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An intracellular toxic protein (Xin) isolated from Xenorhabdus nematophilus strain BJ

Progress in Natural Science:Materials International 2002年第4期 72-74页

作者： PAN Yinghong , JIAN Heng , ZHANG Jie , LIU Zheng, CHEN Zhongyi , YANG Xiufen, YANG Huaiwen and HUANG Dafang( State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China Institute of Biocontrol Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China Institute of Biotechnology Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China.) State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100094 China Institute of Biocontrol Research Chinese Academy of Agricultural Sciences Beijing 100081 China Institute of Biotechnology Research Chinese Academy of Agricultural Sciences Beijing 100081 China

An intracellular toxic protein, designated Xin and with remarkable inhibitory effect on growth of Helicoverpa armiger-a, was isolated from X. nematophilus strain BJ by ion exchange chromatography and hydrophobic interaction chromatography. After placing neonates on the artificial diet containing Xin at concentration of 0 or 3.48 μg/g for 7 days, the average weight of insects was 23.50 mg and 0.55mg, respectively. The activity of Xin was lost by heating, freeze-drying, digestion with proteases or urea. The Xin molecular weight, over 1000 kD, was determined by gel filtration on Superose 12, and there were six component proteins analyzed by SDS-PAGE. The results show that Xin is a high molecular protein complex inhibiting growth of insect pest.

关键词： intracellular protein Helicoverpa armigera Xenorhabdus nematophilus Xin purification.

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Detection of lntraceUular proteins and Biomarkers Using Modified Silica Nanoparticles and Flow Cytometry In vitro

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Chemical Research in Chinese Universities 2018年第2期34卷 229-234页

作者： YU Yang MA Wei YU Lina ZHOU Shuyuan ZHU Guangshan SHI Wei Department of Sports and Health Guangzhou Institute of Physical Education Guangzhou 510500 P. R. China Key Laboratory for Molecular Enzymology and Engineering Ministry of Education College of Life Science Jilin Universily Changchun 130012 P. R. China China-Japan Union Hospital Jilin University Changchun 130033 P. R. China Key Laboratory of Oral Medicine Guangzhou Institute of Oral Disease Stomatology Hospital of Guangzhou Medical University Guangzhou 510140 P. R. China State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of Chemistry Jilin University Changchun 130012 R R. China Key Laboratory of Polyoxometalate Science Ministry of Education Faculty of Chemistry Northeast Normal University Changchun 130024 P. R. China

In this paper, antibody-modified silica nanoparticles were successfully synthesized, and their average di- ameter was （1094-9） nm. These particles were mixed with cell extracts to target a protein, then, an antibody labeled with fluorescein isothiocyanate（FITC） was added to form FITC-labeled nanoparticle complexes and the product was analyzed using flow cytometry. The results confirm that the intracellular proteins and biomarkers were precisely and sensitivity detected by the novel method in vitro. The FITC intensity of Akt antibody-conjugated particles was in- creased ≥ 10-fold compared with that of control samples in MCF-7 cells. Furthermore, it can also simultaneously measure several proteins when modified with different antibodies.

关键词： Silica nanoparticle Flow cytometry intracellular protein Biomarker

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