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OBSERVATION ON REGIONAL DIFFERENCES OF SOME DEHYDROGENASES AND hydrolases IN RAT EPIDIDYMIS

中国组织化学与细胞化学杂志 1992年第1期1卷 118-125,149-150页

作者： Zhang Junhui, Wu Lijun Wu Jingmei Wu Mingzhang (Shanghai Zo. 2 Medical University)

The epididymal epithelia, by secretion, fluid reabsorption and transition, provide a favorable environment for sperm maturation. We observed, with histochemical method, the regional differences of four hydrolases and ... 详细信息

关键词： rat epididymis epithelia in different segments spermatozoa in cavity hydrolases dehydrogenases

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Classification of Reactions Catalyzed by Oxidoreductases and hydrolases Using Computational Methods

Classification of Reactions Catalyzed by Oxidoreductases and...

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第五届全国生物信息学与系统生物学学术大会暨国际生物信息学前沿研讨会高通量时代的生物信息学与系统生物学

作者： Aixia Yan Xiaoying Hu State Key Laboratory of Chemical Resource Engineering Department of Pharmaceutical EngineeringP.O.Box 53Beijing University of Chemical Technology

The Enzyme Commission(EC) classification system is widely used in chemistry and biology and is maintained by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology(NC-IUBMB).Enzymes are classified into six main classes;each main class is reclassified into several subclasses;a subclass is classified into *** this EC system,each enzyme has its unique EC number:EC a.b.c.d,where "a" refers to the six main classes of enzymes;"b" indicates the subclasses;"c" represents the subsubclasses;"d" is the serial number of the enzyme in its *** method of enzyme classification is based on several criteria,such as reaction types,substrates,transferred groups,and acceptor groups. In this work,several computational models for classification of reactions catalyzed by hydrolases and oxidoreductases were developed using three different computational methods. The overall breaking and making of bonds of reactions were *** physicochemical properties for the reacting bond in the substrate of each enzymatic reaction were calculated to describe the characteristics of each *** 311 reactions catalyzed by hydrolases (EC 3.b.c.d) and the 651 reactions catalyzed by oxidoreductases(EC l.b.c.d) were classified by Kohonen’s Self-Organizing Map(SOM),by a support vector machine(SVM),and by hierarchical clustering analysis(HCA).For the 311 reactions catalyzed by hydrolases,the classification accuracy of 95.8%by a SOM and 97.7%by an SVM was *** the 651 reactions catalyzed by oxidoreductases,the classification accuracy was 93.4%and 96.3%by a SOM and a SVM,*** similarities of reactions reflected by the physicochemical effects of reacting bonds were compared with the traditional Enzyme Commission(EC) classification system.

关键词： hydrolases Oxidoreductase Reaction classification Kohonen’s Self-Organizing Map(SOM) support vector machine(SVM)

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Distribution of Soil Enzyme Activities and Microbial Biomass Along a Latitudinal Gradient in Farmlands of Songliao Plain,Northeast China

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Pedosphere 2008年第4期18卷 431-440页

作者： LIU Xiu-Mei LI Qi LIANG Wen-Ju JIANG Yong Key Laboratory of Terrestrial Ecological Process Institute of Applied Ecology Chinese Academy of Sciences Shenyang 110016 （China） Graduate School of the Chinese Academy of Sciences Beijing 10004g （China）

Soil enzymes activities and microbial biomass have an important influence on nutrient cycling. The spatial distribution of soil enzymes activities and microbial biomass were examined along a latitudinal gradient in farmlands of Songliao Plain, Northeast China to assess the impact of climatic changes along the latitudinal transect on nutrient cycling in agroecosystems. Top soils （0-20 cm depth） were sampled in fields at 7 locations from north （Hallun） to south （Dashiqiao） in the end of October 2005 after maize harvest. The contents of total C, N, and P, C/N, available N, and available P increased with the latitude. The activities of invertase and acid phosphatase, microbial biomass （MB） C and N, and MBC/MBN were significantly correlated with latitude （P 〈 0.05, r^2 = 0.198, 0.635, 0.558, 0.211 and 0.317, respectively）, that is, increasing with the latitude. Significant positive correlations （P 〈 0.05） were observed between invertase activity and the total N and available P, and between acid phosphatase activity and the total C, C/N, available N, total P and available P. The urease, acid phosphatase, and dehydrogenase activities were significantly correlated with the soil pH and electrical conductivity （EC）（P 〈 0.05）. MBC and MBN were positively correlated with the total C, C/N, and available P （P 〈 0.05）. The MBC/MBN ratio was positively correlated with the total C, total N, C/N, and available N （P 〈 0.05）. The spatial distribution of soil enzyme activities and microbial biomass resulted from the changes in soil properties such as soil organic matter, soil pH, and EC, partially owing to variations in temperature and rainfall along the latitudinal gradient.

关键词： farmlands hydrolases latitudinal gradient microbial biomass soil organic matter

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New possibility for PET plastic recycling by a tailored hydrolytic enzyme

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Green Energy & Environment 2024年第2期9卷 163-165页

作者： Shijie Yu Qinghai Li Yanguo Zhang Hui Zhou Key Laboratory for Thermal Science and Power Engineering of Ministry of Education Beijing Key Laboratory of CO_(2)Utilization and Reduction TechnologyDepartment of Energy and Power EngineeringTsinghua UniversityBeijing 100084China

Plastic waste puts a huge burden on the ecosystem due to the current lack of mature recycling ***(ethylene terephthalate)(PET)is one of the most produced plastics in the *** decomposition holds the promise of recovering monomers from PET plastic,and the monomers can be used to regenerate new PET ***,there are still limitations in the activity and thermal stability of the existing PET *** recent study by Lu et *** a novel PET hydrolase via machine learning-aided *** obtained PET hydrolase showed excellent activity and thermal stability in the hydrolysis of PET and is capable of directly degrading large amounts of postconsumer PET *** approach provides an effective method for recycling PET waste and is expected to improve the current state of plastic pollution worldwide.

关键词： Plastic waste Poly(ethylene terephthalate) Hydrolysis Machine learning Enzymatic depolymerization hydrolases

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Arylsulfatase,β-galactosidase and lysozyme in gastric cancer cells and its relationship to invasion

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World Journal of Gastroenterology 1998年第1期4卷 57-59页

作者： YI YongFen and HUANG YouRong

IM To study the distribution of arylsulfatase, βgalactosidase and lysozyme in gastric cancer cells, and its relationship to differentiation and invasion of gastric cancer cells.METHODS Histochemical, immunohistochemical and ruthenium red (RR) electrocytochemical technique for three types of hydrolases and proteoglycans in pericancerous matrix in 33 cases of gastric cancer were observed under light and electron microscopy.RESULTS The expression intensities of arylsulfatase, βglactosidase and lysozyme in mucinous cell carcinomas were more intensive than those in welldifferentiated and poorlydifferentiated adenocarcinomas (Phydrolases into extracellular matrix, inducing degradation of pericancerous matrix and facilitating cancer cell invasion and metastasis..

关键词： tomach neoplasms hydrolases proteoglycans histochemistry neoplasm invasiveness arylsulfatase betagalactosidase muramidase

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Chemoenzymatic Synthesis of an Enantiomerically Enriched Bicyclic Carbocycle Using <i>Candida parapsilosis</i>ATCC 7330 Mediated Enantioselective Hydrolysis

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International Journal of Organic Chemistry 2015年第4期5卷 271-281页

作者： Thangavelu Saravanan Anju Chadha Tarur Konikkaledom Dinesh Namasivayam Palani Sengottuvelan Balasubramanian Laboratory of Bioorganic Chemistry Department of Biotechnology Indian Institute of Technology Madras Chennai India National Center for Catalysis Research Indian Institute of Technology Madras Chennai India Department of Inorganic Chemistry University of Madras Guindy Campus Chennai India ITC Life Sciences and Technology Centre ITC Limited Peenya Industrial Area Phase I Bangalore India

Enantiomerically enriched (R)-1-(2-bromocycloalkenyl)-3-buten-1-ol and its derivatives were obtained via enantioselective hydrolysis [resolution] with good enantioselectivities (E = 31 to >500) using Candida parapsilosis ATCC 7330. The various reaction parameters were optimized for enantioselective hydrolysis to achieve high enantiomeric excess (ee) and conversions. Among the substrates tested, (RS)-1-(2-bromocyclohex-1-en-1-yl) but-3-yn-1-yl acetate was hydrolysed by the biocatalyst in 12 h to the corresponding (R)-alcohol in 49% conversion and >99 ee. The optically pure allylic alcohol thus obtained was used as a chiral starting material for the synthesis of an enantiomerically enriched bicyclic alcohol effectively establishing achemoenzymatic route.

关键词： Chemoenzymatic Synthesis Enantioselective Hydrolysis Candida parapsilosis ATCC 7330 hydrolases Bicyclic Carbocycle

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