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Role of DSC1 in Drosophila melanogaster synaptic activities in response to haedoxan A

insect Science 2023年第6期30卷 1677-1688页

作者： Xingtao Qie Yaxin Ren Xueting Chen Yuzhe Du Ke Dong Zhaonong Hu Institution of Pesticide Science College of Plant ProtectionNorthwest A&F UniversityYanglingShaanxi ProvinceChina Key Laboratory of Botanical Pesticide R&D in Shaanxi Province YanglingShaanxi ProvinceChina Department of Plant Protection College of Plant ProtectionShanxi Agricultural UniversityTaiguShanxi ProvinceChina Southern insect Management Research Unit Agricultural Research ServiceUnited States Department of AgricultureStonevilleMSUSA Department of Biology Duke UniversityDurhamNCUSA Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau Ministry of AgricultureNorthwest A&F UniversityYanglingShanxi ProvinceChina

Drosophila sodium channel 1 (DSC1) encodes a voltage-gated divalent cation channel that mediates neuronal excitability in insects. Previous research revealed that DSC1 knockout Drosophila melanogaster conferred different susceptibility to insecticides, which indicated the vital regulation role of DSC1 under insecticide stress. Haedoxan A (HA) is a lignan compound isolated from Phryma leptostachya, and we found that HA has excellent insecticidal activity and is worthy of further study as a botanical insecticide. Herein, we performed bioassay and electrophysiological experiments to test the biological and neural changes in the larval Drosophila with/without DSC1 knockout in response to HA. Bioassay results showed that knockout of DSC1 reduced the sensitivity to HA in both w1118 (a common wild-type strain in the laboratory) and parats1 (a pyrethroid-resistant strain) larvae. Except for parats1/DSC1−/−, electrophysiology results implicated that HA delayed the decay rate and increased the frequency of miniature excitatory junctional potentials of Drosophila from w1118, parats1, and DSC1−/− strains. Moreover, the neuromuscular synapse excitatory activities of parats1/DSC1−/− larvae were more sensitive to HA than DSC1−/− larvae, which further confirmed the functional contribution of DSC1 to neuronal excitability. Collectively, these results indicated that the DSC1 channel not only regulated the insecticidal activity of HA, but also maintained the stability of neural circuits through functional interaction with voltage-gated sodium channels. Therefore, our study provides useful information for elucidating the regulatory mechanism of DSC1 in the neural system of insects involving the action of HA derived from P. leptostachya.

关键词： botanical insecticide Drosophila sodium channel 1(DSCI) insect nervous system neuromuscular synapse activity regulatory

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