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3D printing of silk fibroin-based hybrid scaffold treated with platelet rich plasma for bone tissue engineering

Bioactive Materials 2019年第1期4卷 256-260页

作者： Liang Wei Shaohua Wu Mitchell Kuss Xiping Jiang Runjun Sun Reid Patrick Xiaohong Qin Duan Bin School of Textile Science and Engineering Xi'an Polytechnic UniversityXi'an710048PR China Mary&Dick Holland Regenerative Medicine Program Division of CardiologyDepartment of Internal MedicineUniversity of Nebraska Medical CenterOmahaNE68198USA Key Laboratory of Textile Science&Technology Ministry of EducationCollege of TextilesDonghua UniversityShanghai201620PR China College of Textiles&Clothing Qingdao UniversityQingdao266071PR China Department of Pathology&Microbiology College of MedicineUniversity of Nebraska Medical CenterOmahaNE68198USA Department of Surgery College of MedicineUniversity of Nebraska Medical CenterOmahaNE68198USA Department of Mechanical and Materials Engineering University of Nebraska-LincolnLincolnNE68516USA

3D printing/bioprinting are promising techniques to fabricate scaffolds with well controlled and patient-specific structures and architectures for bone tissue *** this study,we developed a composite bioink consisting of silk fibroin(SF),gelatin(GEL),hyaluronic acid(HA),and tricalcium phosphate(TCP)and 3D bioprinted the silk fibroin-based hybrid *** 3D bioprinted scaffolds with dual crosslinking were further treated with human platelet-rich plasma(PRP)to generate PRP coated ***/Dead and MTT assays demonstrated that PRP treatment could obviously promote the cell growth and proliferation of human adipose derived mesenchymal stem cells(HADMSC).In addition,the treatment of PRP did not significantly affect alkaline phosphatase(ALP)activity and expression,but significantly upregulated the gene expression levels of late osteogenic *** study demonstrated that the 3D printing of silk fibroin-based hybrid scaffolds,in combination with PRP post-treatment,might be a more efficient strategy to promote osteogenic differentiation of adult stem cells and has significant potential to be used for bone tissue engineering.

关键词： 3D bioprinting hybrid scaffold Coating Growth factor cocktail Tissue engineering

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Fabrication of a Novel hybrid scaffold for Tissue Engineered Heart Valve

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2009年第5期29卷 599-603页

作者：洪昊董念国史嘉玮陈思郭超胡平齐宏旭 Department of Cardiovascular Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Chemical Engineering Institute of Polymer Science and TechnologyTsinghua University

The aim of this study was to fabricate biomatrix/polymer hybrid scaffolds using an electrospinning technique. Then tissue engineered heart valves were engineered by seeding mesenchymal stromal cells （MSCs） onto the scaffolds. The effects of the hybrid scaffolds on the proliferation of seed cells, formation of extracellular matrix and mechanical properties of tissue engineered heart valves were investigated. MSCs were obtained from rats. Porcine aortic heart valves were decellularized, coated with poly（3-hydroxybutyrate-co-4-hydroxybutyrate） using an electrospinning technique, and reseeded and cultured over a time period of 14 days. In control group, the decellularized valve scaffolds were reseeded and cultured over an equivalent time period. Specimens of each group were examined histologically （hematoxylin-eosin [HE] staining, immunohistostaining, and scanning electron microscopy）, biochemically （DNA and 4-hydroxyproline） and mechanically. The results showed that recellularization was comparable to the specimens of hybrid scaffolds and controls. The specimens of hybrid scaffolds and controls revealed comparable amounts of cell mass and 4-hydroxyproline （P〉0.05）. However, the specimens of hybrid scaffolds showed a significant increase in mechanical strength, compared to the controls （P〈0.05）. This study demonstrated the superiority of the hybrid scaffolds to increase the mechanical strength of tissue engineered heart valves. And compared to the decellularized valve scaffolds, the hybrid scaffolds showed similar effects on the proliferation of MSCs and formation of extracellular matrix. It was believed that the hybrid scaffolds could be used for the construction of tissue engineered heart valves.

关键词： tissue engineered heart valve hybrid scaffold electrospinning mesenchymal stem cells

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