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Different Optical Properties between Human Hepatocellular Carcinoma Tissues and Non-tumorous Hepatic Tissues In Vitro

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2011年第4期31卷 515-519页

作者：于愿肖朝文陈堃郑建伟张俊赵新阳薛新波 Department of Biliary and Pancreatic Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology

There has been an ongoing search for clinically acceptable methods for the accurate,efficient and simple diagnosis and prognosis of hepatocellular carcinoma（HCC）.Optical spectroscopy is a technique with potential clinical applications to diagnose cancer diseases.The purpose of this study was to obtain the optical properties of HCC tissues and non-tumorous hepatic tissues and identify the difference between them.A total of 55 tissue samples（HCC tissue,n=38;non-tumorous hepatic tissue,n=17） were surgically resected from patients with HCC.The optical parameters were measured in 10-nm steps using single-integrating-sphere system in the wavelength range of 400 to 1800 nm.It was found that the optical properties and their differences varied with the wavelength for the HCC tissue and the non-tumorous hepatic tissue in the entire wavelength range of research.The absorption coefficient of the HCC tissue（1.48±0.99,1.46±0.88,0.86±0.61,2.15±0.53,0.54±0.10,0.79±0.15 mm-1） was significantly lower than that of the non-tumorous hepatic tissue（2.79±1.73,3.13±1.47,3.06±2.79,2.57±0.55,0.62±0.10,0.93±0.16 mm-1） at wavelengths of 400,410,450,1450,1660 and 1800 nm,respectively（P0.05）.The reduced scattering coefficient of HCC tissue（5.28±1.70,4.91±1.54,1.26±0.35 mm-1） and non-tumorous hepatic tissue（8.14±3.70,9.27±3.08,2.55±0.57 mm-1） was significantly different at 460,500 and 1800 nm respectively（P0.05）.These results show different pathologic liver tissues have different optical properties.It provides a better understanding of the relationship between optical parameters and physiological characteristics in human liver tissues.And it would be very useful for developing a non-invasive,real-time,simple and efficient way for medical management of HCC in the future.

关键词： carcinoma hepatocellular optical properties integrating sphere

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Effect of purification,dehydration,and coagulation processes on the optical parameters of biological tissues

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Chinese Optics Letters 2021年第1期19卷 69-74页

作者： Halil Arslan Bahar Pehlivanoz Faculty of Technology Electrical and Electronics Engineering DepartmentSakarya University of Applied SciencesSakaryaTurkey

In this study,the effects of purification,dehydration,and coagulation processes on the absorption and reduced scattering coefficients of chicken liver tissues have been investigated by using a single integrating sphere system.The purification process performed on the tissue samples to remove blood residue has been found to cause a slight change in the optical parameters.Although the dehydration process brings about an increase in the absorption coefficient due to the water loss,no direct relationship has been observed between the reduced scattering coefficient and the dehydration level of the tissue.In addition,it has been observed that there was a relatively small increase in the absorption coefficient and a significant increase in the reduced scattering coefficient after the coagulation process.Therefore,it can be said that the optical penetration depth decreased significantly after dehydration and coagulation processes unlike blood purification.Moreover,fluence rate distributions inside the fresh,blood purified,dehydrated,and coagulated tissue models have been investigated by using the Monte Carlo modeling of photon transport in multilayered tissues simulation code.

关键词： optical properties dehydration coagulation integrating sphere IAD

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A SIMPLE METHOD FOR PREDICTION OF THE REDUCED SCATTERING COEFFICIENT IN TISSUE-SIMULATING PHANTOMS

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Journal of Innovative Optical Health Sciences 2010年第1期3卷 53-59页

作者： JIANWEI FU GUOTAO QUAN HUI GONG Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics—Huazhong University of Science and Technology Wuhan 430074P.R.China

This paper proposes a method for predicting the reduced scattering coefficients of tissuesimulating phantoms or the desired amount of scatters for producing phantoms according to Mie scattering theory without measurements with other instruments.The concentration of the scatters TiO2 particles is determined according to Mie theory calculation and added to transparent host epoxy resin to produce phantoms with different reduced scattering coefficients.Black India Ink is added to alter the absorption coefficients of the phantoms.The reduced scattering coefficients of phantoms are measured with single integrating sphere system.The results show that the measurements are in direct proportion to the concentration of TiO2 and have identical with Mie theory calculation at multiple wavelengths.The method proposed can accurately determine the concentration of scatters in the phantoms to ensure the phantoms are qualified with desired reduced scattering coefficients at specified wavelength.This investigation should be possible to manufacture the phantom simply in reasonably accurate for evaluation of biomedical optical imaging systems.

关键词： Mie scattering theory integrating sphere optical imaging

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REALIZATION OF TOTAL SPECTRAL RADIANT FLUX SCALE AND CALIBRATION SERVICE AT NIST

REALIZATION OF TOTAL SPECTRAL RADIANT FLUX SCALE AND CALIBRA...

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26th Session of the CIE

作者： Yoshi Ohno National Institute of Standards and Technology Gaithersburg MD 20899USA

＜正＞In response to the increasing needs for standards to calibrate sphere-spectroradiometer systems used to measure various types of light sources,we have realized the total spectral radiant flux （TSRF）scale,developed transfer standard lamps,and established a calibration service for incandescent standard lamps in the spectral range of 360 nm to 830 nm with relative expanded uncertainties（k=2）between 1%（at visible and near infrared regions）to 2 %（in ultraviolet（UV） region）.The TSRF scale is based on both the NIST spectral irradiance scale and the total luminous flux scale and is realized by using a gonio-spectroradiometer for measuring relative TSRF and a detector-based absolute integrating sphere for measuring total luminous flux.

关键词： calibration spectral radiant flux total flux integrating sphere spectroradiometer measurement standard lamp scale

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