检索结果-南通市图书馆

Antagonist peptides of human interferon-α2b isolated from phage display library inhibit interferon induced antiviral activity

引用

Acta Pharmacologica Sinica 2006年第8期27卷 1044-1050页

作者： Wang TIAN~1 Gang bAI~(2,3) Zheng-he LI~2 Wen-bo YANG~2 ~1Department of Microbiology,College of Life Sciences,Nankai University,Tianjin 300071,China," 2College of Life Sciences,Nankai University,Tianjin 300071,China Department of Microbiology College of Life Sciences Nankai University Tianjin China College of Life Sciences Nankai University Tianjin China

Aim:To screen human interferon(IFN)-α2b antagonist peptides from a phagedisplayed heptapeptide ***:WISH cells and polyclonal anti-IFN-α2b antibodies were used to select IFN receptor-binding peptides from a phagedisplayed heptapeptide *** specific binding of phage clones was exam-ined by phage ELISA and *** specific binding activitiesof synthetic peptides to WISH cells were detected by competition *** synthetic peptides to IFN-induced antiviral activity were analyzed by evaluat-ing the cytopathic effect(CPE)using the MTT ***:Twenty-threepositive clones were obtained after seven rounds of *** clones wererandomly picked from the positive clones and were *** correspond-ing amino acid sequences suggested 3 groups homologous to the 3 domains ofIFN-α2b,defined by residues 24-41,43-49,and 148-158 of IFN-α*** theypresented as corresponding to IFN receptor-binding domains,Ab loop and Ehelix,clone № 26 and 35 were chosen for further characterization and shown tobind to WISH *** peptides corresponding to clone № 26 and 35,desig-nated SP-7(SLSPGLP)and FY-7(FSAPVRY)were shown to compete with GFP-IFN-α2b for binding to its receptor and to inhibit the IFN-α2b-induced ***:both IFN-α2b antagonist peptides,SP-7 and FY-7,wereable to inhibit the IFN-induced antiviral activity,and could be helpful in laying thefoundation for the molecular mechanism of the interaction between IFN and itsreceptor.

关键词： interferon-α2b antagonist peptide phage display type Ⅰ IFN receptor cell selection

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：