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Change of extracellular ascorbic acid in the brain cortex following ice water vestibular stimulation： an on-line electrochemical detection coupled with in vivo microdialysis sampling for guinea pigs

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Chinese Medical Journal 2008年第12期121卷 1120-1125页

作者： ZHANG Na LIU Jun-xiu MA Fu-rong YU Li-sheng LIN Yu-qing LIU Kun MAO Lan-qun Department of Otorhinolaryngology Peking University Third Hospital Beijing 100083 China Department of Otorhinolaryngology Peking University People's Hospital Beijing 100044 China Beijing National Laboratory for Molecular Sciences Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China

Background Ascorbic acid （AA） represents one of the most important enzyme co-factors, antioxidants and neuromodulators and plays an important role in the cerebral system. Increasing evidence has suggested that AA could treat certain kinds of vertigo diseases such as Meniere＇s disease. To elucidate the neurochemical functions associated with AA in vertigo, the change of extracellular AA in the brain cortex following caloric vestibular stimulation （CVS） was evaluated.Methods An on-line electrochemical detection was coupled with in vivo microdialysis to continuously monitor the change of extracellular AA in the primary somatosensory （SI） area of guinea pigs following a caloric vestibular stimulation. Sixteen guinea pigs were divided into three groups, i.e., experimental group with irrigation of the ear canal with ice water （0℃）（n=-8）, and two control groups, one with irrigation of the ear canal with warm water （38℃）（n=-4） and the other with irrigation of the auricle with ice water （n=-4）.Results In the experimental group, the ice water irrigation of the left external ear canal induced a horizontal nystagmus towards the right side lasting about 45 seconds. No nystagmus was induced by warm water irrigation of the external ear canal or by ice water irrigation of the auricle. The extracellular AA concentration significantly increased following the ice water vestibular stimulation, reaching a maximum of （130±20）% （n=8） of the basal dialysate level （2.61±0.92） μmol/L （n=8）, lasting at least for an hour. AA level did not change distinctly after the irrigation of the left external ear canal with warm water or the irriclation of the auricle with ice water.Conclusions The concentration of extracellular AA in the brain cortex of the SI area increased following the ice water vestibular stimulation. This demonstration may be useful for the investigation of the neurochemical processes associated with AA in the process of vertigo.

关键词： ascorbic acid caloric vestibular stimulation primary somatosensory area in vivo microdialysis on-line electrochemical detection

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In vivo electrochemical recording of continuous change of magnesium in medial vestibular nucleus following vertigo induced by ice water vestibular stimulation

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Science China Chemistry 2013年第2期56卷 256-261页

作者： XIN Ying ZHANG ZiPin YU Ping MA FuRong MAO LanQun Department of Otorhinolaryngology Peking University Third Hospital Beijing National Laboratory for Molecular Sciences Key Laboratory of Analytical Chemistry for Living Biosystems Institute of Chemistry Chinese Academy of Sciences

Vertigo is one of the most common clinical symptoms. However, the chemical processes involved in the pathological mechanism of vertigo remain to be fully understood. In this study, we investigate the dynamic changes in the magnesium (Mg 2+ ) concentration in medial vestibular nucleus (MVN) of guinea pigs following vertigo induced by vestibular ice water stimulation with an electrochemical detection method consisting of in vivo microdialysis and on-line selective electrochemical detection. Electrochemical detection of Mg 2+ was accomplished based on the current enhancement of Mg 2+ towards the electrocatalytic oxidation of NADH at the electrodes modified with the polymerized film of toluidine blue O (TBO). Selectivity for the on-line electrochemical detection against Ca 2+ was achieved by using ethyleneglcol-bis(2-aminoethylether) tetraacetic acid (EGTA) as the selective masking agent for Ca 2+ . The basal level of the extracellular Mg 2+ in the MVN of guinea pigs was determined to be 759.7 ± 176.2 M(n = 16). Upon ice water irrigation of the left external ear canal, the concentration of Mg 2+ in the MVN decreases significantly, reaches 72 ± 6% (n = 8) of the basal level, and maintains for at least 1000 s. Control experiments reveal that neither warm water irrigation of the external ear canal nor ice water irrigation of the auricle induces the decrease in the concentration of Mg 2+ in the MVN. These results demonstrate that the extracellular Mg 2+ in the MVN decreases significantly following vertigo induced by vestibular ice water stimulation. This demonstration suggests that Mg 2+ might play an important role in the pathological mechanism of vertigo.

关键词： on-line electrochemical detection in vivo microdialysis magnesium vertigo

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Extracellular ascorbic acid fluctuation during the protective process of ischemic preconditioning in rabbit renal ischemia-reperfusion model measured

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Chinese Medical Journal 2010年第11期123卷 1441-1446页

作者： LIU Lei LIN Yu-qing YAN Long-tao HONG Kai HOU Xiao-fei MAO Lan-qun MA Lu-lin Department of Urology Peking University Third Hospital Beijing100191 China Institute of Cllemistry Chinese Academy of Sciences Beijing100190 China

Background Ascorbic acid has important antioxidant ischemic preconditioning on later ischemia-reperfusion ascorbic acid in ischemic preconditioning in the kidney. properties, and may play a role in the protective effects of Herein, we examined the role of endogenous extracellular Methods We developed a solitary rabbit kidney model where animals received ischemia-reperfusion only （ischemia-reperfusion group, n=-15） or ischemic preconditioning followed by ischemia-reperfusion （ischemic preconditioning group, n=15）. Ischemia-reperfusion was induced by occluding and loosening of the renal pedicle. The process of ischemic preconditioning included 15-minute brief ischemia and 10-minute reperfusion. In vivo microdialysis coupled with online electrochemical detection was used to determine levels of endogenous extracellular ascorbic acid in both groups. The extent of tissue damage was determined in kidney sections stained with hematoxylin and eosin. Serum creatinine and urea nitrogen were also detected to assess renal function. Results During ischemia-reperfusion, the extracellular ascorbic acid concentration during ischemia increased rapidly to the peak level （（130.01±9.98）%）, and then decreased slowly to near basal levels. Similar changes were observed during reperfusion （peak level, （126.78±18.24）%）. In the ischemic preconditioning group there was a similar pattern of extracellular ascorbic acid concentration during ischemic preconditioning. However, the ascorbic acid level was significantly lower during the ischemia and early reperfusion stage compared to the ischemia-reperfusion group. Additionally, the extent of glomerular ischemic collapse, tubular dilation, tubular denudation, and loss of brush border were markedly attenuated in the ischemic preconditioning group. Levels of serum creatinine and urea nitrogen were also decreased significantly in the ischemic preconditioning group. Conclusions Ischemic preconditioning may protect renal tissue against ischemia-reperfusi

关键词： in vivo microdialysis online electrochemical detection, ascorbic acid, renal ischemia-reperfusion ischemic preconditioning

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5-HT and 5-HT1A receptor in the hippocampal DG region are involved in the active avoidance learning in rats

5-HT and 5-HT1A receptor in the hippocampal DG region are in...

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中国神经科学学会第十届全国学术会议

作者： Feng-Ze JIANG Shi WANG Peng WAN Jing LV Qing-Hua JIN Department of Physiology and Pathophysiology Yanbian University Medical College

Learning and memory are major functions of the high levels of the nervous system,and the association between these functions and neurotransmitter functions is of great interest for understanding brain ***（5-HT） is a classical neurotransmitter,and serotonergic pathways play an important role in the modulation of learning and memory,but the importance of its receptor types and subtypes on learning and memory is still *** the5-HT receptors subtypes,5-HT1A receptors are of special interest because these receptors are present in the brain areas involved in learning and memory such as *** present study was therefore designed to investigate the effect of activation and blockade of 5-HT1A receptor on learning and memory in rats using shuttle ***,the extracellular concentrations of 5-HT in the hippocampal DG region were measured by in vivo microdialyais technique during the active avoidance task in conscious ***,WAY-100635（an antagonist of 5-HT1A receptor） and8-OH-DPAT（an agonist of 5-HT1A receptor） were locally microinjected into the hippocampal DG region before the avoidance training,and then the rate of avoidance was observed.

关键词： hippocampal DG region 5-HT 5-HT1 receptor active avoidance learning conscious rats in vivo microdialysis

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Temporal changes of Glutamate release in the medial vestibular nucleus following unilateral laby-rinthectomy in conscious rats

Temporal changes of Glutamate release in the medial vestibul...

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中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：刘思源卢焕俊金元哲李香兰 Department of Physiology and pathophysiology Yanbian University College of Medicine

To understand the neurochemical mechanism underlying the vestibular compensation,we determined the levels of glutamate following unilateral labyrinthectomy(UL)by using in vivo brain microdialysis and high-performance liquid chromatography(HPLC)*** were pretreated by infusing 10 mg arsanilic acid into the tympanic cavity to obstruct uni-periphery vestibular organ,and then the changes of glutamate release from the ipsi-and contra-lesional medial vestibular nucleus(MVN)following UL were investigated in conscious *** concentration of glutamate in the ipsi-lesional MVN,which increased until UL 12h,after which the release did not show any change between 12 and 48 h post-UL,decreased gradually to the basal level until 48-144 h post-UL followed by no further *** contrast,the concentration of glutamate in the contra-lesional MVN,which increased immediately after UL 2 h,decreased gradually to the basal level until 6-12 h post-UL,after which the release did not show any change between 12 and 24 h post-UL,and then increased significantly between 24 and 72 h post-UL,decreased gradually to the basal level until 72-144 h post-UL followed by no further *** in the glutamate concentration between ipsi-and contra-lesional MVN increased immediately after UL and gradually decreased accompanied by a reduction in the frequency of *** result suggest that the imbalance of glutamate release between bilateral nuclei induced the nystagmus,and the change in release is concerned with the rapid development of vestibular compensation.

关键词： Glutamate Medial vestibular nucleus in vivo microdialysis Unilateral labyrinthectomy Vestibular compensation

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