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Characterization of SARS-COV-2 main protease inhibitory peptides from Ulva prolifera proteins

Journal of Oceanology and Limnology 2023年第5期41卷 1887-1899页

作者： Zhiyong LI Yehua WANG Caiwei FU Dongren ZHANG Tuanjie CHE Songdong SHEN School of Biology&Basic Medical Sciences Soochow UniversitySuzhou 215101China Key Laboratory of Functional Genomic and Molecular Diagnosis of Gansu Province Lanzhou 730030China

The main protease(M^(pro))is essential for the replication of SARS-COV-2 and therefore represents a promising anti-viral *** this study,we screened M^(pro)inhibitory peptides from Ulva prolifera protein on in-silico *** analysis using the online toxic prediction tool ToxinPred revealed that all the peptides were *** hexapeptide(SSGFID)exhibited high M^(pro)inhibitory activity in molecular docking and its IC_(50)value was 139.40±0.82μmol/L in vitro according to fluorescence resonance energy transfer assay(FRET).Quantitative real-time(qRT-)PCR results show that SSGFID could stimulate the expression of mitosis-related factors,including nuclear factor-κB,cyclin D1,and cyclin-dependent kinase 4,to promote the proliferation of mice *** study revealed that SSGFID showed resistance against pepsin and trypsin but lost D(Asp)after pretreatment at121℃ for 15 ***,SSGFID was mainly transported through the Caco-2 cell monolayer by the peptide transporter PepT1 and passive-mediated transport during the transport ***,the peptide was also degraded by Caco-2 intracellular enzymes,and the transfer rate of intact peptide was4.2%.Furthermore,Lineweaver–Burk plots demonstrated that SSGFID possessed a mixed inhibitory characteristic with M^(pro).Our study indicated the potential of Ulva prolifera as antiviral and immuneenhancing functional food ingredients and nutraceuticals.

关键词： M^(pro)inhibitory peptide Ulva prolifera protein in-silico proteolysis immunomodulatory peptide gastrointestinal digestion transport study

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