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Studies on mechanism of cis9,trans11-CLA and trans10,cis12-CLA inducing apoptosis of human breast cancer cell line mcf-7

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The Chinese-German Journal of Clinical Oncology 2010年第10期9卷 583-589页

作者： Xianzi Wan Xianlin Yuan Xiangling Yang Yichen Li Ling Zhong Laboratory of Microbiology and Biochemical Pharmacy School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou 510006 China

Objective： The aim of the study was to explore the activities of cis9, trans11-CLA （C9, t11-CLA） and transl0, cis12-CLA （t10, c12-CLA） inhibiting tumor, and investigate their relationships with PPARy and apoptotic proteins, and mechanism of anti-cancer. Methods： The inhibitory rate, cell growth curve and apoptotic morphological observation of mcf-7 cells were obtained by MTT assay, trypan blue staining and Hoechst33342 fluorescence staining. The apoptotic rate and cell cycle were detected with flow cytometry. Transcriptional level of genes was detected with RT-PCR semi-quantitative method, and Western blot was performed to detect proteins levels. Results： The two CLA isomers could reduce cell proliferation （P 〈 0.05）, increase apoptotic rate （P 〈 0.05）, and increase obviously the transcriptional and protein levels of PPARy （P 〈 0.01）. The synchronism and correlation between the effects of CLA to PPARy and apoptotic proteins Bax, Bcl-2, Caspase 3 changes were found with the dose- and time-dependent manners. There was cooperative relation between the levels of PPARy and the rates of Bax/Bcl-2, Caspase 3 （small fragment） by experiments of PPARy inhibitor GW9662 and ligand Rosiglitazone. Conclusion： The apoptotic pathway of PPARy-Bcl-2-Caspase 3 signaling was found. The C9, t11-CLA and tl0, c12-CLA could inhibit mcf-7 cell proliferation and promote apoptosis via activating PPARy-Bcl-2-Caspase 3 pathway. CLA may be a kind of activator of PPARv.

关键词： conjugated linoleic acid (CLA) isomer peroxisome proliferators activated receptor y (PPARγ) apoptosis human breast cancer cell line mcf-7

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A comparison study of the cytotoxicity of salinomycin and salinomycin sodium toward human breast cancer stem cells as well as breast cancer cells

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Journal of Chinese Pharmaceutical Sciences 2011年第4期20卷 368-375页

作者：张杨王学清王坚成张烜张强北京大学医学部天然药物及仿生药物国家重点实验室药学院药剂学系北京100191

Salinomycin（SAL）,a polyether antibiotic isolated from Streptomyces albus,is widely used as an anticoccidial drug in poultry and other livestock and is furthermore fed to ruminescent animals to improve nutrient absorption and feed *** has recently been shown to act as a specific inhibitor of cancer stem *** present,the price of salinomycin sodium（SAL-Na） is 10 fold lower than that of salinomycin,however,there is no report about the comparison of the inhibitory effects of SAL and SAL-Na on cancer stem cells as well as cancer *** the present study,side population cells（SP cells）and non-SP cells （NSP cells）sorted from human breast cancer cell line mcf-7 were chosen as the models of cancer stem cells and cancer cells, *** assay was performed to compare the cytotoxicity of SAL and *** of all,SP cells were sorted from mcf-7 cells via FACSDiva flow ***,the sorted SP cells were identified with the surface makers（CD44~＋/CD24^-） of breast cancer stem ***,the inhibitory effects of SAL and SAL-Na were evaluated on the sorted SP cells and NSP *** showed that,as compared to breast cancer cells,the inhibitory effect of free SAL or free SAL-Na was more potent in breast cancer stem ***,the inhibitory effects of free SAL and free SAL-Na had no significant difference for the SP cells as well as the NSP cells when they were in the same ***,it suggested that salinomycin sodium should be considered as a potential candidate to take the place of salinomycin in cancer stem cells research,due to their similar inhibitory effects on cancer stem cells.

关键词： Salinomycin Salinomycin sodium cancer stem cells human breast cancer cell line mcf-7

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