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中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论...

作者： Jiafei Zhan Hongyu Song Ning Wang Cheng Guo Nengxing Shen Ruiqi Hua Yuan Shi Christiana Angel Xiaobin Gu Yue Xie Weimin Lai Xuerong Peng Guangyou Yang Department of Parasitology College of Veterinary MedicineSichuan Agricultural University Department of Veterinary Parasitology Faculty of Veterinary SciencesShaheed Benazir Bhutto University of Veterinary and Animal Sciences Department of Chemistry College of Life and Basic ScienceSichuan Agricultural University

The larval stage of Echinococcus granulosussensulato,resulting in cystic echinococcosis-a parasitic zoonosis,causes huge economic losses to the livestock industry and poses a threat to public *** of apoptosis proteins(IAPs) is a class of endogenous anti-apoptotic family,which plays a significant functional role in the regulation of organism’s ***,to explore potential functions of IAPs in ***,two members of IAPs from ***(Eg-IAP and Eg-BIRP) were cloned,expressed,and molecularly ***-IAP and Eg-BIRP encoded putative 331 and 168 residue proteins,*** analysis showed that both proteins contained a BIR domain-the essential functional domain of *** immunohistochemistry revealed that both proteins were ubiquitously localized in all tissues of *** except the infertile *** fluorescent quantitative PCR(RT-qPCR) results revealed relatively higher transcription levels of two Eg-IAPs in protoscoleces(PSCs) compared to the adult worms(18 d).We further used different concentrations of LCL161,a Smac-mimetic pan-IAPs inhibitor,to induce the apoptosis in PSCsin vitro,and revealed that the transcription levels of both genes were negatively correlated with the concentration of *** the results of light microscopy,transmission electron microscopy(TEM),and terminal deoxynucleotidyl transferase dUTP nick end labelling(TUNEL) assay also showed a higher apoptotic rate in PSCs with the increasing concentrations of *** together,our findings provide the reasonable evidence that both Eg-IAP and Eg-BIRP have potential implication in critical anti-apoptotic roles during the development of ***.

关键词： cystic echinococcosis Echinococcus granulosussensulato inhibitor of apoptosis proteins anti-apoptotic roles

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Enhanced sensitivity of hepatocellular carcinoma cells to chemotherapy with a Smac-armed oncolytic adenovirus

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Acta Pharmacologica Sinica 2007年第12期28卷 1996-2004页

作者： Qiu-wei PAN~(2,5),Su-yang ZHONG~(2,5),Bi-sheng LIU~2,Jin LIU~2,Rong CAI~2,Yi-gang WANG~2,Xin-yuan LIU~(2,3,4),Cheng QIAN~(2,4)~2 Xin Yuan Institute of Medicine and Biotechnology,Life Science College,Zhejiang Sci-Tech University Hangzhou 310018,China,~3 Instituteof Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China Xin Yuan Institute of Medicine and Biotechnology Life Science College Zhejiang Sci-Tech University Hangzhou China Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Aim:The aim of the present study was to further improve the therapeutic effectsfor human hepatocellular carcinoma (HCC) and reduce the damage in normal cellsusing a novel chemo-gene-virotherapeutic ***:An oncolyticadenoviral vector (ZD55) similar to the typical oncolytic adenovirus ONYX-015,with a deletion of ElB-55K gene,was employed to express the second mitochon-dria-derived activator of caspases (Smac) protein by constructing a *** *** enhanced cytotoxicity of the combined treatment of ZD55-Smac with cisplatin or 5-fluorouracil (5-FU) was evaluated in several HCC ***,the negative effects on normal cells have been tested in humannormal liver cell lines L-02 and QSG-7701 cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and apoptotic cell ***:According to our observation,ZD55-Smac is superior to ONYX-015 in sensitizingchemotherapy,ZD55-Smac used in conjunction with chemotherapy was found toexhibit obviously enhanced cytotoxicity in HCC cells,yet significantly abolishedthe negative toxicity in normal cells by utilizing the tumor selective replicationvector and reducing the ***:This chemo-gene-virotherapeutic(cisplatin or 5-FU+ZD55-Smac) strategy is superior to the conventional chemo-gene or chemo-viro approach.

关键词： oncolytic adenovirus inhibitor of apoptosis proteins second mitochondria-derived activator of caspases chemotherapy hepatocellular carcinoma

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