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Multiplexed imaging detection of live cell intracellular changes in early apoptosis with aggregation-induced emission fluorogens

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Science China Chemistry 2018年第8期61卷 892-897页

作者： Yabin Zhou Haixiang Liu Na Zhao Zhiming Wang Michael Z.Michael Ni Xie Ben Zhong Tang Youhong Tang Faculty of Biological Engineering Sichuan University of Science and Engineering Department of Chemistry the Hong Kong University of Science and Technology Centre for Nano Scale Science and Technology College of Science and Engineering Flinders University Department of Gastroenterology and hepatology Flinders Centre for Innovation in Cancer Flinders University AIEgen Biotech Co. Limited

Apoptosis is an important process for maintaining tissue homeostasis and eliminating abnormal cells in multicellular *** in apoptosis often leads to severe diseases such as cancers. Better understanding of its mechanisms and processes is therefore important. Accompanying molecular biology events of apoptosis is a series of cellular morphology changes: nucleus condensation, cell shrinkage and rounding, cell surface blebbing, dynamic blebbing, apoptotic membrane protrusions and nucleus fragmentations and finally, the formation and release of apoptotic bodies. It is difficult to detect cellular changes in the early phase of apoptosis due to the subtle changes at this phase. In the current study, we induced apoptosis in he la cells with H2 O2 and used nuclear dye Hoechst 33258, mitochondria, lysosome and cytoplasmic protein specific aggregation-induced emission fluorogens(AIEgens), TPE-Ph-In, 2 M-DABS and BSPOTPE to successfully perform live cell multiplexed imaging to investigate early apoptosis cellular events. We showed the gradual dissipation of mitochondria membrane potential until it is nondetectable by TPE-Ph-In. Increased mitophagy detected by TPE-Ph-In and 2 M-DABS, condensed nucleus detected by Hoechst33258, increased permeability and/or reduced integrity of nuclear membrane, and increased intracellular vesicles detected by2 M-DABS are some of the early events of apoptosis.

关键词： apoptosis multiplexed imaging he la aggregation-induced emission fluorogens(AIEgens)

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