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Genotypic characterization of Helicobacter pylori isolates among Egyptian patients with upper gastrointestinal diseases

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The Chinese-German Journal of Clinical Oncology 2012年第1期11卷 15-23页

作者： Abdel Hamid Hussein Ezzat Mona Hamza Ali Eman Ahmed El-Seidi Iman Ezzat Wali Nagwa Abd El Rahman Sedky Sherif Medhat Mahmoud Naguib Department of Surgical Oncology National Cancer Institute Cairo University Laboratory Forensic Medicine Egyptian Ministry of Justice Medical Microbiology and Immunology Faculty of Medicine Cairo University Internal Medicine Faculty of Medicine Cairo University

Objective： Over 50% of the world populations are infected with Helicobacter pylori （H. pylori）. Most subjects are asymptomatic; however, in 1994, H. pylori has been categorized as group I carcinogen. The aim of the study was to investigate the relationship between H. pylori infection and gastric cancer. Methods： Thirty gastric cancer patients （GCs） and 30 gastritis patients were enrolled in the study. H. pylori was cultured on non-selective and selective medias, infection density was assessed by quantitative culture. Antibiotic sensitivity testing was performed. PCR was done for the H. pylori 16S rRNA gene in addition to cagA, vacA and iceA genes. Results： H. pylori could be cultured from 100% of specimens obtained from all patients. The density of H. pylori was higher in cancer cases than in gastritis patients. The 16S rRNA was detected in all GC patients （100%） while it was only detected in 70% of gastritis patients. The cagA gene was found in 53.3% vs 13.3% of GC and gastritis patients, respectively. The vacA gene was present in all GC patients （by at least one of its alleles） while it was only found in 33.3% of gastritis patients. The vacA slml combination was the most predominant genotype in GC patients, while m2 was the commonest allele in gastritis patients （10%）. The iceA gene was found in 86.7% vs 40% of GC and gastritis patients, respectively. Simultaneous presence of multiple H. pyiori strains was proved, both phenotypically and genotypically. Conclusion： The development of GC is linked to infection with H. pylori harboring certain virulence genes. Higher infection density of H. pylori was found in GC patients. Co-existence of more than one strain of H. pylori in the same patient occurs in both malignant and benign lesions.

关键词： Helicobacter pylori (H. pylori) pathogenesis virulence genes diagnosis infection density antibiotic resistance PCR

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Prevalence of Wolbachia in 10 Tenebrionidae stored-product insects and spatiotemporal infection dynamics in Tribolium confusum(Jaquelin Du Val)(Coleoptera:Tenebrionidae)

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Grain & Oil Science and Technology 2019年第4期2卷 85-90页

作者： Yujie Lu Shiyuan Miao Zhengyan Wang Sibao Wang College of Food Science and Technology Henan University of TechnologyZhengzhou 450001China Institute of Plant Physiology&Ecology Shanghai Institutes of Biological SciencesChinese Academy of SciencesShanghai 200032China

Insect symbionts Wolbachia used for pest population control has focused on vector pest species and agricultural insects while rare reports in Chinese stored-product insect *** this paper,we surveyed the prevalence of Wolbachia using a PCR detection method in ten Tenebrionidae stored-product ***,the spatiotemporal Wolbachia infection dynamics in Tribolium confusum and Wolbachia elimination patterns using tetracycline treatment were investigated in detail by TaqMan®probe real-time quantitative PCR,and host reproductive fitness parameters were *** was the only Wolbachia infected species in all the surveyed *** infection density consistently increased with the development of *** plateaued at 3.7×107 wsp copies per individual insect at the young adult *** densities in females were higher than that in males with a significant difference at the pupae stage and varied among different tissues and *** female beetles were completely incapable of producing mature progenies when crossed with Wolbachia infected *** and egg hatch rate were specifically inhibited after Wolbachia elimination,while other traits,including the number of eggs,pupation rate and sex ratio,remained unaffected by tetracycline *** results showthat the TaqMan®probe qPCR is a reliable detection method for quantifying the density of Wolbachia as compared to qualitative detection of wsp gene by PCR and relatively quantified by real-time *** fitness results indicated that Wolbachia infection was not an obligate symbiont and benefited the host confused flour beetle.

关键词： Wolbachia Tenebrionidae Tribolium spp. Stored product insects Endosymbiont infection density Tetracycline

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