检索结果-南通市图书馆

in vitro culture of immature embryos from Koelreuteria bipinnata var. integrifoliola

在线全文

学校读者我要写书评

暂无评论

Forestry Studies in China 2009年第3期11卷 179-184页

作者： FENG Da-ling ZHANG Jie LIU Xia PENG Wei-xiu WU Tong-yan College of Life Science Agricultural University of Hebei Baoding 071001 E R. China College of Landscape Architecture and Tourism Agricultural University of Hebei Baoding 071001 P. R. China

For the mass production of Koelreuteria bipinnata var. integrifoliola with selected, hybrid or genetically engineered genotypes, one potentially desirable propagation strategy is based on embryo culture. The immature embryo development in vitro from K. bipinnata var. integrifoliola was studied under different conditions of embryo age, basic culture media and plant growth regulators. The results show that： 1） germination rate of grade 3 embryos in immature seeds with 0.6-0.8 cm diameter was 98.9%. The germination rate of grade 2 embryos in immature seeds with 0.4-0.6 cm diameter was 77,8% and the germination rate of grade 1 embryos in immature seeds with 0.4 cm diameter was 15.6%. 2） The amounts of macroelements in MS medium had no clear effect on the germination rate of immature grade 3 embryos and had a modest effect on plantlet growth, where the best medium was MS or 1/2 MS. The rates were all greater than 90%. 3） The germination rate of grade 3 embryos was greater than 87% when the medium contained a low concentration of NAA or no plant growth regulators at all and decreased markedly when BAP alone or BAP and NAA together were added to the media. We suggest that in vitro culture of immature embryos from K. bipinnata vat. integrifoliola can be enhanced when a small amount of plant growth regulators is added. The addition of BAP has an adverse reaction to the germination and development of immature embryos.

关键词： Koelreuteria bipinnata var. integrifoliola immature embryo in vitro culture germination rate

in vitro culture of Unfertilized Ovary of Strawberry(Fragaria spp.)

在线全文

学校读者我要写书评

暂无评论

Agricultural Science & Technology 2011年第12期12卷 1790-1794,1856页

作者：王文和吴禄平赵玉萍北京农学院园林学院北京102206 沈阳农业大学园艺学院辽宁沈阳110161

[Objective] This study was to investigate the in vitro culture of unfertilized ovary of strawberry.[Methods] Employing single factor experiment,we investigated six key factors including genotype,extrinsic hormone,sucrose concentration,low temperature pretreatment,growth environment and development status,and illumination condition on induction of gynogenesis in vitro of unfertilized ovary,on the induction of gynogenesis in vitro of unfertilized ovary.[Results] The optimal conditions for in vitro culture of unfertilized ovary of strawberry were as follows：the primary flower buds cultured on bare land as explants,selection of appropriate genotype,2,4-D as external hormone,sucrose at the concentration of 6%,low temperature pretreatment for 48 hours and dark culture under alternated temperature.[Conclusion] The research provided reference for ploidy breeding in strawberry.

关键词： Fragaria spp. Unfertilized ovary Gynogenesis in vitro culture

in vitro culture of Early Rabbit Embryos Injected with Foreign Gene and Its Retention

在线全文

学校读者我要写书评

暂无评论

Developmental and Reproductive Biology 1993年第2期2卷 62-68,T001页

作者：陈请轩刘雷邓辉南宋德秀 Institute of Developmental Biology Academia Sinica Beijing 100080

Fertilized rabbit eggs injected with SMTPGH gene were cultured in vitro and retention of theinjected gene was studied using PCR technique and nonradioactive labelling methed. In a mediumof TC199 + 10% FCS, three quarters of the fertilized eggs developed to the blastocyst stage. Noapparent change of the injected gene was found before the 8-cell stage, after which it was eitherintegrated into the chromosome of the host or lost gradually. But finally, the retention rate of theinjected gene should be equal to its integration rate.

关键词： Injected Embryo of Rabbit SMTPGH Gene in vitro culture

Effects of Hormone Factors on the in vitro culture Flowering Induction of Dendrobium officinate Kimura et Migo

在线全文

学校读者我要写书评

暂无评论

Agricultural Science & Technology 2010年第4期11卷 75-79页

作者：岑秀芬黄春红韦鹏霄广西大学农学院广西南宁530005

[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Kimura et Migo were used as the experimental materials and MS medium as the basic *** tests have been done between single-factor hormone treatments（different concentrations of PP333 or TDZ） and multi-factor hormone treatments（different combinations of PP333,TDZ,6-BA and NAA） to research the effects of hormone factors on the flowering induction of the plantlets.[Result]Among the single-factor hormone treatments,the suitable concentration and the rate of flower buds formation of PP333 treatment were 0.2 mg/L and 8.5%,the that of TDZ treatment were 0.06 mg/L and 15.5%;the effects of multi-factor hormone treatments on the flowering induction were ordered as follow：（PP333 ＋ 6-BA ＋ NAA ＋ TDZ）〉（PP333 ＋ 6-BA ＋ NAA）〉（PP333 ＋ 6-BA） and（PP333 ＋ NAA） ;the most suitable treatment was PP333 0.3 mg/L ＋ 6-BA 0.5 mg/L ＋ NAA 0.5 mg/L ＋ TDZ 0.06 mg/L,the rate of flower bud formation and the rate of the blossomed flower were respectly reached to 80.4% and 90.3%.[Conclusion]PP333 and TDZ showed the important effect on the flowering induction in vitro culture of Dendrobium officinate Kimura et *** effect of TDZ was better than that of *** is much more conducive to the flower bud formation,when using appropriate concentration of TDZ combined with other hormones properly.

关键词： Dendrobium officinate Kimura et Migo in vitro culture Hormone factor Flowering induction

Establishment of in vitro culture of Populus euphratica Olivier

在线全文

学校读者我要写书评

暂无评论

Forestry Studies in China 2006年第4期8卷 10-14页

作者： Zhao Peng Dong Zhan-yuan Sun Hong-bin Zhao Ju-ying Wang Hua-fang College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 P. R. China Ecology ＆ Environment College Inner Mongolia Agricultural University Huhhot 010019 P. R. China Alashan Australian Affairs Office Bayanhaote 750306 P. R. China Institute of Forestry of Alashan Inner-Mongolia Alashan 750306 E R. China

The purpose of our study was to establish a regeneration system for micropropagation of Populus euphratica Olivier. On the basis of an analysis of plant leaf mineral nutrients, a special medium was proposed, called MP2. In optimizing media for in vitro plant cultures including MS, B5 and MP2 media we employed hormones, auxin IAA, cytokine benzyladenine （BAP） and gibberellic acid （GA） in our factorial experiments on media. Adventitious shoots were derived from cuttings of adult plants taken from Xingii- ang, west China, on selected media with MP2^＋ 0.5 mg·L^-1BA＋0.1 mg·L^-1 NAA. The shoots were elongated on a medium with 0.25 mg·L^-1 BAP, 0.1 mg·L^-1NAA and 2 mg·L^-1 GA and were then rooted on a medium with 0.2-0.5 mg·L^-1 IBA. All the media were incorporated with 30 g·L^-1 sucrose and an adjusted pH at 6.3.

关键词： Populus euphratica Olivier plant hormones in vitro culture micropropagation

Preparation, cryopreservation and in vitro culture of spermatogonial stem cells of new born calves

在线全文

学校读者我要写书评

暂无评论

Journal of Agricultural Science and Technology 2009年第6期3卷 13-16,23页

作者： ZHANG Gui-xue LI Wan-hua LV Zhong-hua HU Peng-fei HUANG Zhi-jun LI Dong-xu ZHENG Peng College of Animal Science and Technology Northeast Agricultural University Harbin 150030 China

The experiment was designed to study the preparation, cryopreservation and culture of calf spermatogonial stem cells in order to provide some data for theoretical research and practical use of calf spermatogonial stem cells. As for enzymolysis of testis tissue, two digestive method A and B. The cryopreservation cooling procedure was that spermatogonial stem cells was equilibrated at 4℃ for lh, at -20℃ for lh, at -40℃ for 2h, at -80℃ overnight and then stored in liquid nitrogen. The different concentration of three eryoprotectants were compared. The results indicated that both method A and method B achieved the same high motility rates of cells with method B needing time longer, calf spermatogonial stem cells could be well cryopreserved by the stage cooling procedure. Satisfactory cryopreservative results were gotten by adding 10% DMSO or 10% EG to cryopreservative medium, the former was better and adding sucrose could improved cryopreservative effect. The culture behaviors of spermatogonial stem cells kept the same before and after cryopreservation. It was concluded that an effective method was verified for preparation, cryopreservation and culture of new calf spermatogonial stem cells.

关键词： new calf spermatogonial stem cells cryopreservation in vitro culture

Effect of pre-culture on virus elimination from in vitro apple by thermotherapy coupled with shoot tip culture

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2018年第9期17卷 2015-2023页

作者： HU Guo-jun DONG Ya-feng ZHANG Zun-ping FAN Xu-dong REN Fang LI Zheng-nan Research Institute of Pomology Chinese Academy of Agricultural Sciences

We evaluated the role of pre-culture on survival rate of in vitro apple plants treated by thermotherapy. Two apple cultivars, Malusxdomestica cv. Pink Lady and Huafu, were used in the experiment and both have widely grown in China and infected with Apple chlorotic leafspot virus （ACLSV） and Apple stem grooving virus （ASGV）. Results in growth and virus titer of apple plants did not exhibit clear trends during five different periods of pre-culture. Whilst, pre-culture increased the survival rate of the two cultivars during thermotherapy. The survival rate of plants pre-cultured for 13 d （P-13d） was 14 and 51% higher than that of P-ld plants for Pink Lady and Huafu, respectively. Moreover, pre-culture positively influenced regeneration of Huafu plants. The average survival rate of plants regenerated from P-ld and P-4d was 20% lower than that regenerated from P-7d, P-10d, and P-13d. The efficiency of virus eradication was determined by reverse-transcription PCR with two primer pairs for each virus, and the detection results showed that pre-culture scarcely affected apple virus elimination. Despite the fact that the two viruses were hardly detected at 5 d of thermotherapy, no virus-free plants were found in the two cultivars of regenerated apple plantlets after 30-d treatment.

关键词： apple in vitro culture pre-culture thermotherapy virus elimination

In vitro long-term culture and differentiation of mouse spermatogonia stem cells

在线全文

学校读者我要写书评

暂无评论

Journal of Agricultural Science and Technology 2008年第10期2卷 1-5页

作者： LANG Hong-yan ZHANG Gui-xue HUANG He LI Dong-xu HU Peng-fei College of Animal Science and Technology Northeast Agricultural University Harbin 150030 China

The studies from 7 or 8 days old mice were used to prepare the spermatogonial stem cells. The isolation and purification of spermatogonial stem cells were done by means of the Percoll discontinual density gradient concentration. The cells of the 3rd band were collected and cultured in vitro in DMEM supplemented with 2.5% or 10% fetal bovine serum （FBS）. The results showed that with the feeder layer and 2.5% serum, the spermatogonial stem cells could proliferate, differentiate last more than 4 months.

关键词： spermatogonial stem cell in vitro culture proliferation differentiation

Normal and Degenerated Rabbit Nucleus Pulposus Cells in in vitro cultures: A Biological Comparison

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Huazhong University of Science and Technology(Medical Sciences) 2013年第2期33卷 228-233页

作者：何斌王玉环杨坚彭方亮李锋 Department of Orthopedics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Department of Orthopedics the First Affiliated Hospital of Medical College of Shihezi University Medical College of Shihezi University

This study examined the biological characteristics of normal and degenerated rabbit nucleus pulposus （NP） cells in vitro in order to provide seed cells for intervertebral disc （IVD） tissue engineering. A total of 8 adult New Zealand white rabbits underwent annulus puncture to establish models ofintervertebral disc degeneration （IDD）. Four weeks later, normal and degenerated NP cells were obtained. Cell morphology was observed by light and electron microscopy. Cell viability was measured by MTT assay. Cell cycle and expression of extracellular matrix （ECM）-related genes （aggrecan and type II col- lagen） were determined by using flow cytometry and RT-PCR respectively. The growth curve of normal NP cells showed that the cells at passage 4 tended to slowly grow on the fifth day of culture. The density of normal NP cells at passages 5 to 7 was significantly less than that of the first-passage cells 2 or 3 days after seeding （P〈0.05）. The degenerated NP cells at passage 3 showed slow growth at 4th day. After 5 passages, the degenerated NP cells assumed stagnant growth and the growth seemed to stop at passage 7. The MTT assay revealed that for both normal and degenerated NP cells, the absorbance （.4） value at passages 4-7 was obviously decreased as compared with that at passage 1 （P〈0.05）. Cell cycle analysis showed that the proportion of normal NP cells at G1 phase was 65.4%-3.5%, significantly lower than that of degenerated NP cells at the same cell cycle phase With the value being 77.6%-4.8%. The degen- erated NP cells were predominantly arrested at Gt phase and failed to enter S phase. The expression of type II collagen and aggrecan was significantly decreased with passaging. It was concluded that normal NP cells possessed good viability and proliferative capacity by the third passage, and they could secrete large amounts of ECM within this period. The normal NP cells may serve as seed cells for IVD tissue engineering.

关键词： nucleus pulposus cells in vitro culture biological characteristics