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Insulin-like growth factor 2 as a candidate gene influencing growth and carcass traits and its bialleleic expression in chicken

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Science China(Life Sciences) 2005年第2期48卷 187-194页

作者： WANG Genyu1,YAN Bingxue1,DENG Xuemei2,LI Changlü2,HU Xiaoxiang1 & Li Ning1 1.State Key Laboratory for Agrobiotechnology,China Agricultural University,Beijing 100094,China 2.Department of Animal Genetics and Breeding,College of Animal Science and Technology,China Agricultural University,Beijing 100094,China State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China Department of Animal Genetics and Breeding College of Animal Science and Technology China Agricultural University Beijing 100094 China

We have identified DNA polymorphisms in the gene of insulin-like growth factor 2 by PCR-SSCP in a resource population, which was generated by Silky reciprocally crossing to Broilers. A C→G mutation was detected in the exon 2 (at position 71) by sequencing. This single nucleotide polymorphism (SNP) was found to be associated with production traits. Chicken with BB genotype showed more chest angle width but less 3 week body weight and glandular stom- ach weight than chicken with AA genotype (Pimprinting. Therefore, Silky chickens were selected for production of heterozygotes to confirm whether IGF2 locus was imprinting. Progeny from heterozygote × homozygote reciprocal cross was assayed for ex- pression after the genotype was determined. The transcription of IGF2 was detected by RT-PCR-SSCP. IGF2 gene was expressed bialleleically in 1-day-old neonatal liver and 90-day-old liver, kidney, heart, and muscle of both heterozygote AB and BA chickens. Therefore, IGF2 was not an imprinting.gene in chicken. The different genetic effects between the het- erozygote AB and BA remain to be elucidated.

关键词： chicken, IGF2, single nucleotide polymorphism, genome imprinting.

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