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Effects of hepatitis E virus infection on interferon production via ISG15

World Journal of Gastroenterology 2018年第20期24卷 2173-2180页

作者： Min Wang Ying Huang Man He Wen-Ju Peng De-Ying Tian Tongji Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030Hubei ProvinceChina The First Affiliated Hospital of Sun Yat-sen University Guangzhou 510700Guangdong ProvinceChina

AIM To assess the effects of hepatitis E virus(HEV) on the production of type Ⅰ interferons(IFNs) and determine the underlying *** We measured the production of interferon(IFN)-alpha and-beta(-α/β) in genotype 3 HEV-infected C3 A cells at different time points(0, 8, 12, 24, 48, 72 and 120 h) by enzyme-linked immunosorbent assay(ELISA). The expression levels of IFN-stimulated gene(ISG)15 in HEVinfected C3A cells at different time points were tested by western blotting. The plasmid-expressing open reading frame 3(ORF3) or control plasmids(green fluorescent protein-expressing) were transfected into C3A cells, and the levels of IFN-α/β and ISG15 were evaluated, respectively. Furthermore, the plasmid-expressing ISG15 or small interfering RNA-inhibiting ISG15 was transfected into infected C3A cells. Then, the production of IFN-α/β was also measured by *** We showed that genotype 3 HEV could enhance the production of IFN-α/β and induce elevation of ISG15 in C3A cells. HEV ORF3 protein could enhance the production of IFN-α/β and the expression of ISG15. Additionally, ISG15 silencing enhanced the production of IFN-α/β. Overexpression of ISG15 resulted in the reduction of IFN-α/β.CONCLUSION HEV may promote production of IFN-α/β and expression of ISG15 via ORF3 in the early stages, and increased ISG15 subsequently inhibited the production of IFN-α/β.

关键词： open reading frame 3 interferon-stimulated gene 15 interferon-alpha Hepatitis E virus interferonbeta

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