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PRODUCTION AND CYTOGENETIC STUDY OF intergeneric HYBRIDS BETWEEN Triticum aestivum AND Agropyron desertorum

Science China Chemistry 1991年第4期34卷 421-428页

作者：李立会董玉琛 Institute of Crop Germplasm Resources Chinese Academy of Agricultural Sciences Beijing 100081 PRC

Through embryo culture, intergeneric hybrids were produced between common wheat,Triticum aestivum cv. Chinese Spring (CS) (2n= 6x = 42, AABBDD) and crested wheatgrass,Agropyron desertorum (2n =4x =28, PPPP) for the first time. Their F_2 and BC_1 seeds havealso been successfully obtained by selfing and backcrossing with wheat in F_1 hybrids. Theseedlings of all F_1 hybrids tended to resemble their female parent, but they had more tillers,and their spike characteristics were between their parents. Average meiotic chromosomepairing at MI of F_1 hybrids was: 6.62Ⅰ+ 8.20RingⅡ+ 4.16RodⅡ+0.57Ⅲ+ 0.35Ⅳ+0.06Ⅴ+0.03Ⅵ. The successful experience of hybridization of common wheat with A. desertorum,the reason for the high level of chromosome pairing and self-fertility in the F_1 hybrids werediscussed. The F_2 and BC_1 seeds obtained in F_1 hybrids are of great importance to theory andpractice.

关键词： Triticum aestivum Agropyron desertorum intergeneric hybrid chromosome pairing chromosome promotion

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FIRST intergeneric HYBRID BETWEEN Triticum aestivum AND Psathyrostachys juncea

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Chinese Science Bulletin 1988年第24期33卷 2071-2074页

作者：陈勤周荣华李立会李秀全杨欣明董玉琛 Institute of Crop Germplasra Resources Chinese Academy of Agricultural Sciences Beijing

Introduction In order to improve cultivated wheat, a lot of intergeneric hybrids between wheat and its relatives, such as Aegilops, Secale, Haynaldia, Hordeum, Thinopyrum, Pseudoroegneria, Elytrigia, Elymus and Leymus... 详细信息

关键词： Triticum aestivum Psathyrostaceys juncea intergeneric hybrid chromosome number

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High efficiency production and genomic in situ hybridization analysis of Brassica aneuploids and homozygous plants

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Science China(Life Sciences) 2003年第1期46卷 104-112页

作者：李再云 M.Ceccarelli S.Minelli A.Contento 刘焰 P.G.Cionini National Key Lab of Crop Genetic Improvement Department of AgronomyHuazhong Agricultural University Department of Cellular and Molecular Biology University of Perugia

Interspecific and intergeneric hybridizations have been widely used in plant genetics and breeding to construct stocks for genetic analysis and to introduce into crops the desirable traits and genes from their relatives. The intergeneric crosses between Brassica juncea (L.) Czern. & Coss., B. carinata A. Braun and Orychophragmus violaceus (L.) O. E. Schulz were made and the plants produced were subjected to genomic in situ hybridization analysis. The mixoploids from the cross with B. juncea were divided into three groups. The partially fertile mixoploids in the first group (2n = 36—42) mainly contained the somatic cells and pollen mother cells (PMCs) with the 36 chromosomes of B. juncea and additional chromosomes of O. violaceus. The mixoploids (2n = 30—36) in the second and third groups were morphologically quite similar to the mother plants B. juncea and showed nearly normal fertility. The plants in the second group produced the majority of PMCs (2n = 36) with their chromosomes paired and segregated normally, but 1—4 pairs of the O. violaceus chromosomes were included in some PMCs. The plants in the third group produced only PMCs with the 36 B. juncea chromosomes, which were paired and segregated normally. The mixoploids (2n = 29—34) from the cross with B. carinata produced the majority of PMCs (2n = 34) with normal chromosome pairing and segregation, but some plants had some PMCs with 1—3 pairs of chromosomes from O. violaceus and other plants had only PMCs with the B. carinata chromosomes. The Brassica homozygous plants and aneuploids with complete or partial chromo-some complements of Brassica parents and various numbers of O. violaceus chromosomes were derived from these progeny plants. The results in this study provided the molecular cytogenetic evidence for the separation of parental genomes which was previously proposed to occur in the hybridizations of these two genera.

关键词： Brassica species, Orychophragmus violaceus, aneuploids, intergeneric hybrids, genome separation.

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Developing Stable Cultivar through Microspore Mutagenesis in ×Brassicoraphanus koranhort, Inter-Generic Allopolyploid between Brassica rapaand Raphanus sativus

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American Journal of Plant Sciences 2017年第6期8卷 1345-1356页

作者： Soo-Seong Lee Byoung Ho Hwang Tae Yoon Kim Jeongmin Yang Na Rae Han Jongkee Kim Hyun Hee Kim Hadassah Roa Belandres BioBreeding Institute Sillyeong-Ro 153 Daeduk Ansung Gyeonggi Korea Dept. of Integrative Plant Science Chung-Ang University Ansung Korea Plant Biotechnology Institute Department of Life Science Sahmyook University Seoul Korea

A stable progeny was developed through induced mutation, using microspore culture, of the hybrid (F1F1) produced by crossing a newly synthesized, unstable allopolyploid (F1) and a stable cultivar, BB#1(F1) in xBrassicoraphanus. An F1F1 plant was subjected to the induced mutation system established during production of BB#1. Morphological characteristics of the progeny such as color, and leaf number and length, differed from those of BB#1. The bolting time of the progeny in spring cropping was very late compared to BB#1, allowing it to be grown to an adult plant in spring. Genomic in situ hybridization analysis of pollen mother cells at prophase I identified 19 bivalents, 10 from Brassica rapa and 9 from Raphanus sativus. The glucoraphenin content was almost identical to that of BB#1. Two cultivars are available in the baemoochae crop now. These results indicate that induced mutation using microspore culture is a viable method of stabilizing intergeneric allopolyploids between B. rapa and R. sativus.

关键词： xBrassicoraphanus intergeneric Hybrid Baemoochae Brassica rapa Raphanus sativus

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