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Transcriptomic Analysis of the Tolerance Response to Dehydration and Rehydration in Wheat Seedlings

Phyton-International Journal of Experimental Botany 2022年第2期91卷 375-394页

作者： Ping Zhang Zhiyou Kong Junna Liu Yongjiang Liu Qianchao Wang Xiuju Huan Li Li Yunfeng Jiang Peng Qin College of Agronomy and Biotechnology Yunnan Agricultural UniversityKunming650201China College of Natural Resources and Environment Baoshan UniversityBaoshan678000China Triticeae Research Institute Sichuan Agricultural UniversityChengdu610000China

Drought is the main abiotic stress that restricts wheat *** rapid development of sequencing technology and its widespread application to various fields have revealed the structural characteristics and regulation of related genes through gene expression ***,we studied responses of wheat plants under drought and rewatering conditions,using morphological and physiological ***,a transcriptome analysis was conducted on Jingmai 12,a drought-resistant wheat strain,to explore the mechanism underlying the response of drought-resistant wheat seedlings to drought stress at the transcriptome *** stress caused morphological and physiological changes in both drought-resistant and-sensitive varieties,but to a greater extent in the drought-sensitive *** re-watering,the drought-resistant wheat showed greater ability to recover than the drought-sensitive *** sequencing of Jingmai 12 revealed 97,422 genes,including 80,373 known genes and 17,049 newly predicted *** observed upregulation of genes was mostly involved in hormone and signal transduction,carbon metabolism,amino acid synthesis,small molecule production,transmembrane transport,ROS detoxification and defense,drought response protein,and protective enzyme *** genes were mostly involved in photosynthesis,lipid metabolism,signaling,and auxin *** rehydration,these genes and metabolic pathways returned to *** results suggest that all these changes are adaptations to drought *** morphological adaptation,physiological regulation,and the expression of drought-induced genes,normal growth of drought-resistant varieties under drought stress can be *** results increase our understanding of the transcriptomic changes taking place in drought-resistant wheat seedlings under drought stress,and provide a direction for future investigations.

关键词： Drought stress inducible gene morphological indicator physiological indicator RNA sequencing wheat seedling

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Identification of a Herbicide Safener AD-67 inducible cDNA in Rice

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Rice science 2007年第3期14卷 235-238页

作者： YIN De Suo SUN Xiao qiong LI Ke WANG Shi quan DENG Qi ming LI Ping Rice Research Institute Sichuan Agricultural UniversityWenjiang 611130China Key Laboratory of Southwest Crop genetic Resource and Improvement Sichuan Agricultural UniversityMinistry of EducationYa＇an625014China Sichuan Agricultural University High-Tech CO． LTDWenjiang 611130China

A herbicide safener AD-67 inducible cDNA was identified in an indica rice variety 9311 by mRNA differential display. The transcript was increased 6 h after sprayed with the safener solution, and 4 days later, the expression still could be detected. The fragment was recycled from the polygel and sequenced, and homologous analysis revealed the cDNA was 100% identical to some ESTs and cDNAs in rice database, and the amino acid sequence was 60-84% homologous to those of the Yippee genes in several eukaryotes. The fragment was extended to the whole long cDNA, and thus a primer pair was designed. RT-PCR analysis for the designed primer supported the induction result.

关键词： rice chemical induction cDNA mRNA differential display herbicide safener inducible gene

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