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Danlou Tablet Improves Chronic Intermittent hypoxia-induced Dyslipidemia and Arteriosclerosis by HIF-1α-Angptl4 mRNA Signaling Pathway

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Chinese Journal of Integrative Medicine 2022年第6期28卷 509-517页

作者： TANG Jing-jing LI Guang-xi LIU Zhi-guo YI Rong YU Dong ZHANG Yue-bo ZHAO Shuang-qiao WANG Shi-han Department of Pneumology Guang’anmen HospitalChinese Academy of Chinese Medical SciencesBeijing100053China Department of Pulmonary and Critical Care Medicine Mayo ClinicRochesterMN55905USA Drug Clinical Trial Agency Guang’anmen HospitalChinese Academy of Chinese Medical SciencesBeijing100053China Department of Cardiovascular Medicine Mayo ClinicRochesterMN55905USA Department of Gynecology Guang’anmen HospitalChinese Academy of Chinese Medical SciencesBeijing100053China Department of Cardiovascular Medicine Guang’anmen HospitalChinese Academy of Chinese Medical SciencesBeijing100053China

Objective:To detect whether Danlou Tablet(DLT)regulates the hypoxia-induced factor(HIF)-1α-angiopoietin-like 4(Angptl4)mRNA signaling pathway and explore the role of DLT in treating chronic intermittent hypoxia(CIH)-induced dyslipidemia and ***:The mature adipocytes were obtained from3 T3-L1 cell culturation and allocated into 8 groups including control groups(Groups 1 and 5,0.1 mL of cell culture grade water);DLT groups(Groups 2 and 6,0.1 mL of 1,000μg/mL DLT submicron powder solution);dimethyloxalylglycine(DMOG)groups(Groups 3 and 7,DMOG and 0.1 mL of cell culture grade water);DMOG plus DLT groups(Groups 4 and 8,DMOG and 0.1 mL of 1,000μg/mL DLT submicron powder solution).Groups1-4 used mature adipocytes and groups 5-8 used HIF-1α-siRNA lentivirus-transfected mature *** 24-h treatment,real-time polymerase chain reaction and Western blot were employed to determine the mRNA and protein expression levels of HIF-1αand *** animal experiments,the CIH model in ApoE^(-/-)mice was *** mice were complete randomly divided into 4 groups including sham group,CIH model group[intermittent hypoxia and normal saline(2 mL/time)gavage once a day],Angptl4 Ab group[intermittent hypoxia and Angptl4 antibody(30 mg/kg)intraperitoneally injected every week],DLT group[intermittent hypoxia and DLT(250 mg/kg)once a day],4 mice in each *** 4-week treatment,enzyme linked immunosorbent assay was used to detect the levels of serum total cholesterol(TC)and triglyceride(TG).Hematoxylin-eosin and CD68 staining were used to observe the morphological properties of arterial ***:Angptl4 expression was dependent on HIF-1α,with a reduction in mRNA expression and no response in protein level to DMOG or DLT treatment in relation to siHIF-1α-transfected *** inhibited HIF-1αand Angptl4 mRNA expression(P<0.05 or P<0.01)and reduced HIF-1αand Angptl4 protein expressions with DMOG in mature adipocytes(all P<0.01),as the effect on

关键词： Danlou Tablet Chinese medicine hypoxia-induced factor angiopoietin-like 4 dyslipidemia arteriosclerosis

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