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Enhancement of innate immune system in monocot rice by transferring the dicotyledonous elongation factor Tu receptor EFR

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Journal of Integrative Plant Biology 2015年第7期57卷 641-652页

作者： Fen Lu Huiqin Wang Shanzhi Wang Wendi Jiang Changlin Shan Bin Li Jun Yang Shiyong Zhang Wenxian Sun Department of Plant Pathology China Agricultural University Key Laboratory in Plant Pathology Ministry of AgricultureChina Agricultural University State Key Laboratory of Rice Biology and Key Laboratory of Molecular Biology of Crop Pathogens and Insects Ministry of AgricultureInstitute of BiotechnologyZhejiang University Rice Research Institute Shandong Academy of Agricultural Science

The elongation factor Tu （EF-Tu） receptor （EFR） in cruciferous plants specifically recognizes the N-terminal acetylated elf18 region of bacterial EF-Tu and thereby activates plant immunity. It has been demonstrated that Arabidopsis EFR confers broad-spectrum bacterial resistance in the EFR transgenic solanaceous plants. Here, the transgenic rice plants （Oryza sativa L. ssp. japonica cv. Zhonghua 17） and cell cultures with constitutive expression of AtEFR were developed to investigate whether AtEFR senses EF-Tu and thus enhances bacterial resistance in the monocot plants. We＇demonstrated that the Xanthomonas oryzae-derived elf18 peptide induced oxidative burst and mitogen-activated protein kinase activa- tion in the AtEFR transgenic rice cells and plants, respectively. Pathogenesis-related genes, such as OsPBZ1, were upregulated dramatically in transgenic rice plant and cell lines in response to elf18 stimulation. Importantly, pretreatment with elf18 trig- gered strong resistance to X. oryzae pv. oryzae in the transgenic plants, which was largely dependent on the AtEFR expressionlevel. These plants also exhibited enhanced resistance to rice bacterial brown stripe, but not to rice fungal blast. Collectively, the results indicate that the rice plants with heterologous expression of AtEFR recognize bacterial EF-Tu and exhibit enhanced broad-spectrum bacterial disease resistance and that pattern recognition receptor-mediated immunity may be manipulated across the two plant classes, dicots and monocots.

关键词： Bacterial disease resistance elongation factor Tu receptor interspecific gene transfer pattern recognition receptor rice

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