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*** imaging of retinal pericytes:evidence for early calcium uptake,capillary constriction and vascular dysregulation in ocular hypertension glaucoma

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Annals of Eye Science 2018年第1期 417-417页

作者： Luis Alarcon-Martinez Jorge L.Cueva Vargas Nicolas Belforte Deborah Villafranca-Baughman Adriana Di Polo Department of Neuroscience University of Montreal Hospital Research Center(CR-CHUM)MontrealQuebecCanada

Background:Pericytes are contractile cells that wrap along the walls of *** the brain,pericytes play a crucial role in the regulation of capillary diameter and vascular blood flow in response to metabolic *** contribution of pericytes to microvascular deficits in glaucoma is currently *** address this,we used two-photon excitation microscopy for longitudinal monitoring of retinal pericytes and capillaries in a mouse glaucoma ***:Ocular hypertension was induced by injection of magnetic microbeads into the anterior chamber of albino mice expressing red fluorescent protein selectively in pericytes(NG2-DsRed).Minimally invasive,multiphoton imaging through the sclera of live NG2-DsRed mice was used to visualize pericytes and capillary diameter at one,two and three weeks after glaucoma *** vivo fluctuations in pericyte intracellular calcium were monitored with the calcium indicator *** vivo stereological analysis of retinal tissue prior to and after injection of microbeads was used to confirm our in vivo ***:Live two-photon imaging of NG2-DsRed retinas demonstrated that ocular hypertension induced progressive accumulation of intracellular calcium in *** uptake correlated directly with the narrowing of capillaries in the superficial,inner,and outer vascular plexuses(capillary diameter:naïve control=4.7±0.1μm,glaucoma=4.0±0.1μm,n=5-6 mice/group,Student’s t-test Ptwo main ***,two-photon excitation microscopy is an effective strategy to monitor longitudinal changes in retinal pericytes and capillaries in live animals at glaucoma onset and ***,ocular hypertension triggers rapid int

关键词： Glaucoma pericytes capillaries in vivo two-photon microscopy

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*** on microvessels lead to vascular dysfunction during retinal ischemia

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Annals of Eye Science 2018年第1期 448-448页

作者： Deborah Villafranca-Baughman Luis Alarcón-Martínez Adriana Di Polo Department of Neuroscience University of Montreal Hospital Research Center(CR-CHUM)MontrealQuebecCanada

Background:Pericytes are contractile cells that wrap along the walls of *** the brain,pericytes play a crucial role in the regulation of capillary diameter and vascular blood flow in response to metabolic *** ischemia,it has been suggested that pericytes may constrict capillaries,and that pericytes remain constricted after reperfusion thus resulting in impaired blood ***:Here,we used a mouse model of retinal ischemia based on ligation of the central retinal artery to characterize the role of pericytes on capillary *** was induced in transgenic mice carrying the NG2 promoter driving red fluorescent protein expression to selectively visualize pericytes(line NG2:DsRed).Changes in retinal capillary diameter at 1 hr after ischemia were measured ex vivo in whole-mounted retinas from ischemic and control eyes(n=4-6/group)using a stereological *** and pericytes were three-dimensionally reconstructed using IMARIS(Bitplane).Furthermore,we used a novel and minimally invasive two-photon microscopy approach that allowed live imaging of microvasculature changes in the ***:Our data show a generalized reduction in capillary diameter in ischemic retinas relative to sham-operated controls in all vascular plexus(ischemia:4.7±0.2μm,control:5.2±0.2µm,student’s t-test,Ptwo-photon microscopy confirmed robust capillary constriction at the level of pericytes on retinal capillaries during ischemia(n=6-8/group).Conclusions:Collectively,our data demonstrate that ischemia promotes rapid pericyte constriction on retinal capillaries causi

关键词： Ischemia pericyte blood flow regulation in vivo two-photon microscopy

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