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insulin-like growth factor-i receptor in proliferation and motility of pancreatic cancer

World Journal of Gastroenterology 2010年第15期16卷 1854-1858页

作者： Minoru Tomizawa Fuminobu Shinozaki Takao Sugiyama Shigenori Yamamoto Makoto Sueishi Takanobu Yoshida Department of Gastroenterology National Hospital Organization Shimoshizu Hospital Department of Radiology National Hospital Organization Shimoshizu Hospital Department of Rheu-matology National Hospital Organization Shimoshizu Hospital Department of Pediatrics National Hospital Organization Shimoshizu Hospital Department of internal Medicine National Hospital Organization Shimoshizu Hospital

AiM:To develop a molecular therapy for pancreatic cancer, the insulin-like growth factor-i (iGF-i) signaling pathway was ***: Pancreatic cancer cell lines (MiA-Paca2, NOR-P1, PANC-1, PK-45H, PK-1, PK-59 and KP-4) were cultured in media with 10 mL/L fetal bovine serum. Western blotting analysis was performed to clarify the expression of iGF-i receptor (iGF-iR). Picropodophyllin (PPP), a specific inhibitor of iGF-iR, LY294002, a specific inhibitor of phosphatidylinositol3 kinase (Pi3K), and PD98059, a specific inhibitor of mitogen-activated protein kinase, were added to the media. After 72 h, a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay was performed to analyze cell proliferation. A wound assay was performed to analyze cell motility with hematoxylin and eosin (HE) staining 48 h after addition of each inhibitor. RESULTS: All cell lines clearly expressed not only iGF-iR but also phosphorylated iGF-iR. PPP significantly suppressed proliferation of MiA-Paca2, NOR-P1, PANC-1, PK-45H, PK-1, PK-59 and KP-4 cells to 36.9% ± 2.4% (mean ± SD), 30.9% ± 5.5%, 23.8% ± 3.9%, 37.1% ± 5.3%, 10.4% ± 4.5%, 52.5% ± 4.5% and 22.6% ± 0.4%, at 2 μmol/L, respectively (P ignificantly suppressed proliferation of MiA-Paca2, NOR-P1, PANC-1, PK-45H, PK-1, PK-59 and KP-4 cells to 44.4% ± 7.6%, 32.9% ± 8.2%, 53.9% ± 8.0%, 52.8% ± 4.0%, 32.3% ± 4.2%, 51.8% ± 4.5%, and 30.6% ± 9.4%, at 50 μmol/L, respectively (P id not significantly suppress cell proliferation. PPP at 2 μmol/L suppressed motility of MiA-Paca2, NOR-P1, PANC-1, PK-45H, PK-1, PK-59 and KP-4 cells to 3.0% ± 0.2%, 0%, 0%, 2.0% ± 0.1%, 5.0% ± 0.2%, 3.0% ± 0.1%, and 5.0% ± 0.2%, respectively (P ility of MiA-Paca2, NOR-P1, PANC-1, PK-45H, PK-1, PK-59 and KP-4 to 3.0% ± 0.2%, 0%, 3.0% ± 0.2%, 0%, 0%, 0% and 3% ± 0.1%, respectively (P id not suppress

关键词： insulin-like growth factor-i receptor Phosphatidylinositol 3 kinase Pancreatic neoplasms

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inhitory effect of ginsenoside RG1 on inflammation-induced dopaminergic neuron-degeneration by iGF-iR and GR

Inhitory effect of ginsenoside RG1 on inflammation-induced d...

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中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：孙宪昌陈文芳青岛大学医学院生理教研室

Ginsenoside Rg1 is the main pharmacologically active compound of *** studies have shown that Rg1 possess a variety of beneficial effects on human health,including anti-inflammatory,antioxidant,***,the signaling pathways underlying the anti-inflammatory effect in the central nervous system remain *** the present study,The LPS-induced rat‘model’of PD was used to test the protective effect of *** injection of LPS induced activation of microglial cells,loss of TH immunostaining and decreased content of DA and its metabolites in the lesioned side of ***1protected mesencephalic dopaminergic neuron from degeneration induced by *** effect was partly blocked by iGF-iR antagonist JB-1 and glucocorticoid receptor antagonist *** of Rg1could reverse the LPS-induced the production of NO、TNF-α及iL-1βand this effect could also be partly blocked by JB-1 and ***-treatment with the JB-1 or GR partly blocked the effect of Rg1 on the LPS-induced increase of p-ERK、p-JNK、p-38 and p-iκB protein expressions in the substantial *** results indicated that Rg1 has neuroprotective effects on mesencephalic dopaminergic neurons by inhibiting the production of proinflammatory *** mechanism may be related to iGF-iR and GR signaling pathways.

关键词： ginsenoside Rg1 lipopolysaccharide insulin-like growth factor-i receptor glucocorticoid receptor Parkinson’s disease

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