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induced pib expression.and Resistance to Magnaporthe grisea are Compromised by Cytosine Demethylation at Critical Promoter Regions in Rice

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Journal of Integrative Plant Biology 2011年第10期53卷 814-823页

作者： Yuan Li Qiong Xia Hongping Kou Dan Wang Xiuyun Lin Ying Wu Chunming Xu Shaochen Xing Bao Liu Key Laboratory of Molecular Epigenetics of MOE and The Institute of Genetics ＆ Cytology Northeast Normal University Changchun 130024 China Department of Agronomy Jilin Agricultural University Changchun 130118 China Center of Agribiotechnology Jilin Academy of Agricultural Sciences Changchun 130033 China

pib is a well-characterized rice blast-resistance gene belonging to the nucleotide binding site （NBS） and leucine-rich repeat （LRR） superfamily. expression.of pib was low under non-challenged conditions, but strongly induced by the blast-causing fungal pathogen Magnaporthe grisea, thereby conferring resistance to the pathogen. It is generally established that cytosine methylation of the promoter-region often plays a repressive role in modulating expression.of the gene in question. We report here that two critical regions of the pib promoter were heavily CG cytosine-methylated in both cultivars studied. Surprisingly, induced expression.of pib by M. grisea infection did not entail its promoter demethylation, and partial demethylation by 5-azacytidine-treatment actually reduced pib expression.relative to wild- type plants. Accordingly, the blast disease-resistance was compromised in the 5＇-azaC-treated plants relative to wild-type. In contrast, the disease susceptibility was not affected by the 5＇-azaC treatment in another two rice cultivars that did not contain the pib gene, ruling out effects of other R genes and *** genotoxic effects by the drug-treatment as a cause for the compromised pib-conditioned blast-resistance. Taken together, our results suggest that promoter DNA methylation plays a novel enhancing role in conditioning high-level of induced expression.of the pib gene in times of M. grisea infection, and its conferred resistance to the pathogen.

关键词： DNA methylation Magnaporthe grisea blast-resistance induced pib expression.

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