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IL-36β Promotes Inflammatory Activity and Inhibits Differentiation of Keratinocytes In Vitro

Chinese Medical Sciences Journal 2019年第3期34卷 199-204页

作者：王文明吴超余晓玲晋红中中国医学科学院北京协和医学院北京协和医院皮肤科南方医科大学皮肤病医院皮肤科

Objective Psoriasis is an immune-mediated inflammatory *** advances in the study of its pathogenesis,the exact development mechanism of psoriasis remains to be fully *** epidermis plays a crucial role in *** study aimed to investigate the effects of interleukin-36β(IL-36β)on keratinocyte dysfunction in *** Human keratinocyte cell lines,HaCaT cells,were treated with 0(control),50 or 100 ng/ml IL-36βrespectively for 24 *** viability was determined with a cell counting kit-8 *** cytometry was used to assess the effects of IL-36βon apoptosis and cell cycle *** of the differentiation markers,such as keratin 10 and involucrin,were evaluated by quantitative real-time polymerase chain reaction(RT-qPCR).Expressions of the inflammatory cytokines,IL-1βand IL-6 were tested by *** CCK8 assay showed the survival rate had no significant difference between the control and treated group(P>0.05).Flow cytometry analysis showed cell cycle arrest at S phase in the IL-36β-treated groups compared with the control group(P36β-treated groups compared with the negative control(P36βenhanced levels of IL-1βand IL-6 in culture supernatants of HaCaT cells compared with the negative control(P36βcould induce cell cycle arrest at S phase,inhibit keratin 10 and involucrin expressions and promote inflammatory activity in HaCaT cell lines.

关键词： interleukin-36β psoriasis keratinocytes inflammatory activity differentiation

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Function and mechanism of IL-36β on promoting antitumor immune response through activating CD8+T cells

Function and mechanism of IL-36β on promoting antitumor imm...

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第十届全国免疫学学术大会

作者： Xuefeng Wang Xin Zhao Yibei Zhu Xueguang Zhang Binfeng Lu Department of Biochemistry and Molecular Biology School of Biology and Basic Medical SciencesSoochow University Department of Immunology University of Pittsburgh School of Medicine 200 Lothrop Street Department of Immunology Institute of Medical Biotechnology School of Biology and Basic Medical Sciences Soochow University Department of General Surgery The First Affiliated Hospital of Soochow University

Background:Cytokines play a pivotal role in breaking immune tolerance of tumor antigens and increase response rates to ***-36/IL-36R signal has been demonstrated to have an effective function on promoting Th1 immune ***,the impact of IL-36βon tumor immunity is not ***:In the current study,we aim to reveal the function and mechanism of IL-36βin promoting CD8+T cell activation as well as antitumor immune ***:In vitro,the activation,proliferation and cytokine production of CD8+cells were determined by using flow cytometry,CFSE labeling,cell counting and ELISA assaying *** cytometry was also adopted to analyze the infiltrating *** data were analyzed with Graph Pad 6.0 software Result:It was found that IL-36βpromoted nave CD8+T cell activation and expansion at early ***,IL-36βcould stimulate m TORC1 activation dependent on My D88 and IKK ***,IL-36βwas identified to inhibit tumor progression,transformed the immunogenic tumor microenvironment and enhanced tumor antigen-specific CD8+T cell ***:Here we reveal the function and mechanism of IL-36βin promoting CD8+activation and ***,our work shows IL-36βcan break immune tolerance against tumor antigens.

关键词： interleukin-36β tumor microenvironment CD8+T cells m TORC1 My D88

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