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in-silico analysis &In-Vivo Results Concur on Glutathione Depletion in Glyphosate Resistant GMO Soy, Advancing a Systems Biology Framework for Safety Assessment of GMOs

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American Journal of Plant Sciences 2016年第12期7卷 1571-1589页

作者： V. A. Shiva Ayyadurai Michael Hansen John Fagan Prabhakar Deonikar Systems Biology Group International Center for Integrative Systems Cambridge MA USA Consumers Union Yonkers NY USA Earth Open Source Institute Fairfield IA USA

This study advances previous efforts towards development of computational systems biology, in silico, methods for biosafety assessment of genetically modified organisms (GMOs). C1 metabolism is a critical molecular system in plants, fungi, and bacteria. In our previous research, critical molecular systems of C1 metabolism were identified and modeled using CytoSolve?, a platform for in silico analysis. In addition, multiple exogenous molecular systems affecting C1 metabolism such as oxidative stress, shikimic acid metabolism, glutathione biosynthesis, etc. were identified. Subsequent research expanded the C1 metabolism computational models to integrate oxidative stress, suggesting glutathione (GSH) depletion. Recent integration of data from the EPSPS genetic modification of Soy, also known as Roundup Ready Soy (RRS), with C1 metabolism predicts similar GSH depletion and HCHO accumulation in RRS. The research herein incorporates molecular systems of glutathione biosynthesis and glyphosate catabolism to expand the extant in silico models of C1 metabolism. The in silico results predict that Organic Soy will have a nearly 250% greater ratio of GSH and GSSG, a measure of glutathione levels, than in RRS that are glyphosate-treated glyphosate-resistant Soy versus the Organic Soy. These predictions also concur with in vivo greenhouse results. This concurrence suggests that these in silico models of C1 metabolism may provide a viable and validated platform for biosafety assessment of GMOs, and aid in selecting rational criteria for informing in vitro and in vivo efforts to more accurately decide in the problem formulation phase whose parameters need to be assessed so that conclusion on “substantial equivalence” or material difference of a GMO and its non-GMO counterpart can be drawn on a well-grounded basis.

关键词： Glutathione Genetic Modification C1 Metabolism Biomarker Safety Assessment in-silico analysis Glyphosate CytoSolve Systems Biology Safety Assessment

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Novel Point Mutations in 3'-Untranslated Region of GATA4 Gene Are Associated with Sporadic Non-syndromic Atrial and Ventricular Septal Defects

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Current Medical Science 2022年第1期42卷 129-143页

作者： Mehri KHATAMI Sajedeh GHORBANI Mojgan Rezaii ADRIANI Sahar BAHALOO Mehri Azami NAEINI Mohammad Mehdi HEIDARI Mehdi HADADZADEH Department of Biology Faculty of ScienceYazd UniversityYazd ***Iran Department of Cardiac Surgery Afshar HospitalShahid Sadoughi University of Medical SciencesYazd ***Iran

Objective:Transcription factor GATA4 has significant roles in embryonic heart *** of GATA4 appear to be responsible for a wide variety of congenital heart defects(CHD).Despite the high prevalence of GATA4 mutations in CHD phenotypes,extensive studies have not been *** 3'-untranslated region(3'-UTR)of tho GATA4 gene comprises regulatory motifs and microRNA binding sites that are critical for the appropriate gene expression,nuclear transportation,and regulation of translation,and stability of *** study aimed to evaluate the association between mutations in the 3'-UTR of the GATA4 gene and CHD risk among Iranian patients.

关键词： congenital heart disease GATA4 S'-untranslated region mutation in-silico analysis

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Contribution of Genomics to Gene Discovery in Plant Abiotic Stress Responses

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Molecular Plant 2012年第6期5卷 1176-1178页

作者： Yun Ma Feng Qin Lam-Son Phan Tran Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing100039Chlna Signaling Pathway Research Unit Plant Science CenterRIKEN Yokohama Institute1-7-22Suehiro-choTsurumiYokohama 230-0045Japan

Sustaining agricultural production under adverse environ- mental conditions, such as drought and high salinity, rep- resents a major challenge. The discovery of key genes and signal transduction pathways underlying plant responses to environmental stress will play an important role in devel- oping strategies for the genetic improvement of crops to address this challenge. Crop functional genomics has greatly contributed to the identification of abiotic stress-related genes. Current advances in genomic technologies now pro- vide effective and high-throughput methods for identifying stress-related genes at a genome-wide level, especially with the availability of the complete genomic sequence of several model and crop plant species. The development of genetic database resources has allowed bioinformatic approaches to identify stress-tolerant gene families across species based on homology and synteny. Additionally, genome-wide associa- tion studies （GWAS） for complex trait loci in crops have facili- tated the discovery of critical stress-related genes and their favorable alleles.

关键词： in-silico analysis TRANSCRIPTION FACTORS DEHYDRATION STRESS PREDICTION

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