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Effects of Terminalia arjuna bark extract on apoptosis of human hepatoma cell line HepG2

World Journal of Gastroenterology 2006年第7期12卷 1018-1024页

作者： Sarveswaran Sivalokanathan Marati Radhakrishnan Vijayababu Maruthaiveeran Periyasamy Balasubramanian Department of Pharmacology and Environmental Toxicology Dr.ALM Post Graduate Institute of Basic Medical SciencesUniversity of MadrasTaramani CampusChennai-600 113Tamil NaduIndia Department of Endocrinology Dr.ALM Post Graduate Institute of Basic Medical SciencesUniversity of MadrasTaramani CampusChennai-600 113Tamil NaduIndia

AIM：To investigate the effects of Terminalia arjuna （T. arjuna） extract on human hepatoma cell line （HepG2） and its possible role in induction of apoptosis.METHODS： human hepatoma cells were treated with different concentrations of ethanolic extract of T. arjuna and its cytotoxicity effect was measured by trypan blue exclusion method and lactate dehydrogenase leakage assay. Apoptosis was analyzed by light and fluorescence microscopic methods, and DNA fragmentation. The mechanism of apoptosis was studied with expression of p53 and caspase-3 proteins. Glutathione （GSH） content was also measured in HepG2 cells after T. arjuna treatment.RESULTS： T. arjuna inhibited the proliferation of HepG2 cells in a concentration-dependent manner. Apoptotic morphology was observed in HepG2 cells treated with T. arjuna at the concentrations of 60 and 100 mg/L. DNA fragmentation, accumulation of p53 and cleavage of procaspase-3 protein were observed in HepG2 cells after the treatment with T. arjuna. The depletion of GSH was observed in HepG2 cells treated with T. arjuna.CONCLUSION： T. arjuna induced cytotoxicity in HepG2 cells in vitro. Apoptosis of HepG2 cells may be due to the DNA damage and expression of apoptotic proteins. Depletion of GSH may be involved in the induction of apoptosis of HepG2 cells.

关键词： human hepatoma cell line Terrninalia arjuna Apoptosis p53 Caspase-3 GSH

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Inhibitory effect of parvovirus H-1 on the formation of colonies of human hepatoma cell line in vitro and its tumors in nude mice

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cell Research 1994年第1期4卷 47-56页

作者： YAN SHANGJUN CHENGWU MA XIANHUA CHEN SHANHONG WAN ZUYU LUO(Physiology and Biophysics Department, Fudan University,Shanghai 200433, China) Physiology and Biophysics Department Fudan University Shanghai China

The inhibitory effect of parvovirus H-1 on the colonyforming ability in vitro of QGY-7703, a cultured human hepatoma cell line, and on the formation and growth of its tumors in nude mice was studied. With higher multiplicity of infection (MOI) of H-1 given, survival of the QGY-7703 cells was found to be decreased. H-1 DNA amplification level at 30 h postinfection(p.i.) was detected to be 7.4 times higher than that at 2 h by dispersed cells assay, while the cells were delayed to enter into S *** were formed in the indicator cells (new-born human kidney cell line, NBK) by progeny H-1 virus particles released from the infected QGY-7703 cells by infectious cell center assay. The formation of tumors in nude mice by QGY-7703 cells which were injected s c at 2 h postinfection was observed to be prevented in 2 groups with given MOI 25 and 50. The tumor growth of MOI 10 group occurred at a lower exponential rate than that of control,after a 20 d latent period. It was evident that parvovirus H-1 exhibited a direct inhibitory effect on the formation and growth of human hepatoma cells in vivo as well as in vitro.

关键词： Parvovirus H-1 human hepatoma cell line colony formation nude mice inhibitory effect

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Melatonin and Doxorubicin synergistically induce cell apoptosis in human hepatoma cell lines

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World Journal of Gastroenterology 2010年第12期16卷 1473-1481页

作者： Fan, Lu-Lu Sun, Guo-Ping Wei, Wei Wang, Zhang-Gui Ge, Lei Fu, Wei-Zheng Wang, Hua Anhui Med Univ Affiliated Hosp 1 Dept Oncol Hefei 230022 Anhui Peoples R China Anhui Med Univ Inst Clin Pharmacol Key Lab Res & Dev Chinese Med Key Lab Antiinflammatory & Immunol Pharmacol Hefei 230022 Anhui Peoples R China Anhui Med Univ Affiliated Prov Hosp Dept Oncol Hefei 230032 Peoples R China

AIM:To investigate whether Melatonin has synergistic effects with Doxorubicin in the growth-inhibition and apoptosis-induction of human hepatoma cell lines HepG2 and ***:The synergism of Melatonin and Doxorubicin inhibited the cell growth and induced cell apoptosis in human hepatoma cell lines HepG2 and *** viability was analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide(MTT)*** apoptosis was evaluated using TUNEL method and flow ***-related protein Bax,Bcl-2 and caspase-3 expressions were measured by immunohistochemical ***:Treatment with Melatonin(10 -8 -10 -5 mol/L) alone had a dose-related inhibitory effect on cell proliferation but no cytotoxic effect on hepatoma cell lines HepG2 and ***,when combined with Doxorubicin,Melatonin significantly increased the effects of cell growth inhibition and cell ***,TUNEL staining and flow cytometry revealed that cooperative apoptosis induction was associated with decreased expression of Bcl-2 as well as increased expression of Bax and ***:The synergism of Melatonin and Doxorubicin inhibits hepatoma cell growth and induces cell apoptosis.

关键词： Melatonin Doxorubicin human hepatoma cell line Apoptosis

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Synergistic effect of combining paeonol and cisplatin on apoptotic induction of human hepatoma cell lines

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Acta Pharmacologica Sinica 2007年第6期28卷 869-878页

作者： Shu-ping XU~2,Guo-ping SUN~(4,5),Yu-xian SHEN~2,Wan-ren PENG~4,Hua WANG~3,Wei WEI~2~2 Institute of Clinical Pharmacology,Anhui Medical University,Key Laboratory of Anti-inflammatory and Immunological Pharmacology,Key Laboratory of Research and Development of Chinese Medicine,Hefei 230032,China ~3 Department of Oncology,Provincial Hospital ofAnhui,Hefei 230001,China ~4 Department of Oncology,The First Affiliated Hospital of Anhui Medical University,Hefei 230022,China Institute of Clinical Pharmacology Anhui Medical University Key Laboratory of Anti-inflammatory and Immunological Pharmacology Key Laboratory of Research and Development of Chinese Medicine Hefei China Department of Oncology The First Affiliated Hospital of Anhui Medical University Hefei China Department of Oncology Provincial Hospital of Anhui Hefei China

Aim:To investigate whether paeonol （Pae） has synergistic effects with cisplatin（CDDP） on the growth-inhibition and apoptosis-induction of human hepatomacell lines HepG2and ***:The cytotoxic effect of drugswas measured by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl-tetrazolium *** coefficient of drug interaction was used to analyze the nature of *** changes were observed by acridine orange fluo-rescence *** cycle and the apoptosis rate were detected by ***-2 and Bax expression were assayed by ***:Pae or CDDP had antiproliferative effect on the 2 cell linesin a dose-dependent manner,with different sensitivities to ***,a synergistic inhibitory effect on the viability of the 2 cell lineswas observed after treatment with a combination of Pae （15.63,31.25,and 62.5mg/L） with various concentrations of *** study showed typical mor-phological changes of apoptosis if the cells were exposed to the two agents for24 *** apoptotic rate of the cells with combination treatment was signifi-cantly higher than that of cells treated with Pae or CDDP *** expressionof Bcl-2 decreased and that of Bax increased in the treated groups,especially inthe combination group,with the ratio of Bcl-2/Bax decreasing ***,a combination of Pae with CDDP resulted in a stronger S phasearrest,compared with Pae or CDDP ***:Pae,in combinationwith CDDP,had a significantly synergistic growth-inhibitory and apoptosis-in-ducing effect on the 2 human hepatoma cell lines,which may be useful inhepatoma treatment.

关键词： paeonol cisplatin drug interaction human hepatoma cell line apoptosis cell cycle

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Effect of Calmodulin and Voltage-dependent Ca^(2+) Channel on the Proliferation of Heptoma cells Induced by Epidermal Growth Factor

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2003年第1期23卷 26-28页

作者：吴斌文王家 袁顺玉崔武任 InstituteofLiverDisease TongjiHospitalHuazhongUniversityofScienceandTechnology DepartmentofNuclearMedicine TongjiMedicalCollegeHuazhongUniversityofScienceandTechnology

The effect of thyrosine kinase, calmodulin and voltage-dependent Ca 2+ channel on the proliferation of hepatoma cells induced by EGF was studied. hepatoma cell line SMMC7721 was cultured in RPMI1640 serum-free medium. DNA synthesis rate of hepatoma cells was measured by 3H-TdR incorporation. 10 -9 mol/L EGF could significantly stimulate the proliferation of hepatoma cells (Phepatoma cells (P> 0.05), but it had very significantly inhibitory effect on the proliferation of hepatoma cells induced by EGF (Phepatoma cells induced by EGF (P>0.05). It had no effect on the basic phase of cultured hepatoma cells (P>0.05). It is suggested that tyrosine kinase and Ca 2+-calmodulin-dependent pathway may play a critical role on the proliferation of heptoma cells induced by EGF, and voltage-dependent Ca 2+ channel is independent of the effect of EGF.

关键词： epidermal growth factor human hepatoma cell line Ca 2+-calmodulin-dependent pathway tyrosine kinase voltage-dependent Ca 2+ channel

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