactions and their applications in optical activation of signaling pathways.

关键词： cryptochrome 2 homo-interaction intracellular signaling light light-induced protein-protein interaction light-oxygen-voltage-sensing domain light-sensitive proteins optogenetics phytochrome signal transduction

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Clues to the signals for chloroplast photorelocation from the lifetimes of accumulation and avoidance responses

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Journal of Integrative Plant Biology 2015年第1期57卷 120-126页

作者： Takeshi Higa Masamitsu Wada Department of Biology Faculty of Sciences Kyushu University

Chloroplast photo-relocation movement is crucial for plant survival; however, the mechanism of this phenome- non is still poorly understood. Especially, the signal that goes from photoreceptor to chloroplast is unknown, although the photoreceptors （phototropin 1 and 2） have been identified and an actin structure （chloroplast actin filaments） has been characterized that is specific for chloroplast movement. Here, in gametophytes of the fern Adiantum capillus-veneris, gametophores of the moss Physcomiterella patens, and leaves of the seed plant Arabidopsis thaliana, we sought to characterize the signaling system by measuring the lifetime of the induced response. Chloroplast movements were induced by microbeam irradiation with high-intensity blue light and recorded. The lifetime of the avoidance state was measured as a lag time between switching off the beam and the loss of avoidance behavior, and that of the accumulation state was measured as the duration of accumulation behavior following the extinction of the beam. The lifetime for the avoidance response state is approximately 3-4rain and that for the accumulation response is 19-28 rain. These data suggest that the two responses are based on distinct signals.

关键词： Blue light chloroplast movement fern gametophyte intracellular signaling phototropin

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Function of Ca^(2+)-/calmodulin-dependent protein kinase Ⅳ in Ca^(2+)-stimulated neuronal signaling and behavior

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Science China(Life Sciences) 2015年第1期58卷 6-13页

作者： SONG ZiXiang CHEN Qi DING Qi ZHENG Fei LI ChengWei XU LePing WANG HongBing Department of Adolescent Psychiatry and Behavioral Therapy Heping Hospital Changzhou Department of Physiology Michigan State University Department of Biochemistry and Molecular Biology Michigan State University College of Life Science and Agronomy Zhoukou Normal University Neuroscience Program Michigan State University

The activity of Ca2+/calmodulin-dependent protein kinase IV(Ca MKIV) is sensitive to activity-dependent changes in the level of intracellular Ca2+.Following neuronal stimulation,the activation of Ca MKIV may trigger synaptic modifications and transcriptional responses,both of which are involved in regulating cognitive and emotional ***,we used Ca MKIV knockout(KO) neurons and mice to examine the function of Ca MKIV in Ca2+-stimulated intracellular signaling and animal behavior,*** NMDA receptor activation or membrane depolarization,the up-regulation of CREB(c AMP responsive element binding protein) and its target gene Bdnf(brain-derived neurotrophic factor) was intact in cortical neurons obtained from Ca MKIV KO *** MKIV KO mice displayed severe impairment in contextual fear memory but normal locomotor activity and anxiety level in the contextual training *** Ca MKIV KO mice showed normal memory in the standard passive avoidance task,they were defective in learning the temporal dissociative passive avoidance *** indicated by the light/dark test and marble-burying test data,Ca MKIV KO mice showed less anxiety and normal *** the voluntary wheel-running test,Ca MKIV KO mice showed normal running time and distance but higher maximal running *** results demonstrate the function of Ca MKIV in regulating different forms of fear memory,anxiety,and certain aspect of motor function.

关键词： animal behavior calmodulin CREB ERK1/2 intracellular signaling learning and memory

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