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Antibody Levels and infection status of Pertussis in the Population under Pertussis Resurgence in Guangxi in 2018:A Cross-Sectional Survey

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Biomedical and Environmental Sciences 2024年第6期37卷 628-638页

作者： Liang Liang Qiuyun Deng Lili Deng Jinghang Wei Shiyi Chen Yizhi Wei Yuyan Ma Yue Qin Wei Liu Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention Nanning 530028GuangxiChina

Objective Pertussis cases have increased markedly since 2018 in *** aim of this study was to evaluate antibody levels and the infection status of pertussis in the resident *** A total of 10,215 serum samples from residents were collected from August-November 2018 and tested for anti-pertussis IgG and toxin IgG using the enzyme-linked immunosorbent assay(ELISA).Results Of the collected samples,1,833(17.94%)tested positive for anti-pertussis IgG,with the median concentration of 16.06 IU/*** levelinfection(≥100 IU/mL).Among the different age groups,the highest rates of positivity and recent infection were observed at 11-20 years of age,the lowest positivity rate at 5 years of age,and the lowest recent infection rate at 4 years of age(P<0.001,P=0.005,respectively).Conclusion The survey results showed that all age groups in Guangxi lacked immunity against pertussis,which was one of the main factors contributing to the resurgence of pertussis in *** addition,the prevalence of pertussis is relatively high in Guangxi,and its incidence is seriously underestimated,especially in adolescents and adults.

关键词： Pertussis resurgence Anti-pertussis IgG Anti-pertussis toxin IgG Antibody level infection status

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Various infection status and molecular evidence for horizontal transmission and recombination of Wolbachia and Cardinium among rice planthoppers and related species

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Insect Science 2013年第3期20卷 329-344页

作者： Kai-Jun Zhang Xiao Han Xiao-Yue Hong Department of Entomology Nanjing Agricultural University Nanjing China

Wolbachia and Cardinium are widely distributed and are considered important for their ability to disturb reproduction and affect other fitness-related traits of their hosts. By using multilocus sequence typing （MLST）, RFLP （restriction fragment length poly- morphism） and 16S ribosomal DNA gene sequencing methods, we extensively surveyed Wolbachia and Cardinium infection status of four predominant rice planthoppers and one kind of leafhopper in different rice fields. The results demonstrated that Sogatellafurcifera （Horv^ith） and Laodelphax striatellus （Fallrn） were infected with the same Wolbachia strain （wStri）, while Nilaparvata lugens （St^l） and its closely related species Nilaparvata muiri China were infected with two phylogeneticlly distant strains, wLug and wMui, respectively. Three new Wolbachia strains （provisionally named wMfasl, wMfas2 and wMfas3） were detected in the leafhopper Macrostelesfascifrons （Stal）. Only S. furcifera was co-infected with Cardinium, which indicated that the distribution of Cardinium in these rice planthop- pers was narrower than that of Wolbachia. Unambiguous intragenic recombination events among these Wolbachia strains and incongruent phylogenetic relationships show that the connections between different Wolbachia strains and hosts were more complex than we expected. These results suggest that horizontal transmission and host associated special- ization are two factors affecting Wolbachia and Cardinium infections among planthoppers and their related species.

关键词： Cardinium infection status intragenic recombination rice planthopper,Wolbachia

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An Evaluation of the infection status and Source of Subgroup J Avian Leukosis Virus in Cloned Free-Range Layers

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Journal of Integrative Agriculture 2013年第4期12卷 687-693页

作者： ZHANG Pei-pei LIU Shao-qiong WANG Jian WANG Bo ZHAO Cheng-di ZHANG Yong-guang SUN Shu-hong College of Veterinary Medicine Shandong Agricultural University Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences

In recent years, subgroup J avian leukosis virus （ALV-J） has been found to frequently infect layers in China. This virus is responsible for economic losses due to both mortality and decreased performance in chickens. In this study, 45-d-old cloned flee-range layers were suspected to be infected with ALV and other immunosuppressive diseases because their feathers were unkempt and their growth rate was impaired. To estimate the infection status and determine the source of ALV-J in the flock, 30 cloacal swabs were randomly collected to measure the p27 antigen level by enzyme-linked immunosorbent assay （ELISA）. Among the birds that were tested, 87% （26/30） were positive. In addition, 6 anticoagulant blood samples were aseptically collected at random from the flock when the layers were 60 d old. These samples were centrifuged to obtain the leukocytes, which were then used to inoculate chicken embryo fibroblast （CEF） cells for the identification of ALV-J by indirect immunofluorescence （IFA）. Of the samples tested, 100% （6/6） were positive. The flock＇s production performance was also investigated, and 10 layers were necropsied to evaluate pathological changes at 115 d of age. The flock never laid eggs even though they reached the age of the first laying （110 d）. Furthermore, there were pathological changes present, including atrophy of the thymus and bursa of Fabricius, undeveloped ovaries, glandular stomach haemorrhage, and hepatosplenomegaly. Paraffin-embedded sections of intumescent liver and spleen were prepared for antigen localisation using IFA. Positive signals were prevalent in paraffin-embedded sections of the intumescent liver and spleen. Furthermore, provirus DNA was extracted from 4 cloned flee-range layers, and 2 patemal parents （HR native cocks）, and the gp85 gene of ALV-J was amplified by PCR to analyse the genetic variation. The results of the autogenous variation analysis showed that the 6 strains were 98.5-99.7% homologous. This study indicated that there

关键词： cloned free-range layers ALV-J infection status hereditary variation source exploration

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