检索结果-南通市图书馆

Developmental patterns of serum leptin levels, leptin gene expression in adipose tissue and Ob-Rb gene expression in hypothalamus of Erhualian and Large White pigs

引用

Science China(Life Sciences) 2004年第2期47卷 190-196页

作者： ZHOU Jie’ 2, ZHAO Ruqian1, WEI Xihui1, XIA Dong1, XU Qingfu1 & CHEN Jie1 1. Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China 2. Anhui Agricultural University, Hefei 230036, China Key Laboratory of Animal Physiology and Biochemistry Ministry of Agriculture Nanjing Agricultural University Nanjing 210095 China Anhui Agricultural University Hefei 230036 China Anhui Agricultural University Hefei 230036 China Key Laboratory of Animal Physiology and Biochemistry Ministry of Agriculture Nanjing Agricultural University Nanjing 210095 China

The present study was aimed to investigate the developmental patterns of leptin mRNA expression in dorsal subcutaneous adipose tissue and Ob-Rb mRNA expression in hy[(-)-23.8( )]pothalamus in pigs of different breeds and sexes. [(Erhualian gilts and boars and Large White)0( )]boars were sampled at birth, 3, 20, 30, 45, 90, 120 and 180 days of age, respectively. Serum concentration of leptin was measured with RIA and single tube semi-quantitative RT-PCR was applied to determine the relative abundances of mRNA expression using 18S rRNA as an internal standard. The results showed that leptin mRNA expression in adipose tissue increased with age and displayed both sex and breed differences. In Erhualian pigs, females expressed higher leptin mRNA compared with males, and Erhualian boars showed higher abundance of leptin mRNA than Large White boars (P hypothalamus exhibited a distinctive pattern, decreased gradually after birth, and then in[(-)23.8( )][(creased till weaning. )0(After weaning, Ob)0(-)]Rb gene expression decreased gradually with age but rose gradually again from 120 to 180 days of age in Erhualian pigs. [(The expression of Ob)0(-)(Rb)( )]mRNA was higher in Large White pigs than that in Erhualian pigs (P< 0.01). The results suggest that the serum leptin level and leptin gene expression in adipose tissue highly correlate with [(adiposity.)0( )]

关键词： pigs, adipose tissue, hypothalamus, leptin, long form leptin recepter (Ob-Rb).

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：