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Journal of Animal Science and Biotechnology 2024年第2期15卷 699-712页

作者： Liz Vanessa Lagos Mike Richard Bedford Hans Henrik Stein Division of Nutritional Sciences University of IllinoisUrbanaIL 61801USA AB Vista Marlborough SN84ANUK

Background The effect of microbial phytase on amino acid and energy digestibility is not consistent in pigs,which may be related to the phytase dosage or the adaptation length to the diet.Therefore,an experiment was conducted to test the hypotheses that increasing dietary phytase after an 18-day adaptation period:1)increases nutrient and energy digestibility;2)increases plasma P,plasma inositol,and bone ash of young pigs;and 3)demonstrates that maximum phytate degradation requires more phytase than maximum P digestibility.Results Data indicated that increasing inclusion of phytase[0,250,500,1,000,2,000,and 4,000 phytase units(FTU)/kg feed]in corn-soybean meal-based diets increased apparent ileal digestibility(AID)of Trp(quadratic;Pinositol also increased(linear;Pinositol phosphate(IP)6 and IP5(quadratic;Pinositol concentrations(linear;Pinositol release.Conclusions Increasing dietary levels of phytase after an 18-day adaptation period increased phytate and IP ester degradation and inositol release in the small intestine.Consequently,increasing dietary phytase resulted in improved digestibility of Ca,P,K,Na,and the first 4 limiting amino acids,and in increased concentrations of bone ash and plasma P and inositol.In a corn-soybean meal diet,maximum inositol release requires approximately 3,200 FTU/kg more phytase than that re

关键词： Bone ash inositol Nutrient digestibility Phytase Phytate degradation Pigs

Myo-inositol versus metformin effects on clinical features, endocrine and metabolic profiles in infertile women with polycystic ovary syndrome: A randomized controlled trial

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Asian pacific Journal of Reproduction 2023年第6期12卷 256-265页

作者： Nguyen Sa Viet Le Minh Tam Le Thanh Ngoc Cao Center for OBGYN Hue Central Hospital16 Le Loi StreetHueVietnam Center for Reproductive Endocrinology and Infertility Hue University of Medicine and PharmacyHue University06 Ngo Quyen StreetHueVietnam

Objective:To compare the effectiveness of inositol and metformin on the clinical characteristics,and endocrine and metabolic profiles of infertile polycystic ovarian syndrome(PCOS)women from Vietnam.Methods:From June 2018 to August 2022,a randomized trial was undertaken at the Hue Center for Endocrinology and Reproduction on infertile women aged 18 to 40 years with polycystic ovarian syndrome.The clinical,endocrine,and metabolic features of these individuals were assessed before and after 3 months of treatment with 2 g of inositol or 1700 mg of metformin per day.Natural pregnancy rates,adverse effects,and tolerance of inositol were recorded.Results:The study included 171 infertile PCOS women who were eligible to participate and took part in the baseline assessment,of whom 132 women participated in data analysis after 3 months.After metformin treatment,42.1%of women with oligomenorrhea experienced regular menstruation.Metformin significantly lowered body mass index(BMI),waist circumference and testosterone levels,but had no effect on other clinical characteristics,endocrine profiles,or metabolic profiles.29.2%Of women reported experiencing side effects.21%Of them attained pregnancy,which resulted in 17.1%of live births.In the inositol group,the rate of regular cycle increased by 18.2%and the total testosterone concentration significantly decreased.In overweight/obese women with PCOS,inositol significantly decreased weight,BMI,waist and hip circumferences(Pinositol and continued treatment.18.9%Of them became pregnant,leading to 17%of live births.Conclusions:Metformin and inositol can improve weight and waist circumference in overweight/obese infertile women with PCOS.Metformin is associated with a higher rate of regular menstruation,whereas inositol is associated with a lower rate of adverse effects.The spontaneous conception,clinical pregnancy,and live birth rates between two groups are comparable.

关键词： Polycystic ovarian syndrome inositol Metformin Endocrine Metabolic Menstrual cycle Pregnancy

Sampling duration and freezing temperature influence the analysed gastric inositol phosphate composition of pigs fed diets with different levels of phytase

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Animal Nutrition 2019年第2期5卷 196-201页

作者： Steven Laird Imke Kühn Michael R.Bedford Hayley Whitfield Helen M.Miller Faculty of Biological Sciences University of Leeds AB Vista School of Biological Sciences University of East Anglia

This experiment was conducted to determine the effects of time and freezing temperature during sampling on gastric phytate(myo-inositol [MYO] hexakisphosphate [InsP6]), lower inositol phosphates(InsP2-5) and MYO concentrations in pigs fed diets containing different levels of phytase. Forty pigs were fed 1 of 4 wheat-barley diets on an ad libitum basis for 28 d. The diets comprised a nutritionally adequate positive control(PC), a similar diet but with Ca and P reduced by 1.6 and 1.24 g/kg, respectively(NC), and the NC supplemented with 500(NC + 500) or 2,000(NC + 2000) FTU phytase/kg. At the end of the experiment, chyme were collected from the stomach, thoroughly mixed and 2 subsamples(30 mL) were frozen immediately: one snap-frozen at-79 ℃ and the other at-20 0 C. The remaining chyme were left to sit at room temperature(20 ℃) and further subsamples were collected and frozen as above at 5,10 and15 min from the point of mixing. There were linear reductions in gastric InsP6 concentration over time during sampling(P inositol phosphate profile, chyme should be snap-frozen immediately after collection.

关键词： Pig inositol phosphate Phytase Sampling time Freezing temperature

Participation of the inositol 1,4,5-trisphosphategated calcium channel in the zona pellucida- and progesterone-induced acrosome reaction and calcium influx in human spermatozoa

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Asian Journal of Andrology 2020年第2期22卷 192-199页

作者： Ying-Ya Li Yan-Ping Jia Li-Yan Duan Kun-Ming Li Center of Reproductive Medicine Shanghai First Maternity and Infant HospitalTongji University School of MedicineShanghai 201204China

The acrosome reaction is a prerequisite for fertilization,and its signaling pathway has been investigated for decades.Regardless of the type of inducers present,the acrosome reaction is ultimately mediated by the elevation of cytosolic calcium.inositol 1,4,5-trisphosphate-gated calcium channels are important components of the acrosome reaction signaling pathway and have been confirmed by several researchers.In this study,we used a novel permeabilization tool BioPORTER?and first demonstrated its effectiveness in spermatozoa.The inositol 1,4,5-trisphosphate type-1 receptor antibody was introduced into spermatozoa by BioPORTER?and significantly reduced the calcium influx and acrosome reaction induced by progesterone,solubilized zona pellucida,and the calcium ionophore A23187.This finding indicates that the inositol 1,4,5-trisphosphate type-1 receptor antibody is a valid inositol 1,4,5-trisphosphate receptor inhibitor and provides evidence of inositol 1,4,5-trisphosphate-gated calcium channel involvement in the acrosome reaction in human spermatozoa.Moreover,we demonstrated that the transfer of 1,4,5-trisphosphate into spermatozoa induced acrosome reactions,which provides more reliable evidence for this process.In addition,by treating the spermatozoa with inositol 1,4,5-trisphosphate/BioPORTER?in the presence or absence of calcium in the culture medium,we showed that the opening of inositol 1,4,5-trisphosphate-gated calcium channels led to extracellular calcium influx.This particular extracellular calcium influx may be the major process of the final step of the acrosome reaction signaling pathway.

关键词： acrosome reaction human spermatozoa inositol 1,4,5-trisphosphate zona pellucida progesterone

High expression of type I inositol 1,4,5-trisphosphate receptor in the kidney of rats with hepatorenal syndrome

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World Journal of Gastroenterology 2018年第29期24卷 3273-3280页

作者： Jing-Bo Wang Ye Gu Ming-Xiang Zhang Shun Yang Yan Wang Wei Wang Xi-Ran Li Yi-Tong Zhao Hai-Tao Wang Liver Cirrhosis Ward the Sixth People’s Hospital of ShenyangShenyang 110006Liaoning ProvinceChina Liaoning Cancer Hospital&Institute Shenyang 110042Liaoning ProvinceChina Department of General Surgery the Second Affiliated Hospital of Shenyang Medical CollegeShenyang 110002Liaoning ProvinceChina

AIM To detect the expression of typeⅠ inositol 1,4,5-trisphosphate receptor(IP3 RI) in the kidney of rats with hepatorenal syndrome(HRS).METHODS One hundred and twenty-five Sprague-Dawley rats were randomly divided into four groups to receive an intravenous injection of D-galactosamine(D-Gal N) plus lipopolysaccharide(LPS; group G/L, n = 50), D-Gal N alone(group G, n = 25), LPS alone(group L, n = 25), and normal saline(group NS, n = 25), respectively.At 3, 6, 9, 12, and 24 h after injection, blood, liver, and kidney samples were collected. Hematoxylineosin staining of liver tissue was performed to assess hepatocyte necrosis. Electron microscopy was used to observe ultrastructural changes in the kidney. Western blot analysis and real-time PCR were performed to detect the expression of IP3 RI protein and m RNA in the kidney, respectively.RESULTS Hepatocyte necrosis was aggravated gradually, which was most significant at 12 h after treatment with D-galactosamine/lipopolysaccharide, and was characterized by massive hepatocyte necrosis. At the same time, serum levels of biochemical indicators including liver and kidney function indexes were all significantly changed. The structure of the renal glomerulus and tubules was normal at all time points. Western blot analysis indicated that IP3 RI protein expression began to rise at 3 h(P < 0.05) and peaked at 12 h(P < 0.01). Real-time PCR demonstrated that IP3 RI m RNA expression began to rise at 3 h(P < 0.05) and peaked at 9 h(P < 0.01).CONCLUSION IP3 RI protein expression is increased in the kidney of HRS rats, and may be regulated at the transcriptional level.

关键词： Hepatorenal syndrome type I inositol 1,4,5-trisphosphate receptor Glomerular mesangial cells Vascular smooth muscle cells

Possible role of inositol phospholipid signal transduction system in Dunaliella salina under hypoosmotic shock

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Chinese Science Bulletin 1997年第2期42卷 151-155页

作者： Chen, SX Ll, L Yan, JQ Xu, ZK Jiao, XZ Shanghai Institute of Plant Physiology Chinese Academy of Sciences Shanghai China Department of Biology East China Normal University Shanghai China

IN the course of plant life, many physiological and biochemical processes are controlled by extracellular signals (light, temperature, gravity, water, hormone, etc. ). How the cells perceive these environmental signals, transduce them inwards and lead to alterations of intracellular physiology has become a focus in life science these years. Research in this field was started earher and further developed in animal cells. When specific receptors on the external surfaceof ammal cells perceive an extracellular signal, specific phospholipase C (PLC) isozymes are activated, signaled by the cleavage of inositol phospholipids to release second messengers, i.e.

关键词： Dunaliella salina inositol phospholipid signal transduction system hypoosmotic shock.

Temperature-dependence and conformational basis of inositol 1,4,5-trisphosphate receptor regulated by Ca^(2+)

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Science China(Life Sciences) 2000年第3期43卷 225-231页

作者：胡广黄有国杨福愉 National Laboratory Blomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101China

The inositol 1,4,5-trisphosphate (lnsP3) receptor was purified from bovine cerebellum and reconstituted in liposomes composed of phosphatidylcholine (PC) and phosphatidylethanola-mine (PE) (1:1) successfully. No effect of Ca2+ concentration on [3H]-lnsP3 binding to unreconsti-tuted lnsP3 receptor could be observed either at 4℃ or at 25℃, whereas the effect of [Ca2+] on reconstituted lnsP3 receptor depended on the temperature. The Ca2+ concentration outside the proteolipsome ([Ca2+]o) had no detectable effect on lnsP3 binding to lnsP3 receptor at 4℃. In contrast, with increase of [Ca2+]o from 0 to 100 nmol/L at 25℃, the lnsP3 binding activity increased gradually. Then the lnsP3 binding activity was decreased drastically at higher [Ca2+]0 and inhibited entirely at 50 nmol/L [Ca2+]. Conformational studies on intrinsic fluorescence of the reconstituted lnsP3 receptor and its quenching by Kl and HB indicated that the global conformation of reconstituted lnsP3 receptor could not be affected by [Ca2+]o

关键词： Ca2+ inositol 1 4 5-trisphosphate receptor reconstitution temperature conformation.

Price of inositol Drops Dramatically

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China Chemical Reporter 2009年第6期20卷 18-18页

作者： Zhang Yemei,Lycored Representative in China Lycored Representative in China

China’s Vitamin B12 price has a sharp dive after the Beijing Olympics Games,the same story also happen in inositol.Comparing to peak price in 2008,China’s inositol price has dropped by 60%.

关键词： Vitamin happen dropped Olympics inositol thousand

Quantitative Detection of inositol Hexakisphosphate (InsP6) in Crop Plants Using Polyacrylamide Gel Electrophoresis (PAGE)

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American Journal of Plant Sciences 2013年第12期4卷 1-6页

作者： Mohammad Alimohammadi Nawab Ali Mariya Khodakovskaya Department of Applied Science University of Arkansas at Little Rock Little Rock USA

inositol phosphates are essential for cell development and signaling in all living organisms. inositol hexakisphosphate (InsP6) is the most abundant phosphoinositol in both plants and animals. While the concentration of inorganic phosphorous (Pi) is often limited in soil, some plants overcome this limitation by creating a phosphate reservoir that serves as a source of Pi during phosphate deficiency. Although this strategy benefits plant development and signaling under adverse environmental conditions, excessive accumulation of Pi in crop plants has raised serious concerns about its toxicity and ill effects on human health. Consumption of crop plants with high InsP6 content or food products made from these crops is found to reduce nutrient intake significantly by way of chelating essential metal cations in human and livestock fed by such plants. Therefore, it is necessary to determine InsP6 contents in crop plants. Several methods have been developed for the screening and detection of InsP6 in plants. These detection methods however, are complex, labor-intensive, and often provide inaccurate results. We have developed a fast, reliable, and cost-effective method for the detection and quantification of InsP6 in plants using polyacrylamide gel electrophoresis (PAGE) with potential applications in industry, quality control labs, and research projects.

关键词： inositol Hexakisphosphate Polyacrylamide Gel Electrophoresis High-Performance Liquid Chromatography Nutrient Deficiency

Quantification of inositol Hexa-Kis Phosphate in Environmental Samples

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Open Journal of Soil Science 2012年第1期2卷 55-63页

作者： Lynne P. Heighton Merle Zimmerman Clifford P. Rice Eton E. Codling John A. Tossell Walter F. Schmidt Department of Chemistry and Biochemistry University of Maryland College Park United States EMBUL ARS USDA Beltsville United States

Phosphorous (P) is a major contributor to eutrophication of surface waters, yet a complete understanding of the P cycle remains elusive. inositol hexa-kis phosphate (IHP) is the primary form of organic (PO) in the environment and has been implicated as an important sink in aquatic and terrestrial samples. IHP readily forms complexes in the environment due to the 12 acidic sites on the molecule. Quantification of IHP in environmental samples has typically relied on harsh extraction methods that limit understanding of IHP interactions with potential soil and aquatic complexation partners. The ability to quantify IHP in-situ at the pH of existing soils provides direct access to the role of IHP in the P cycle. Since it is itself a buffer, adjusting the pH correspondingly alters charged species of IHP present in soil. Density Functional Theory (DFT) calculations support the charged species assignments made based pKas associated with the IHP molecule. Raman spectroscopy was used to generate pH dependent spectra of inorganic (PI) and IHP as well as (PO) from IHP and (PI) in soil samples. Electro-spray ionization mass spectroscopy (ESI-MS) was used to quantify IHP-Iron complexes in two soil samples using a neutral aqueous extraction.

关键词： Phytic Acid inositol Hexkis Phosphate Electrospray Mass Spectroscopy (ESI-MS) Density Functional Theory (DFT) Raman Spectroscopy