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Effects of a Single and Low Dose of Mifepristone on integrin Expression in Human Endometrium During the Peri-implantation Period

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Journal of Reproduction and Contraception 2002年第4期13卷 214-221页

作者： Jie WU Jie-dong WANG Long-sheng WANG Peng-di ZHU Jie CHENG Department of Histology and Embryology Medical School Xi''an Jiaotong University Xi''an 710081 China National Research Institute for Family Planning Beijin 100081 China Department of Histology and Embryology Medical School Xi''an Jiaotong University Xi''an 710081 China National Research Institute for Family Planning Beijin 100081 China National Research Institute for Family Planning Beijin 100081 China

Objective In order to gain further information about the mechanism of emergency contraception, during the peri-implantation period the effect of single and low dose of Mifepristone on the expression of integrin level in endometrium of healthy women were observed Materials ＆ Methods Eleven healthy women, proven fertility, were randomly divided into two groups: group LH-2 (n = 5) and LH+ 2 (n = 6). During the control and treatment cycle, placebo or 10 mg Mifepristone were given orally to group LH- 2 on cycle day LH- 2 or to group LH + 2 on cycle day LH - 2, respectively. One endometrial tissue specimen was obtained in both the control and the treatment cycle on cycle day LH+ *** this study, the endometrial specimens were assessed by immunohistochemistry analysis to measure the change of integrin α1, α4 and β3 subunits expression on the endometrium after using *** Mifepristone increased the expression of α1 and α4 subunit in glandular epithelium only in group LH- 2. The expression of β3 integrin subunit remain the same in our *** Treatment with Mifepristone interferes with integrin distribution during the implantation period, which may imply that the contraceptive effect of Mi fepristone is primarily due to impaired endometrial receptivity.

关键词： Mifepristone, integrin, endometrium, peri-implantation period

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Reorganization of cytoskeletal proteins of mouse oocytes mediated by integrins

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Science China(Life Sciences) 2004年第6期47卷 540-544页

作者： YUE Limin1, ZHANG Lei1, HE Yaping1, ZHANG Jinhu1, ZHENG Jie1, HE Yanfang1, ZHENG Yu1, ZHANG Jie2 & ZHANG Li2 1. Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China 2. West China Hospital of Sichuan University, Chengdu 610041, China Correspondence should be addressed to Yue Limin 1. Department of Physiology West China School of Preclinical and Forensic Medicine Sichuan University 610041 Chengdu China 2. West China Hospital of Sichuan University 610041 Chengdu China

To study whether integrins on cell membrane ligate with intracellular cytoskeletal proteins and mediate their reorganization in egg activation, female mice were used for su- perovulation. The zona-free oocytes were incubated separately with specific ligand of integrins, an active RGD peptide, in vitro for certain period of time. RGE peptide and mouse capacitated sperm were used as controls. Freshly ovulated oocytes and those treated with different factors were immunostained with FITC-labeled anti-actin antibody, then detected with confocal micro- scope. The results demonstrated that freshly ovulated mouse oocytes, oocytes incubated for 2 h in vitro and those treated with control RGE peptide for 15 min showed hardly visible fluorescene or only thin fluorescence in plasma membrane region. Oocytes coincubated with sperms for 15 min and those treated with active RGD peptide for 10 min, 30 min and 2 hours respectively had strong and thick fluorescence in the plasma membrane and cortical region of oocytes, and some of them showed asymmetrically fluorescent distribution. It is proved that integrins on membrane are ligated directly with cytoskeletal protein. integrins binding with their ligands regulate reor- ganization of cytoskelal protein, which may be involved in transmembrane signaling in egg acti- vation.

关键词： integrin, mouse oocyte, cytoskeletal protein.

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