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Aqueous-phase synthesis of upconversion metal-organic frameworks for ATP-responsive in situ imaging and targeted combinational cancer therapy

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Chinese Chemical Letters 2022年第1期33卷 314-319页

作者： Lin Yang Shuaidong Zhu Zhimei He Xiangli Li Jiangning Chen Sai Bi Jun-Jie Zhu State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringSchool of Life SciencesNanjing UniversityNanjing 210023China College of Chemistry and Chemical Engineering Qingdao UniversityQingdao 266071China

Herein,the nanoscaled ATP-responsive upconversion metal-organic frameworks(UCMOFs)are aqueousphase synthesized for co-delivery of therapeutic protein cytochrome c(Cyt c)and chemodrugs doxorubicin(DOX),achieving targeted combinational therapy of human cervical cancer.The UCMOFs are rationally fabricated by growing ZIF-90 on mesoporous silica-coated upconversion nanoparticles(UCNPs),in which the ZIF-90 layer attenuates the upconversion luminescence(UCL)and the rigid frameworks increase the stability of encapsulated proteins.Once the UCMOF@DOX/Cyt c are internalized into HeLa cells via specific recognition of sgc8 aptamers,the intracellular ATP triggers the dissolution of ZIF-90 into Zn^(2+),which facilitates not only the release of Cyt c and DOX but also the restoration of UCL for real-time monitoring of drug release.It has been demonstrated that the therapeutic efficacy is greatly improved by the combination of caspase-mediated apoptosis activated by Cyt c(protein therapeutics),DNA fragmentation induced by DOX(chemotherapy),and Zn;-promoted generation of reactive oxygen species(ROS)(oxidative stress).Overall,our proposed multifunctional UCMOFs provide an effective platform for targeted combinational cancer therapy and in situ imaging,which hold great promise in biomedical and clinical applications.

关键词： Upconversion nanoparticles Metal-organic frameworks ATP response in situ imaging Targeted combinational therapy

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A controllable self-localized imaging strategy capable of synchronous in situ tracking of local changes in intracellular bioactive small-molecules

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Science China Chemistry 2023年第8期66卷 2425-2434页

作者： Ke Li Shuai Xu Benhua Wang Shuangyan Huan Xiangzhi Song Lin Yuan Xiao-Bing Zhang State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha410082China College of Chemistry&Chemical Engineering Central South UniversityChangsha410083China School of Chemistry and Chemical Engineering University of JinanJinan250022China

In situ tracking and localization of ubiquitous bioactive small molecules(BSMs)within their native habitats is particularly challenging because of their low-molecular weight and widespread distribution properties.We report the proof of concept of a synchronous in situ imaging strategy,whereby the representative BSM amino-biothiols(ABs)mediate activation of the selflocalizable probe HYPQS,thereby releasing insoluble emissive precipitates to afford holistic distribution information of ABs.Notably,three organelle-targetable ABs inhibitors were innovatively fabricated for directed clearance of ABs in particular organelles,providing a powerful aid for HYPQS to achieve programmed in situ tracking of ABs in different organelles“on demand”.Biological transmission electron microscopy images confirmed that this probe released insoluble emissive precipitates at the reaction sites,which is of primary importance for achieving synchronous in situ tracking of BSMs.Furthermore,the probe HYPQS was successfully applied to monitor the dynamic changes in the endogenous ABs pool during diverse cell events.This strategy opens a promising avenue for investigating the undiscovered functional mechanism of local BSMs in relevant biological processes.

关键词： bioactive small-molecules in situ imaging fluorescent probes precipitated fluorophore imaging reagents

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Tracking of pesticides on/inside vegetable leaves by in situ SERS imaging

Tracking of pesticides on/inside vegetable leaves by in situ...

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第二十届全国光散射学术会议（CNCLS20）

作者： Feng Wang Huichao Fang Haifeng Yang Department of Chemistry Shanghai Normal University

Pesticide residues are harmful to human health.However,most present techniques that can be used for the detection and identification of pesticides are time consuming,and cannot be adopt for in situ and real time detection.In this study,we applied in situ SERS technique to detect and discriminate pesticides on/inside vegetable leaves in a rapid,sensitive way.AuNPs suspension was dropped onto vegetable leaves.AuNPs with a diameter of 40 nm can penetrate as deep as 300 μm after 12 hr.Pesticide was then applied on vegetable leaves pre-penetrated with AuNPs（Figure 1）,Z-stack SERS imaging results proved the feasibility of in situ SERS imaging technique for pesticide penetration study.In situ SERS imaging was then applied to study the pesticide removing effect of different washing reagents on/inside vegetable leavess（Figure 2）.

关键词： SERS in situ imaging pesticide vegetable leaves

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Activation of Fluorescent Probe Releasing a Precipitating Fluorochrome in situ Localization imaging of Furin in Live Cells and Tissues

Activation of Fluorescent Probe Releasing a Precipitating Fl...

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中国化学会第十三届全国分析化学年会

作者： Hong-Wen Liu Ke Li Xiao-Bing Zhang Molecular Science and Biomedicine Laboratory （MBL） State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering College of Life Sciences Hunan University

Furin, a kind of trans-Golgi proprotein convertases, plays important roles in many physiological processes and is overexpressed in inflammatory conditions and cancer cells.1 in situ imaging furin is a significant job for accurately monitoring the diseases and timely treatment. However, traditional fluorogenic probes can’t provide with in situ information, because the released fluorophores are always easily diffuse away from the reaction sites, resulting in dramatically reducing spatial resolution and thus negative signal.2 6-chloro-2-（2-hydroxyphenyl） quinazolin-4（3H）-one（HPQ）, a solid-state fluorophore, is strongly hydrophobic and can easily precipited at the reaction sites to avoid signal diffusing away. In this work, we developed a probe HPQF for in situ and long-term imaging of fruin activity in live cells by integrating the HPQ and furin specific peptide substrate through a self-immolative linker. In situ detection and imaging is significant to obtain real information of furin activities. And it is may facilitate the study of furin function in tumorigenicity and the discovery of new drugs for furin-targeted cancer therapy.

关键词： furin HPQ in situ imaging real time

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