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Optimized Conditions for the Delivery of Small Membrane impermeable Compounds into Human Cells Using Hypotonic Shift

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CellBio 2012年第2期1卷 38-42页

作者： Alexandre S. Stephens Christopher J. Day Joe Tiralongo Institute for Glycomics Griffith University Gold Coast Campus Griffith University Queensland Australia

Cell-based assays represent a major end point of high throughput screening (HTS) but a key limitation of such assays is the potentially poor membrane permeability of test compounds. In this study, we optimized the conditions for the delivery of the membrane impermeable compound 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) into human cells using hypotonic shift;a method that can promote the uptake of molecules from the extracellular fluid into cell cytoplasm via endocytosis. We showed that uptake of HPTS by cells was a function of hypotonic buffer osmolarity and that delivery was highly efficient with almost 100% of cells displaying uptake. Delivery of HPTS was equally effective at 25°C and 37°C, with delivery of compound proportional to incubation time and concentration of HPTS within the loading medium. The experimental conditions identified in this study could be applied to HTS drug discovery studies providing an effective method of delivering small membrane impermeable compounds into cells.

关键词： Hypotonic Shift Cell-Based Assay Membrane impermeable Compound Delivery Osmolarity Endocytosis

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ASYMPTOTIC STABILITY OF THE RAREFACTION WAVE FOR THE NON-VISCOUS AND HEAT-CONDUCTIVE IDEAL GAS IN HALF SPACE

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Acta Mathematica Scientia 2019年第4期39卷 1195-1212页

作者： Meichen HOU School of Mathematical Sciences University of Chinese Academy of Sciences Institute of Applied Mathematics AMSS Beijing 100190 China Academy of Mathematics and Systems Science Academia Sinica

This article is concerned with the impermeable wall problem for an ideal polytropic model of non-viscous and heat-conductive gas in one-dimensional half space. It is shown that the 3-rarefaction wave is stable under some smallness conditions. The proof is given by an elementary energy method and the key point is to do the higher order derivative estimates with respect to t because of the less dissipativity of the system and the higher order derivative boundary terms.

关键词： Non-viscous impermeable problem rarefaction wave

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Maternal and burial environment determine the physical dormancy release in tropical Senna auriculata(Fabaceae) seeds

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Journal of Forestry Research 2019年第4期30卷 1343-1351页

作者： Ganesh K.Jaganathan Yingying Han Danping Song Panner Selvam Baolin Liu Institute of Biothermal Technology University of Shanghai for Science and TechnologyShanghai 200093People's Republic of China Agro Climate Research Centre Tamil Nadu Agricultural UniversityCoimbatoreTamil Nadu 641019India

In tropical ecosystems,species with an impermeable seed coat,*** dormancy(PY),are large in number and their seed coat is considered to be an adaptive trait for species persistence and ***,only little is known about their mechanisms for breaking *** objective of this study was to understand the importance of seed maturation site and burial location in determining the PY release of Senna *** collected seeds of *** from Vellore and Coimbatore,Tamil Nadu,India,germinated to 11±1.5%and19±2.5%respectively and remaining seeds did not imbibe water,thus had *** of seeds from both sites following hot-water treatment for 30 s and mechanical scarification increased significantly and seeds were able to germinate at a wide-range of temperatures(5–35℃)both in light and *** incubated at 15/60℃for 3 months,dormancy release for seeds collected from Coimbatore(72%)was greater than seeds matured in Vellore(53%).Following 1 year of burial at three different locations,seeds from Coimbatore germinated to higher percentage than Vellore seeds at all *** particular,the higher temperature sites released dormancy to greater extent than the lower temperature *** results suggest that summer temperatures(>60℃)prevailing in the tropics provide appropriate cues for breaking PY,but this could be greatly affected by the initial state of seeds and the burial environment.

关键词： Artificial burial Germination ecology impermeable seed coat Summer temperature

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Torsional Vibrations of Coated Hollow Poroelastic Spheres

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Open Journal of Acoustics 2017年第1期7卷 18-26页

作者： Syed Ahmed Shah Chinta Nageswaranath Modem Ramesh Mangipudi Venkata Ramanamurthy Department of Mathematics Deccan College of Engineering and Technology Hyderabad India Department of Mathematics Vardhaman College of Engineering Hyderabad India Department of Mathematics College of Technology Osmania University Hyderabad India Department of Mathematics Osmania University Hyderabad India

Torsional vibrations of coated hollow poroelastic spheres are studied employing Biot’s theory of wave propagation in poroelastic solid. The dilatations of solid and liquid media are zero, therefore the frequency equation of torsional vibrations is same both for a permeable and an impermeable surface. The coated poroelastic sphere consists of an inner hollow poroelastic sphere bounded by and bonded to a sphere made of distinct poroelastic material. The inner sphere is designated as core and outer sphere as casing. Core and casing are bonded at the curved surfaces. The inner and outer boundaries of the coated hollow poroelastic sphere are free from stress and at the interface of core and casing the displacement and stresses are continuous. It is assumed that the each material of coated sphere is homogeneous and isotropic. The frequency equation of torsional vibrations of a coated poroelastic hollow sphere is obtained when the material of the core vanishes. Also a coated poroelastic solid sphere is obtained as the limiting case of the frequency equation of coated hollow poroelastic sphere when the inner radius of core approaches to zero. Non-dimensional frequency as a function of ratio of thickness of core to that of inner radius of core is determined and analyzed. It is observed that the frequency and dispersion increase with the increase of the thickness of the coating.

关键词： Coated poroelastic Sphere Torsional Vibrations Permeable Surface impermeable Surface Frequency Equation Dissipation Mode of Vibration

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SCOUR AROUND GROINS

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International Journal of Sediment Research 2001年第1期16卷 60-68页

作者： Masoud GHODSIAN and S. M. Hoseini TEHRANI Assistant professor of Civil Engineering, Tarbiat Modarres University, P.O. Box 14115/143, Tehran- Iran. Post graduate student, I. A. University and Research Engineer, Water Research Center, Tehran- Iran. Tarbiat Modarres University P.O. Box 14115/143 Tehran- Iran I. A. University and Research Engineer Water Research Center Tehran- Iran

Based on the experimental data, the characteristics of maximum clear water scour hole around groins were studied by correlating to the approach velocity of flow, mean sediment size, depth of flow and ratio of length of permeable to impermeable part of groin. Empirical equations for prediction of scour hole characteristics were proposed based upon dimensional analysis and laboratory experiments.

关键词： Scour Groin Permeable impermeable Semipermeable

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