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Local hypothermia and optimal temperature for stroke therapy in rats

Chinese Medical Journal 2009年第13期122卷 1558-1563页

作者： WU Hao JIANG Li-dan Karsten H. Wrede JI Xun-ming ZHAO Xi-qing TIAN Xin GAO Yu-fei LING Feng Department of Neurosurgery Xuan Wu Hospital Capital Medical University Beijing 100053 China Departement of Neurosurgery International Neuroscience Institute Rudolf Pichlmayr Str. 4 30625 Hannover Germany Department of Neurosurgery the People Hospital of Tangshan Tangshan Hebei 063000 China Central Lab Xuan Wu Hospital Capital Medical University Beijing 100053 China Department of Neurosurgery China-Japan Union Hospital Ji Lin University Changchun Jilin 130033 China

Background Local hypothermia induced by intravascular infusion of cold saline solution effectively reduces brain damage in stroke. We further determined the optimal temperature of local hypothermia in our study. Methods Seventy-eight adult male Sprague Dawley rats （260-300 g） were randomly divided into 3 groups： group A, ischemia/reperfusion without cold saline infusion （n=26）（control group）; group B, infusion with 20℃ saline before reperfusion （n=26）; group C： infusion with 10℃ saline before reperfusion （n=26）. In each group, we chose 15 rats for monitoring physical indexes and the temperature of the brain （cortex and striatum） and body （anus）, measurement of brain infarction volume, assessment of neurological deficits and the survival rate of reperfusion at 48 hours. Another 8 rats from each group was chosen for examining brain edema, another 3 from each group for histological observation by electron microscopy （EM） and light microscopy （LM） at 48 hours after reperfusion. Results There was no significant difference among the 3 groups for physical indexes during the examination （F（2. 45）= 0.577, P=0.568; F（2.45）= 0.42, P=0.78 for blood pressure and blood gas analysis, respectively）. The brain temperature was significantly reduced in the group C compared to the other groups （F（2.45）=37.074, P=0.000; F（2.45）=32.983, P=0.000, for cortex and striatum temperature respectively）, while the difference in rectal temperature between group A and B or C after reperfusion was not significant （F（2.45）= 0.17115,P=0.637）. And the brain infarct volume was significantly reduced in group C （from 40%±10% in group A, 26%±8% in group B, to 12%±6% in group C, F（2.45）=43.465, P=0.000） with the neurological deficits improving in group C （Х^2=27.626, P=0.000）. The survival rate at 48 hours after 10℃ and 20℃ saline reperfusion was increased by 132.5% and 150%, respectively, as compared to the control group （Х^2=10.489, P=0.005）. The extent of the brain edema showed no significant difference （F（2.21）=0.547, P=0.587） after cold saline infu

关键词： rat middle cerebral artery occlusion intra-arterial hyperthermia ischemia/reperfusion injury

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