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Dental pulp stem cells express tendon markers under mechanical loading and are a potential cell source for tissue engineering of tendon-like tissue

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International Journal of Oral Science 2016年第4期8卷 213-222页

作者： Yu-Ying Chen Sheng-Teng He Fu-Hua Yan Peng-Fei Zhou Kai Luo Yan-Ding Zhang Yin Xiao Min-Kui Lin Department of Stomatology Fujian Provincial Hospital School and Hospital of Stomatology Fujian Medical University Department of Stomatology Hainan Province Nongken Sanya Hospital Nanjing Stomatological Hospital Medical School of Nanjing University Australia-China Centre for Tissue Engineering and Regenerative Medicine Queensland University of Technology College of Life Science Fujian Normal University Institute of Health and Biomedical Innovation Queensland University of Technology

Postnatal mesenchymal stem cells have the capacity to differentiate into multiple cell lineages. This study explored the possibility of dental pulp stem cells （DPSCs） for potential application in tendon tissue engineering. The expression of tendon- related markers such as scleraxis, tenascin-C, tenomodulin, eye absent homologue 2, collagens I and VI was detected in dental pulp tissue. Interestingly, under mechanical stimulation, these tendon-related markers were significantly enhanced when DPSCs were seeded in aligned polyglycolic acid （PGA） fibre scaffolds. Furthermore, mature tendon-like tissue was formed after transplantation of DPSC-PGA constructs under mechanical loading conditions in a mouse model. This study demonstrates that DPSCs could be a ootential stem cell source for tissue enEineerin~ of tendon-like tissue.

关键词： dental pulp stem cells in vivo model mechanical loading tendon engineering

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SOCS1/JAK2/STAT3 axis regulates early brain injury induced by subarachnoid hemorrhage via inflammatory responses

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Neural Regeneration Research 2021年第12期16卷 2453-2464页

作者： Yang Wang Xiang-Qian Kong Fei Wu Bin Xu De-Jun Bao Chuan-Dong Cheng Xiang-Ping Wei Yong-Fei Dong Chao-Shi Niu Department of Neurosurgery First Affiliated Hospital of University of Science and Technology of ChinaDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Department of Vascular Surgery Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinanShandong ProvinceChina Department of Vascular Surgery Shandong Provincial HospitalCheeloo College of MedicineShandong UniversityJinanShandong ProvinceChina Anhui Medical College Anhui Provincial Medical Genetics CenterHefeiAnhui ProvinceChina Anhui Province Key Laboratory of Brain Function and Brain Disease HefeiAnhui ProvinceChina

The SOCS1/JAK2/STAT3 axis is strongly associated with tumor growth and progression,and participates in cytokine secretion in many diseases.However,the effects of the SOCS1/JAK2/STAT3 axis in experimental subarachnoid hemorrhage remain to be studied.A subarachnoid hemorrhage model was established in rats by infusing autologous blood into the optic chiasm pool.Some rats were first treated with JAK2/STAT3 small interfering RNA(Si-JAK2/Si-STAT3)or overexpression plasmids of JAK2/STAT3.In the brains of subarachnoid hemorrhage model rats,the expression levels of both JAK2 and STAT3 were upregulated and the expression of SOCS1 was downregulated,reaching a peak at 48 hours after injury.Simultaneously,the interactions between JAK2 and SOCS1 were reduced.In contrast,the interactions between JAK2 and STAT3 were markedly enhanced.Si-JAK2 and Si-STAT3 treatment alleviated cortical neuronal cell apoptosis and necrosis,destruction of the blood-brain barrier,brain edema,and cognitive functional impairment after subarachnoid hemorrhage.This was accompanied by decreased phosphorylation of JAK2 and STAT3 protein,decreased total levels of JAK2 and STAT3 protein,and increased SOCS1 protein expression.However,overexpression of JAK2 and STAT3 exerted opposite effects,aggravating subarachnoid hemorrhage-induced early brain injury.Si-JAK2 and Si-STAT3 inhibited M1-type microglial conversion and the release of pro-inflammatory factors(inducible nitric oxide synthase,interleukin-1β,and tumor necrosis factor-α)and increased the release of anti-inflammatory factors(arginase-1,interleukin-10,and interleukin-4).Furthermore,primary neurons stimulated with oxyhemoglobin were used to simulate subarachnoid hemorrhage in vitro,and the JAK2 inhibitor AG490 was used as an intervention.The in vitro results also suggested that neuronal protection is mediated by the inhibition of JAK2 and STAT3 expression.Together,our findings indicate that the SOCS1/JAK2/STAT3 axis contributes to early brain injury after subarachnoid hemorrhage both in vitro and in viv

关键词： brain injury cytokines in vitro model in vivo model inflammation microglia SOCS1/JAK2/STAT3 axis subarachnoid hemorrhage

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Safety and biodegradability of a synthetic dural sealant patch(Liqoseal)in a porcine cranial model

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Animal models and Experimental Medicine 2021年第4期4卷 398-405页

作者： Ahmet Kinaci Wilhelmina Bergmann Sander van Thoor Saskia Redegeld Albert van der Zwan Tristan P.C.van Doormaal Department of Neurology and Neurosurgery Brain CenterUniversity Medical Centre UtrechtUtrechtThe Netherlands Brain Technology Institute UtrechtThe Netherlands Division of Pathology Faculty of Veterinary MedicineUtrecht UniversityUtrechtThe Netherlands Department of Neurosurgery Clinical Neuroscience CenterUniversity Hospital ZurichZurichSwitzerland

Background:Liqoseal consists of a watertight layer of poly(ester)ether urethane and an adhesive layer containing polyethylene glycol-N-hydroxysuccinimide(PEG-NHS).It is designed to prevent cerebrospinal fluid(CSF)leakage after intradural surgery.This study assessed the safety and biodegradability of Liqoseal in a porcine craniotomy model.Methods:In 32 pigs a craniotomy plus durotomy was performed.In 15 pigs Liqoseal was implanted,in 11 control pigs no sealant was implanted and in 6 control pigs a control dural sealant(Duraseal or Tachosil)was implanted.The safety of Liqoseal was evaluated by clinical,MRI and histological assessment.The degradation of Liqoseal was histologically estimated.Results:Liqoseal,2 mm thick before application,did not swell and significantly was at maximum mean thickness of 2.14(±0.37)mm at one month.The foreign body reaction induced by Liqoseal,Duraseal and Tachosil were comparable.Liqoseal showed no adherence to the arachnoid layer and was completely resorbed between 6 and 12 months postoperatively.In one animal with Liqoseal,an epidural fluid collection containing CSF could not be excluded.Conclusion:Liqoseal seems to be safe for intracranial use and is biodegradable.The safety and performance in humans needs to be further assessed in clinical trials.

关键词： cerebrospinal fluid leakage craniotomy dura in vivo model sealant

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