检索结果-南通市图书馆

Cell Research 2003年第5期13卷 385-391页

作者： JIAN WEN DONG, HAI FENG ZHU, WEI ZHONG ZHU, HAI LEI DING, TIE MIN MA, ZHAO NIAN ZHOU 1 Laboratory of Hypoxic Cardiovascular Physiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.2 Department of Physiology and Pathophysiology, Health Science Center Peking University, Beijing 100083 China Laboratory of Hypoxic Cardiovascular Physiology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai China Department of Physiology and Pathophysiology Health Science Center Peking University Beijing China

intermittent hypoxia has been shown to provide myocardial protection against ishemia/reperfusion-induced injury. Cardiac myocyte loss through apoptosis has been reported in ischemia/reperfusion injury. Our aim was to investigate whether intermittent hypoxia could attenuate ischemia/reperfusion-induced apoptosis in cardiac myocytes and its potential mechanisms. Adult male Sprague-Dawley rats were exposed to hypoxia simulated 5000 m in a hypobaric chamber for 6 h/day, lasting 42 days. Normoxia group rats were kept under normoxic conditions. Isolated perfused hearts from both groups were subjected to 30 min of global ischemia followed by 60 min reperfusion. Incidence of apoptosis in cardiac myocytes was determined by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling （TUNEL） and DNA agarose gel electrophoresis. Expressions of apoptosis related proteins, Bax and Bcl-2, in cytosolic and membrane fraction were detected by Western Blotting. After ischemia/reperfusion, enhanced recovery of ca

关键词： intermittent hypoxia apoptosis cardiac myocytes Bax Bcl-2.

intermittent hypoxia is involved in gut microbial dysbiosis in type 2 diabetes mellitus and obstructive sleep apnea-hypopnea syndrome

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2022年第21期28卷 2320-2333页

作者： Sha-Sha Tang Cheng-Hong Liang Ya-Lei Liu Wei Wei Xin-Ru Deng Xiao-Yang Shi Li-Min Wang Li-Jun Zhang Hui-Juan Yuan Department of Endocrinology Henan Provincial Key Medicine Laboratory of Intestinal Microecology and DiabetesHenan Provincial People’s HospitalPeople’s Hospital of Zhengzhou UniversityZhengzhou 450003Henan ProvinceChina

BACKGROUND Obstructive sleep apnea(OSA)-hypopnea syndrome(OSAHS)has been recognized as a comorbidity of type 2 diabetes mellitus(T2DM);more than half of T2DM patients suffer from *** hypoxia(IH)plays an important role in metabolic diseases,such as obesity and OSAHS,through various mechanisms,including altering the gut microecological composition and ***,it is important to study the role of gut microbiota in T2DM patients with OSAHS,which has a high incidence and is prone to several *** To assess whether IH is involved in altering the fecal microbiome in T2DM patients with *** Seventy-eight participants were enrolled from Henan Province People’s Hospital and divided into healthy control(HC,n=26),T2DM(n=25),and T2DM+OSA(n=27)groups based on their *** fecal bacterial DNA of the research participants was extracted and subjected to 16S ribosomal RNA *** clinical indices,such as insulin resistance index,homocysteine(HCY)concentration,and the concentrations of inflammatory factors in the peripheral blood,were assessed and *** Group T2DM+OSA had the highest apnea-hypopnea index(AHI)(2.3 vs 3.7 vs 13.7),oxygen desaturation index(0.65 vs 2.2 vs 9.1),HCY concentration(9.6μmol/L vs 10.3μmol/L vs 13.81μmol/L)and C-reactive protein(CRP)concentrations(0.3 mg/L vs 1.43 mg/L vs 2.11 mg/L),and lowest mean oxygen saturation(97.05%vs 96.6%vs 94.7%)among the three *** and fifteen key differences in amplicon sequence variants were identified when comparing group T2DM+OSA with groups T2DM and HC,*** found progressively decreased levels of Faecalibacterium,Eubacterium,and Lachnospiraceae,and an increase in the level of Actinomyces,which strongly correlated with the HCY,CRP,fasting plasma glucose,and hemoglobin A1c concentrations,AHI,mean oxygen saturation,and insulin resistance index in group T2DM+OSA(P<0.05).CONCLUSION For T2DM patients with OSAHS,IH may be involved in selective

关键词： Gut microbiota Obstructive sleep apnea-hypopnea syndrome Type 2 diabetes mellitus intermittent hypoxia Obstructive sleep apnea

Inducible nitric oxide synthase contributes to intermittent hypoxia against ischemia/reperfusion injury

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2005年第3期26卷 315-322页

作者： Hai-lei DING~2 Hai-feng ZHU~2 Jian-wen DONG~2 Wei-zhong ZHU~2 Wei-wei YANG~3 Huang-tian YANG~3 Zhao-nian ZHOU~(2,4)2 Physiological Laboratory of hypoxia,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China 3 Laboratory of Molecular Cardiology,Health Science Center,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences and Shanghai Second Medical University,Shanghai 200025,China Physiological Laboratory of hypoxia Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Laboratory of Molecular Cardiology Health Science Center Shanghai Institutes for Biological Sciences Chinese Academy of Sciences and Shanghai Second Medical University Shanghai China

Aim:To investigate the role of inducible nitric oxide synthase (iNOS)-derivednitric oxide (NO) in the cardioprotection of intermittent hypoxia (IH) against is-chemia/reperfusion (I/R) ***:Langendorff-perfused isolated rathearts were used to measure variables of left ventricular function during baselineperfusion,ischemia,and reperfusion *** plus nitrite (NOx) content inmyocardium was measured using a biochemical method,iNOS mRNA and pro-tein expression in rat left ventricles were detected using reverse transcription poly-merase chain reaction (RT-PCR) and Western blot,***:Myo-cardial function recovered better in IH rat hearts than in normoxic control *** iNOS-selective inhibitor aminoguanidine (AG) (100μmol/L) significantlyinhibited the protective effects of IH,but had no influence on normoxic rat *** baseline content of NOx in IH hearts was higher than that in normoxic *** 30 min ischemia,the NOx level in normoxic hearts increased compared tothe corresponding baseline level,whereas there was no significant change in ***,the NOx level in IH hearts was still higher than that of normoxichearts during ischemia and reperfusion *** 100μmol/L significantly di-minished the NOx content in IH and normoxic hearts during ischemia and *** baseline levels of iNOS mRNA and protein in IH hearts were higherthan those of normoxic *** to the corresponding baseline level,iNOS mRNA and protein levels in normoxic rat hearts increased and those in IH rathearts decreased after *** addition of AG 100μmol/L significantlydecreased iNOS mRNA and protein expression in IH rat hearts after I/***:IH upregulated the baseline level of iNOS mRNA and protein expres-sion leading to an increase in NO production,which may play an important role inthe cardiac protection of IH against I/R injury.

关键词： intermittent hypoxia ischemia/reperfusion inducible nitric oxide synthase aminoguanidine: rats

intermittent hypoxia with or without hypercapnia is associated with tumorigenesis by decreasing the expression of brain derived neurotrophic factor and miR-34a in rats

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Journal 2014年第1期127卷 43-47页

作者： Zhang Jing Guo Xu Shi Yanwei Ma Jing Wang Guangfa Department of Respiratory and Critical Care Medicine PekingUniversity First Hospital Beijing 100034 China Department of Neurosurgery Liaoning Cancer Hospital & InstituteShenyang Liaoning 110042 China Department of RespiratoryMedicine Peking University ShougangHospital Beijing 100041 China

Background Very recent studies revealed that obstructive sleep apnoea （OSA） is a contributor of the increased incidence and mortality of cancer in humans,but mechanisms of how OSA promotes tumorigenesis remains largely *** investigated whether intermittent hypoxia with and without hypercapnia plays a role in *** First,Sprague-Dawley （SD） male rats （12 weeks old） were subjected to different hypoxia exposures：intermittent hypoxia and intermittent hypoxia with hypercapnia; continuous hypoxia and normal *** systemic application of chronic fast rate hypoxia with or without hypercapnia mimicked severe OSA patients with apnoea/hypopnea index equivalent to 60 events per *** routine blood tests were performed and the levels of brain derived neurotrophic factor （BDNF） and miR-34a were *** In contrast to intermittent hypoxia with hypercapnia,both intermittent hypoxia and continuous hypoxia treatments caused significantly higher levels of haematology parameters than normoxia *** to normoxia,intermittent hypoxia with hypercapnia exposure resulted in substantial decrease of serum BDNF and,miR-34a in the lower brainstem,while less pronounced results were found in intermittent hypoxia and continuous hypoxia *** The exposure of intermittent hypoxia with or without hypercapnia,mimicking the situations in severe OSA patients,was associated with,or even promoted tumorigenesis.

关键词： intermittent hypoxia hypercapnia sleep apnoea tumorigenesis brain derived neurotrophic factor miR-34a

维普期刊数据库

intermittent hypoxia reduces microglia proliferation and induces DNA damage in vitro

在线全文

学校读者我要写书评

暂无评论

Intermittent hypoxia reduces microglia proliferation and ind...

intermittent hypoxia promotes neurogenesis in dentate gyrus of hippocampus mediated by Notch1

中国睡眠研究会第九届学术年会

作者：刘松陈葵徐波孙晋渊任连萍上海交通大学医学院附属新华医院呼吸科

＜正＞Background:Obstructive sleep apnea（OSA）is a common sleep disorder characterized by recurrent episodes of complete or partial collapse of the upper airway,resulting in nocturnal intermittent hypoxia（IH）.This disease has many potential consequences including neurocognitive and behavioral ***,caused by OSA,could cause hippocampus or neuron damage through multiple signaling pathways,while the underlying mechanisms are still unclear.

关键词： intermittent hypoxia microglia p53 signaling pathways inflammatory cytokine

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Intermittent hypoxia promotes neurogenesis in dentate gyrus ...

Hepatic Cyp1a2 Expression Reduction during Inflammation Elicited in a Rat Model of intermittent hypoxia

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Kuan ZHANG~1,Tong ZHAO~1,Xin HUANG~1,Jia SUN~1,Yun-Ling HE~1,Li-Ying WU~1,Ling-Ling ZHU~(1,*),Ming FAN~(1,*) 1 Department of Brain protection and Plasticity,Institute of Basic Medical Sciences,No.27 Taiping Rd,Beijing 100850, China

＜正＞Objective Increasing evidence indicates that in vitro neurogenesis is enhanced by hypoxia,and recent studies have shown that Notch1 signaling pathway,a classic pathway regulating neurogenesis,can be activated by *** aim of this study was to investigate whether neurogenesis in dentate gyrus（DG） of hippocampus can be promoted by intermittent hypoxia（IH） and the role of Notchl in the hippocampal neurogenesis enhanced by IH. Methods The proliferation of neural stem cells（NSCs） was identified by Brdu *** differentiation and migration of NSCs were identified by Brdu and NeuN double *** morphology of astrocyte was identified by GFAP immunohistochemistry *** 1-transgenic mice were used to identified the structure of *** deficiency mice were used to identified the role of ***（1） intermittent hypoxia could promote the neurogenesis in DG of hippocampus,including proliferation of NSCs,migration of neuron,development of spine and astrocyte.（2） The promoted effects of intermittent hypoxia on neurogenesis were inhibited in Notchl deficiency *** intermittent hypoxia could promote the neurognesis in DG,which was mediated through Notch 1 signaling pathway.

关键词： intermittent hypoxia dentate gyrus neurogenesis Notch1

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Chinese Medical Journal 2017年第21期130卷 2585-2590页

作者： Li-Xia Shi Xing Wang Qi Wu Xin Sun Zhen Wan Li Li Kuan Li Xue Li Yu Li Qiu-Yang Zhang Jun-Ping Wu Huai-Yong Chen Department of Respiratory Medicine Haihe Clinical College of Tianjin Medical University Tianjin 300350 China Key Research Laboratory for Infectious Disease Prevention for State Administration of Traditional Chinese Medicine Tianjin Haihe Hospital Tianjin 300350 China Department of Basic Medicine Tianjin Institute of Respiratory Diseases Tianjin Haihe Hospital Tianjin 300350 China

Background： intermittent hypoxia （IH） is a key element of obstructive sleep apnea （OSA） that can lead to disorders in the liver. In this study, IH was established in a rat model to examine its effects on the expression of hepatic cytochrome P450 （CYP） and CYP regulators, including nuclear receptors. Methods： Hematoxylin and eosin staining was conducted to analyze the general pathology of the liver of rats exposed to IH. The messenger RNA （mRNA） expression levels of inflammatory cytokines, CYPs, nuclear factor-κB （NF-κB）, and nuclear factors in the liver were measured by quantitative reverse transcription polymerase chain reaction. Results： We found inflammatory infiltrates in the liver of rats exposed to IH. The mRNA expression level of interleukin-1beta was increased in the liver of the IH-exposed rats （0.005 ± 0.001 vs. 0.038 ± 0.008, P = 0.042）, whereas the mRNA expression level of Cyp1a2 was downregulated （0.022 ± 0.002 vs. 0.0050 ± 0.0002, P = 0.029）. The hepatic level of transcription factor NF-κB was also reduced in the IH group relative to that in the control group, but the difference was not statistically significant and was parallel to the expression of the pregnane X receptor and constitutive androstane receptor. However, the decreased expression of the glucocorticoid receptor upon IH treatment was statistically significant （0.056 ± 0.012 vs. 0.032 ± 0.005, P = 0.035）. Conclusions： These results indicate a decrease in expression of hepatic CYPs and their regulator GR in rats exposed to IH. Therefore, this should be noted for patients on medication, especially those on drugs metabolized via the hepatic system, and close attention should be paid to the liver function of patients with OSA-associated IH.

关键词： Cytochrome P450 Glucocorticoid Receptor Inflammation intermittent hypoxia Nuclear Factor-kB

维普期刊数据库

intermittent hypoxia enhances the tumor programmed death ligand 1 expression in mice

在线全文

学校读者我要写书评

暂无评论

Intermittent hypoxia enhances the tumor programmed death lig...

Ang II type 1 receptor expression in rat aorta exposed to chronic intermittent hypoxia： effects of p38MAPK and ERK1/2 signaling

中国睡眠研究会第十届全国学术年会

作者： Xiao-Bin Zhang Liu-Xia Li Yi-Ming Zeng Chih-Yuan Ko Yan-Ping Du Miao Wang Hui-Qing Zeng Xiongbiao Luo Department of Respiratory Medicine Zhongshan HospitalXiamen University Department of Respiratory and Critical Care Medicine the Second Affiliated Hospital of Fujian Medical Universitythe Second Clinical Medical College of Fujian Medical UniversityCenter of Respiratory Medicine of Fujian Province Computer College Xiamen University

Objective: As a pathophysiological hallmark of obstructive sleep apnea（OSA）,intermittent hypoxia（IH） promotes the tumor *** high expression of programmed death 1 and programmed death ligand 1（PDL-1） in tumor leads to immune evasion,subsequently aggravates tumor *** study aims to determine the tumor PDL-1 expression under IH *** and methods: A total of 24 male C57 BL/6 J mice were randomly assigned to the normoxia（control,CTL） group and the IH *** in IH group were subjected to IH condition for 5 *** Lewis lung cells were injected into the flank of each mice after 1 weeks of IH *** weight,tumor volume,and tumor weight were *** blotting was conducted to assess the hypoxia inducible factor 1?（HIF-1 ?） and PDL-1 in tumor tissue between the two *** PDL-1 expression were also detected by immunohistochemistry（IHC）.Results: Mice in IH group had low body weight,high tumor volume and tumor *** blotting results showed higher tumor HIF-1? and PDL-1 expression levels in mice under the IH condition than under the CTL *** IHC results also confirmed that compared to the CTL group,mice in the IH group had high PDL-1 ***: IH can enhance the tumor PDL-1 expression,we speculate that high PDL-1 levels is associated with poor outcome in patients with OSA and *** results of the present provided experiment evidence for using anti-PD-1/PDL-1 antibodies to treat cancer among OSA patients.

关键词： intermittent hypoxia programmed death ligand 1 tumor sleep apnea

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Chinese Medical Journal 2013年第17期126卷 3264-3269页

作者： SHANG Jin YANG Yuan-yuan GUO Xue-ling LIU Hui-guo Department of Respiratory and Critical Care Medicine Tongji Hospital Huazhong University of Science and Technology Wuhan Hubei 430030 China

Background Obstructive sleep apnea is a frequent medical condition consisting of repetitive sleep-related episodes of upper air ways obstruction and can lead to hypertension. Ang II type 1 receptor （AT1R） played important roles in hypertension since it binds with Ang II, controlling salt-water and blood pressure homeostasis. This study explores rat aorta AT1R expression during intermittent hypoxia （IH） and the signaling pathways involved. Methods A rat model and a cell model used a BioSpherix-OxyCycler A84 system and a ProOx C21 system respectively. The arterial blood pressure was recorded by a Nihon Kohden Polygraph System. Immunohistochemic was used to focus and analyze the expression of AT1R in rat aorta. Real-time PCR and Western blotting were used to explore the signaling pathways that participated in AT1R expression. Results In this study, we found that chronic intermittent hypoxia （CIH） induced AT1R transcription which increased the blood pressure in rat aorta compared to normoxia and to sustained hypoxia. The AT1R protein expression in the aorta was similar to the real-time PCR results. We explored the signaling mechanisms involved in the AT1R induction in both rat aorta and the aortic endothelial cells by real-time PCR and Western blotting. Compared to normoxia, CIH increased ERK1 mRNA transcription but not ERK2 or p38MAPK in the aorta; whereas sustained hypoxia （SH） upregulated ERK2 but not ERK1 or p38MAPK mRNA. In cells, IH induced AT1R expression with ERK1/2 phosphorylation but reduced p38MAPKs phosphorylation, whereas SH induced only ERK1/2 phosphorylation. The ERK1/2 inhibitor PD98059 attenuated the IH- induced AT1R increase but the p38MAPK inhibitor SB203580 did not. Conclusions Our results indicate that CIH induced the elevation of rat blood pressure and aorta AT1R expression. Moreover, ATIR expression in IH and sustained hypoxia might be regulated by different signal transduction pathways, highlighting a novel regulatory function through ERK1/2 sign

关键词： intermittent hypoxia hypertension Ang II type 1 receptor ERK1/2 p38MAPK

Adiponectin protects the genioglossus of rats against chronic intermittent hypoxia-induced injury via inhibition of endoplasmic reticulum stress

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Journal 2013年第17期126卷 3270-3275页

作者： ZHANG Xiao-feng HUANG Han-peng DING Wen-xiao DING Ning LU Gan LIU Jian-nan ZHANG Xi-long Department of Respiratory Diseases First Affiliated Hospital of Nanjing Medical University Nanjing Jiangsu 210029 China Department of Respiratory Diseases Jiangsu Geriatric Hospital Nanjing Jiangsu 210024 China

Background Obstructive sleep apnea hypopnea syndrome, characterized by chronic intermittent hypoxia （CIH）, is closely correlated with genioglossus dysfunction. CIH has been identified to mediate mitochondrial damage in genioglossus. It has been reported that endoplasmic reticulum stress （ERS） could be induced by mitochondrial dysfunction. This study aimed to investigate the role of ERS in CIH-induced genioglossus injury, as well as the possible intervention effect of adiponectin （Ad） supplement in rats. Methods Forty-five male Wistar rats were randomly divided into three groups and submitted to room air （group A, n=15） as a control or CIH （groups B and C, n=15, respectively）. Throughout the exposure period, intravenous Ad was given in group C; while intravenous normal saline was simultaneously given in groups A and B. After 35-day exposure, genioglossus samples were obtained from the pentobarbital-anaesthetized rats via surgical dissection, following blood sampling. Western blotting was applied to detect expressions of ERS signals and associated apoptotic pathways in genioglossus. Serum adiponectin levels were assessed via enzyme-linked immunosorbent assay （ELISA）. Results Significant hypoadiponectinemia was revealed in group B only （P 〈0.05）. Compared to those in groups A and C, expressions of markers involved in ERS, such as glucose regulated protein 78 （GRP78）, p-PERK, phosphorylated eukaryotic initiation factor 2α （p-elF2α）, phosphorylated inositol-requiring transmembrane kinase/endoribonuclease 1α （p-IRE1α）, spliced X-Box binding protein 1 （XBPls） and activating transcription factor 6 （ATF6）, were significantly enhanced in group B （all P 〈0.01）; while no significant difference was shown between groups A and C （all P 〉0.05）. ERS- associated apoptotic pathways were remarkably activated in group B. The involved markers detected as the expression of CCAAT/enhancer binding protein homologous protein （CHOP）, B-cell lymphoma/leukemia associatied X protein （BAX） and ca

关键词： obstructive sleep apnea hypopnea syndrome intermittent hypoxia genioglossus endoplasmic reticulum stress adiponectin

维普期刊数据库