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The expression of insulin-like growth factor-ⅰ mRNA and polypeptide in rat osteoblasts with exposure to parathyroid hormone

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Chinese Medical Journal 2003年第12期116卷 1916-1922页

作者：张克勤陈家伟王美莲汪承亚李光富郑肇熙赵人铮 Departments of Endocrinology The First Affiliated Hospital of Nanjing Medical University Departments of Endocrinology Obstetrics & Gynecology The First Affiliated Hospital of Nanjing Medical University Institute of Molecular Biology and Institute of Applied Toxicology Nanjing Medical University Departments of Endocrinology and Pathology The First Affiliated Hospital of Nanjing Medical University Institute of Molecular Biology Nanjing Medical University

Objective To investigate the insulin-like growth factor-ⅰ (IGF-Ⅰ) gene and polypeptide expression in cultured rat osteoblast (ROB) and the role of IGF-Ⅰ in mediating the cell-to-cell communication by mimicking the pharmacokinetics of parathyroid hormone (PTH). Methods The ROB was cultured with three kinds of treatment: (1) Control (Ctr), the cells were cultured without PTH during the first 6 hours and the subsequent 42 hours in a 48-hour cycle; (2) Intermittent exposure to PTH (Itm), the cells were cultured with PTH during the first 6 hours, but without PTH in the subsequent 42 hours; and (3) Continuous exposure to PTH (Ctu), the cells were cultured with PTH during the first 6 hours and the subsequent 42 hours. Results The bone-forming activities of ROB were increased in Itm and inhibited in Ctu. The IGF-Ⅰ mRNA content in Itm cells was elevated only during the first 6 hours and that in Ctu cells was elevated at any time during an incubation cycle. The free IGF-Ⅰ concentration in the medium of Itm cells was generally higher and that of the Ctu cells was generally lower compared with those of the Ctr cells. The IGF-Ⅰ antibody significantly reduced the alkaline phosphatase activity within the cells of Ctr and Itm. Conclusions PTH rapidly and constantly stimulates the IGF-Ⅰ gene transcription of osteoblast. There was an obvious discrepancy between the IGF-Ⅰ mRNA content within the osteoblast and the free IGF-Ⅰ level around the osteoblast in either mode of PTH action. The IGF-Ⅰ might be important for osteoblast-osteoblast communication and bone-forming activity, not only in intermittent PTH administration, but also in the physiological functioning of osteoblasts.

关键词： parathyroid hormone osteoporosis osteoblast cytology insulin-like growth factor-ⅰ

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Effects of ethanol on insulin-like growth factor-ⅰ system in primary cultured rat hepatocytes: Implications of JNK1/2 and alcoholdehydrogenase

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World Journal of Gastroenterology 2008年第27期14卷 4324-4331页

作者： Young-Il Oh Jong-Hoon Kim Chang-Won Kang Department of Veterinary Physiology College of Veterinary Medicine Bio-Safety Research Institute Chonbuk National University

AIM: To evaluate the effects of ethanol on the insulin- like growth factor-Ⅰ (IGF-Ⅰ) system involved in c-Jun N-terminal kinase (JNK1/2) and alcoholdehydrogenase (ADH) activity in primary cultured rat hepatocytes. METHODS: Hepatocytes isolated from male Sprague-Dawley rats were incubated with various concentrations of ethanol for different durations of time. The cells were pretreated with SP600125 (10 μmol/L) and 4-MP (200 μmol/L), and then treated with ethanol (200 mmol/L). We then measured IGF-Ⅰ secretion, IGF-Ⅰ mRNA expression, cell viability and JNK1/2 activity by radioimmunoassay, RT-PCR, MTT assay and Western blot, respectively (n = 6). RESULTS: Ethanol induced the activity of phospho (p)-JNK1/2, reaching a maximum at 60 min and then decreasing at 180 min. The effects of ethanol on the IGF-Ⅰ system were increased at 60 min (secretion: 7.11 ± 0.59 ng/mg protein vs 4.91 ± 0.51 ng/mg, mRNA expression: 150.2% ± 10.2% vs 101.5% ± 11.3%, P = 0.045) and then decreased at 180 min (secretion: 3.89 ± 0.25 ng/mg vs 5.4 ± 0.54 ng/mg protein; mRNA expression: 41.5% ± 10.4% vs 84.7% ± 12.1%, P = 0.04), however cell viability was decreased in a dose- and time-dependent manner. SP600125 blocked the ethanol-induced changes (at 60 min). Additionally, 4-methylpyrazole prevented the ethanol-induced decreases in the IGF-Ⅰ system, cell viability and p-JNK1/2 activity (at 180 min). CONCLUSION: This study suggests that ethanol- induced p-JNK1/2 activation is associated with the IGF-Ⅰ system and cell viability in hepatocytes. Furthermore, alcohol dehydrogenase is involved in the relationship between ethanol-induced inactivation of p-JNK1/2 and the changes of the IGF-Ⅰ system and cell viability.

关键词： insulin-like growth factor-ⅰ insulin-like growth factor-ⅰ receptor C-Jun N-terminal kinase Hepatocyte Ethanol

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Effects of ginsenoside Rg1 and insulin-like growth factor-ⅰon 6-OHDA-induced neurotoxicity in SK-N-SH cells

Effects of ginsenoside Rg1 and insulin-like growth factor-Ⅰ...

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2012国际生理学学术大会

作者： Yan Z. Wu L. Xue D.D. Chen W.F. Department of Physiology Medical College of Qingdao University

＜正＞Both ginsenoside Rg1 and insulin-like growth factor-I（IGF-I） has been reported to exert neurotropic and neuroprotective effects on the nervous *** present study was designed to investigate the neuroprotective effects of Rgl,IGF-I or Rg1 combined with IGF-I on 6-OHDA-induced neurotoxicity in SK-N-SH *** with Rgl or IGF-I significantly increased the cell viability against 6-OHDA-induced *** Rgl or IGF-I could attenuate 6-OHDA-induced decrease in mitochondrial membrane potential.6-OHDA-induced up-regulation of Bax protein expression could be restored by Rgl or IGF-I treatment. Cotreatment with Rgl and IGF-I resulted in an enhancement of cell *** results suggest that ginsenoside Rgl and IGF-I have neuroprotective effects against 6-OHDA-induced apoptosis in SK-N-SH *** with Rgl and IGF-I have synergistic effect on cell survival in SK-N-SH cells.

关键词： ginsenoside Rg1 6-OHDA SK-N-SH cells insulin-like growth factor-ⅰ

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Branched-chain amino acids partially recover the reduced growth of pigs fed with protein-restricted diets through both central and peripheral factors

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Animal Nutrition 2021年第3期7卷 868-882页

作者： Mohammad Habibi Cedrick Shili Julia Sutton Parniyan Goodarzi Excel Rio Maylem Leon Spicer Adel Pezeshki Department of Animal and Food Sciences Oklahoma State UniversityStillwaterOK74078USA

The objective of this study was to assess the growth efficiency of pigs fed with protein-restricted diets supplemented with branched-chain amino acids(BCAA)and limiting amino acids(LAA)above the rec-ommended *** 2 weeks of adaptation,48 young barrows were weight matched and randomly assigned to 6 treatments(8 pigs/treatment)for 4 weeks:positive control(PC)with standard protein,negative control(NC)with very low protein containing LAA(i.e.,Lys,Met,Thr and Trp)at rec-ommended levels,and NC containing LAA 25%(L25),LAA 50%(L50),LAA+BCAA(i.e.,Leu,Ile and Val)25%(LB25)and LAA+BCAA 50%(LB50)more than *** intake(FI)and body weight(BW)were measured daily and weekly,*** week 6,blood samples were collected,all pigs euthanized and tissue samples *** data were analyzed by univariate GLM or mixed procedure(SPSS)and the means were separated using paired Student's t-test followed by Benjamini-Hochberg *** to PC,NC had decreased FI,BW,unsupplemented plasma essential amino acids,serum insulin-like growth factor-I(IGF-I)and hypothalamic neuropeptide Y(NPY)(Pgrowth through improved IGF-I concentration and FI,which was associated with changed expression of serotonin receptors,blood AA and hypothalamic NPY.

关键词： Branched-chain amino acid Very low protein diet NPY insulin-like growth factor-ⅰ Serotonin Pig

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