检索结果-南通市图书馆

Hyaluronic acid-based hydrogels with tobacco mosaic virus containing cell adhesive peptide induce bone repair in normal and osteoporotic rats

在线全文

cnki会议

学校读者我要写书评

暂无评论

Biomaterials Translational 2020年第1期1卷 89-98页

作者： Jishan Yuan Panita Maturavongsadit Zhihui Zhou Bin Lv Yuan Lin Jia Yang Jittima Amie Luckanagul Department of Orthopaedic Surgery the Affiliated First People’s Hospital to Jiangsu UniversityZhenjiangJiangsu ProvinceChina Department of Chemistry and Biochemistry University of South CarolinaColumbiaSCUSA Joint Department of Biomedical Engineering North Carolina State University and The University of North Carolina at Chapel HillChapel HillNCUSA The State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchunJilin ProvinceChina Department of Pharmaceutics and Industrial Pharmacy Faculty of Pharmaceutical SciencesChulalongkorn UniversityBangkokThailand Research Unit for Plant-produced Pharmaceuticals Chulalongkorn UniversityBangkokThailand.

Tobacco mosaic virus(TMV)has been studied as a multi-functional agent for bone tissue engineering.An osteo-inductive effect of wild-type TMV has been reported,as it can significantly enhance the bone differentiation potential of bone marrow stromal cells both on a two-dimensional substrate and in a three-dimensional(3D)hydrogel system.A TMV mutant(TMV-RGD1)was created which featured the adhesion peptide arginyl-glycyl-aspartic acid(RGD),the most common peptide motif responsible for cell adhesion to the extracellular matrix,on the surface of the virus particle to enhance the bio-functionality of the scaffold material.We hypothesised that the incorporation of either wild-type TMV or TMV-RGD1 in the 3D hydrogel scaffold would induce bone healing in critical size defects of the cranial segmental bone.We have previously tested the virus-functionalised scaffolds,in vitro,with a hyaluronic acid-based system as an in-situ hydrogel platform for 3D cell encapsulation,culture,and differentiation.The results of these experiments suggested the potential of the virus-functionalised hydrogel to promote in vitro stem cell differentiation.The hydrogel-forming system we employed was shown to be safe and biocompatible in vivo.Here,we further explored the physiological responses regarding bone regeneration of a calvarial defect in both normal and osteoporotic ovariectomized rat models.Our results,based on histological analysis in both animal models,suggested that both wild-type TMV and TMV-RGD1 functionalised hydrogels could accelerate bone regeneration,without systemic toxicity,evaluated by blood counts.New bone formation was intensified by the incorporation of the RGD-mutant viral particles.This finding increased the potential for use of the rodshaped plant virus as a platform for the addition of powerful biofunctionality for tissue engineering applications.This study was approved by the Ethics Committee on Animal Use of the Zhenjiang Affiliated First People’s Hospital affiliated to Jiangsu University.

关键词： bone regeneration calvarial defect hydrogel osteoporosis tobacco mosaic virus

高性能杂化水凝胶的制备及其性能研究（英文）

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

高性能杂化水凝胶的制备及其性能研究（英文）

中国化学会2017全国高分子学术论文报告会

作者：李江波刘汉超黄光速郑静四川大学高分子科学与工程学院高分子材料工程国家重点实验室

Introducing both chemical and physical cross-links into a polymer network has emerged as a prevalent strategy to toughen hydrogels. However, most of these hybrid hydrogels are prepared on a multi-step routes with more than one polymer or cross-linker, which is sophisticated and time consuming. Here, we report on a facile fabrication by in-situ polymerizing acrylamide with a single multifunctional cross-linker. Compressive and tensile tests show the resultant hydrogels exhibit superior mechanical toughness. They can be stretched up to 1900% with a maximal fracture stress of nearly 500 kP a and be compressed to 90%. More impressively, the hydrogels can be fully recovered under compression. Rheological measurements suggest the co-existence of physical and chemical cross-links. Finally, an effective energy dissipation mechanism is observed and discussed. We conclude that the reversible physical cross-links originated from entanglement provide a mechanism of energy dissipation while the chemical cross-links ensure the structural integrity, which could be responsible for the improved mechanical properties.

关键词： hydrogel mechanical toughness multifunctional cross-linker entanglement

hydrogels from Chitosan and a Novel Copolymer Poly(N-Vinyl-2-Pyrrolidone-Co-Acrolein)

在线全文

cnki会议

学校读者我要写书评

暂无评论

Materials Sciences and Applications 2011年第8期2卷 1058-1069页

作者： Janaina Aline Galvao Barros Antonio Jedson Caldeira Brant Luiz Henrique Catalani Institute of Chemistry University of Sao PauloSao PauloBrazil

PVP, a synthetic polymer and chitosan, a natural polymer are biocompatible and have presented a great variety of interesting properties for cosmetic, biomedical, pharmaceutical and biotechnological applications, Many alternatives to improve the polymer propeties has been made as bleeding polymers, for example. In this work, two different techniques of hydrogel attainment were used: one from mixtures of acid aqueous cosolutions of chitosan and PVP, whose resultant films and solutions were irradiated, afterwards, by ultraviolet radiation (λ = 254 nm), another one from the reaction of poly (N-vinyl-2-pyrrolidone-co-acrolein)-a novel copolymer synthesized in our biomaterials laboratory-and chitosan. In the first one alternatives it was possible produce hydrogels directly from mixtures of aqueous acidic co-solutions of both polymers. In the second one, the attainment of hydrogels from Schiff base has proved to be an effective methodology for the production of hydrogels, showing good values of gel content and swelling.

关键词： Chitosan hydrogel Copolymer Crosslinking Schiff Base Poly(Vinyl-Pyrrolidone-Co-Acrolein)

Simplifying Molecular Complexity for the Synthesis of Polymer Materials

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Simplifying Molecular Complexity for the Synthesis of Polyme...

中国化学会2017全国高分子学术论文报告会

作者： Patrick Théato University of Hamburg Institute for Technical and Macromolecular Chemistry

The increasing complexity on the molecular level demands for highly advanced specialists possessing the skillset to synthesize such chemical structures. This clearly limits or slows down the advancement to new scientific areas. Hence, we have addressed this challenge over the years by developing simple synthetic routes, while maintaining a molecular complexity, thereby providing the synthetic tools to prepare highly functional polymer.

关键词： post-polymerization modification smart polymers hydrogel

A Novel Design Strategy for Temperature-Responsive IPN hydrogels Based on a Copolymer of Acrylamide and N-(1,1-Dimethyl-3-Oxobutyl)-Acrylamide

在线全文

cnki会议

学校读者我要写书评

暂无评论

Advances in Chemical Engineering and Science 2018年第4期8卷 255-270页

作者： Yu Wang Huifang Xia Jun Zhao Xiuqin Cai Shengke Chen Bengxiu Li School of Chemistry and Materials Sciences Guizhou Education University Guiyang China Anshun Engineering Research Centre of Concrete Admixture Anshun China School of Chemistry and Materials Science Weinan Normal University Weinan China GuizhouTiejinahengfa New Material Technology Co. Ltd. Anshun China

A methology is described for the synthesis of novel temperature-responsive interpenetrating polymer network (IPN) hydrogels with poly(2-acrylamido- 2-methylpropane sulfonic acid) (PAMPS) as a tightly crosslinked 1st network, temperature-responsive poly(acrylamide-co-N-(1,1-dimethyl-3-oxobutyl)- acrylamide) (P(AM-co-DAAM)) with low cost as a lossely crosslinked 2nd network. The structure and morphology of the IPN hydrogels were characterized by FTIR, TGA and SEM, and the results indicated that PAMPS network introduced P(AM-co-DAAM) hydrogels have large, thermally stable and interconnected porous network. The properties of the IPN hydrogels, which include: swelling capacity, equilibrium swelling/deswelling ratio, temperature- responsive behavior, and the dwelling kinetics as specific temperature, were investigated carefully. Results showed that the obtained IPN hydrogels displayed a controllable equilibrium swelling/deswelling behavior and possessed remarkable thermosensitivity. In addition, the results also indicate that the incorporation of the hydrophobic groups DAAM has a big effect on the LCST of the IPN hydrogels. Consequently, these novel temperature-responsive IPN hydrogels with low cost and slow-releasing performance would be promising for potential applications, such as environmental catalysis, water treatment, and agriculture.

关键词： Temperature-Responsive hydrogel Interpenetrating Polymer Network Equi-librium Swelling Ratio Slow-Releasing Performance

Synthesis and Characterization of Poly (hydroxylethyl, octanediamine)-DL-aspartamide: a New Tissue Engineering Scaffolds

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2006年第2期17卷 191-194页

作者： Gu Ping TANG Xiao Xiao SONG Da LI Department of Chemistry Zhejiang University Hangzhou 310028 Department of Medical Zhejiang University Hangzhou 310006

Poly （hydroxyethyl, octanediamine）-DL-aspartamide （PHOA） hydrogels were prepared by adding mixture of 1,8-octanediamine and ethanolamine to PA in DMF at room temperature. The properties of the hydrogels were inves... 详细信息

关键词： Octanediamine aspartamide hydrogel cell adhesion.

Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Transplantation of RADA16-BDNF peptide scaffold with human u...

中国神经科学学会第十二届全国学术会议

作者： Wei Shi Chuan-Jun Huang Xi-De Xu Yi-Nan Chen Qian-Qian Liu Jian Chen Department of Neurosurgery Affiliated Hospital of Nantong University

Objective Due to the poor self-regeneration of brain tissue, stem cell transplantation therapy is purported to enable the replacement of lost neurons after traumatic brain injury（TBI）. The main challenge of brain regeneration is whether the transplanted cells can survive and carry out neuronal functions in the lesion area. Methods We co-cultured human umbilical cord mesenchymal stem cells（h UC-MSCs） directly with the activated astrocytes and seeded in a biomaterial scaffold for the cell transplantation. We further specifically linked a functional peptide derived from BDNF, to the C-terminal of RADARADARADARADA（RADA16） to structure a functional self-assembling peptide hydrogel scaffold, RADA16-BDNF（R-B-SPH scaffold） for the better transplantation of the double cell unit（h UC-MSCs/astrocyte）. Also, the novel scaffold was used as cell-carrier for transplantation double cell unit for treating traumatic brain injury. Results（1） We found that co-culturing human umbilical cord mesenchymal stem cells（h UC-MSCs） directly with the activated astrocytes benefited to the proliferation and neuron differentiation of h UC-MSCs in vitro.（2）We first designed the RADA16-BDNF peptide scaffold such that it could not only flatten the injured brain cavity and reduce the surrounding reactive gliosis but could also provide neurotrophic factors.（3）We first specifically transferred CXCR4 to h UC-MSCs to improve the migratory ability of the transplanted h UC-MSCs CXCR4.The presence of h UC-MSCs/activated astrocytes in the co-culturing unit also promoted better neuronal differentiation of the NSCs.（4）Both h UC-MSCs CXCR4 and activated astrocytes transplanted in the R-B-SPH scaffolds proliferate in the transplantation area and the presence of activated astrocytes increased the proliferation of h UCMSCs CXCR4 in vivo.（5）SDF-1 induces the migration of transplanted h UC-MSCs and showed this process can be enhanced by transferring CXCR4 to h UC-MSCs.（6） We found that the moderate-sized lesion cavity, but not the large cavity caused by TBI was rep

关键词： Human umbilical cord mesenchymal stem cells BDNF hydrogel SDF1/CXCR4 axis Neural differentiation tissues engineering

Synthesis and Properties of IPN hydrogels Based on Konjac Glucomannan and Poly(acrylic acid)

在线全文

cnki会议

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2006年第10期17卷 1377-1380页

作者： Bing LIU Zhi Lan LIU Ren Xi ZHUO Key Laboratory of Biomedical Polymers of Ministry of Education Department of Chemistry Wuhan University Wuhan 430072

Novel interpenetrating polymer network （IPN） hydrogels based on konjac glucomannan （KGM） and poly（acrylic acid）（PAA） were prepared by polymerization and cross-linking of acrylic acid （AA） in the pre-fabricated KGM gel. The IPN gel was analyzed by FF-IR. The studies on the equilibrium swelling ratio of IPN hydrogels revealed their sensitive response to environmental pH value. The results of in vitro degradation showed that the IPN hydrogels retain the enzymatic degradation character of KGM.

关键词： hydrogel interpenetrating polymer network pH-sensitivity konjac glucomannan poly（acrylic acid）.

Click Synthesis of Thermosensitive hydrogels by Catalyst-free 1,3-Dipolar Cycloaddition Reaction

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Click Synthesis of Thermosensitive Hydrogels by Catalyst-fre...

河南省化学会2016年学术年会

作者： Hongzheng Zhu Huijuan Chu Hongliang Wei Jing Zhu Gang Wang School of Chemistry and Chemical Engineering Henan University of Technology

The properties and structures of hydrogels are closely linked to the method of preparing hydrogels,therefore, it has been considered to be very important to develop new methods of preparing hydrogels.Among them, preparing hydrogels by alkyne-azide 1, 3-dipolar cycloaddition click reaction is an important method which has appeared recently. The reaction has some advantages such as simple, mild conditions, no toxic and volatile solvent, etc. However, the cytotoxicity of residual Cu catalyst brings a potential limitation of the reaction when it is used to synthesize and/or functionalize materials for biomedical applications. Herein, replacing alkyne by maleimide group, catalyst-free 1, 3-dipolar cycloaddition click reaction between azide and maleimide modified polymers was used to prepare thermosensitive hydrogels. First, three copolymers(denoted respectively as PT137, PT155, PT173) of2-hydroxyethyl methacrylate(HEMA), N-isopropyl acrylamide(NIPAM) and N, N-dimethylacrylamide(DMA) were prepared by controlling the feeding molar ratios(HEMA: NIPAM: DMA) to 1:3:7, 1:5:5,1:7:3, respectively. The polymeric dipolarophiles were prepared by introducing N-maleoyl alanine to the PT137, PT155, PT173, The polymeric dipolar was prepared by modifying the as-prepared PT137,PT155, PT173 with azidoacetic acid. Afterward, the above prepared polymeric dipolarophiles and polymeric dipolar were dissolved in water, and the thermosensitive hydrogels of different molar ratios were prepared through catalyst-free 1, 3-dipolar cycloaddition reaction. The products were characterized by GPC, FTIR, 1HNMR, SEM, TG and other instruments. The swelling behavior of the thermosensitive hydrogels was studied, and the results showed that the hydrogels had good abilities of swelling and thermosensitivily.

关键词： hydrogel 1,3-dipolar cycloaddition click reaction thermosensitivity