检索结果-南通市图书馆

Preparation of tanshinone Ⅱ_(A) self-soluble microneedles and its inhibition on proliferation of human skin fibroblasts

引用

Chinese Herbal Medicines 2023年第2期15卷 251-262页

作者： Yanshan Zhan Xiaoqi Xu Xi Luo Ruiping Liu Yujian Lin Ping Zhao Jun Shi School of Chinese Material Medica Guangdong Pharmaceutical University of ChinaGuangzhou 510006China Department of Chemistry and Chemical Engineering Guangdong Pharmaceutical University of ChinaZhongshan 528458China Engineering&Technology Research of Topical Precise Drug Delivery System Guangzhou Higher Education Mega CenterGuangzhou 510006China

Objective:Hypertrophic scars(HS)are a variety of skin tissue fibrosis disease that occurs in human skin,the effective therapeutic method of which is still inaccessible up to *** a bioactive constituent of a well-known medical plant,Salvia miltiorrhiza(Danshen in Chinese),tanshinone Ⅱ_(A)(TSA)is reported to inhibit cell proliferation in ***,the aim of this study was to prepare TSA self-soluble microneedles to strengthen its dermal retention and break through the difficulty of significantly thickening epidermal connective tissue and stratum corneum at the HS *** possible mechanism of action in suppressing HS was studied using human skin fibroblasts(HSF).Methods:Tanshinone Ⅱ_(A) self-dissolving microneedles(TSA-MN)was prepared using a negative mold casting *** prescription process of microneedle was optimized by Box-Behnken effect surface *** media were selected to investigate the ability of transdermal absorption and in vitro ***,according to Cell Counting Kit-8(CCK8)method as well as the Western blot method,the effect of TSA-MN on the biological characteristics of HSF was ***:With remarkable slow release effect and dermal retention,the release and transdermal properties of TSA-MN in vitro were better than both TSA and ordinary dosage *** effect of HSF confirmed the essential decrease in cell motility during cell proliferation and cell migration in vitro,which plays a significant role in down-regulating the secretion of transforming growth factor-β1(TGF-β1)in HSF and increasing the expression level of ***:The prepared TSA self-soluble microneedles is helpful in solving the problem of hypertrophic scars,with a stable dermal retention effect after process optimization.

关键词： human skin fibroblasts hypertrophic scar tanshinoneⅡ_(A) tanshinoneⅡ_(A)self-dissolving microneedles TGF-β1

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Mechanical performance and cyocompatibility of PU/PLCL nanofibrous electrospun scaffolds for skin regeneration

引用

Engineered Regeneration 2022年第1期3卷 53-58页

作者： Xiang Gao Meiling Wen Yang Liu Tian Hou Meiwen An Institute of Biomedical Engineering College of Biomedical EngineeringTaiyuan University of TechnologyShanxi Key Laboratory of Material Strength&Structural ImpactTaiyuanShanxi030024China Research Center for Nano-Biomaterials&Regenerative Medicine Department of Biomedical EngineeringCollege of Biomedical EngineeringTaiyuan University of TechnologyTaiyuan 030024China

skin tissue engineering with considerable skin regeneration capability is an urgent need for the wound *** current challenge for researchers is to develop a bionic scaffold that imitates the extracellular matrix for the regeneration of the damaged *** our study,poly(L-lactide-co-caprolactone)(PLCL)was blended with polyurethane(PU)to obtain nanofibrous scaffolds via *** electrospun fibers with 50%PLCL content had a certain number of intersections and jointing points,and exhibited significantly enhanced mechani-cal properties combined with suitable ***,cell activities demonstrated that PU/PLCL membranes had significantly biological advantages in enhanced growth of human skin fibroblasts with spreading morphology compared with PU membranes,indicating good cytocompatibility of composite *** findings proved that PU/PLCL electrospun membranes have great potential in applications of skin tissue engineering.

关键词： Electrospun membranes Poly(L-lactide-co-caprolactone) Polyurethane human skin fibroblasts skin tissue engineering

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mechanism and Clinical Observation of Effect of Growgx Yeast Fermentation Product Extract on Photo-aged HFF-1 Cells

引用

Medicinal Plant 2022年第1期13卷 26-29页

作者： Hong ZHANG Jianfei WAN Yuetong ZHAO Yang WU Mingli LEI Xiaoqun DUAN Pharmacy School Guilin Medical University Guilin 541004 China

[Objectives]To explore the effect of the extract of Growgx yeast fermentation product on ultraviolet A(UVA)-induced photo-aging of human skin fibroblasts(HFF-1)and its specific mechanisms from the level of cell biology,and discussed the application of the Growgx yeast fermentation product extract for skin aging.[Methods]A photo-aging model of HFF-1 cells was induced by UVA radiation meter,and it was given different concentrations of Growgx for *** proliferation activity of the HFF-1 cells was detected with MTT method,and their migration and invasion ability was measured by scratch *** SOD,HYP and MDA levels were detected with corresponding *** oxygen infusion apparatus assisted facial skin administration,the repair of the skin was observed through the VISIA skin tester.[Results]The survival rate of HFF-1 cells was reduced significantly by UVA with an irradiation dose of 10.8 J/cm2(Pskin spots and pores,making the skin smoother and more delicate.[Conclusions]Growgx can effectively fight against photo-aging caused by ultraviolet rays,and can significantly improve skin wrinkles.

关键词： Growgx yeast fermentation product extract human skin fibroblasts Anti-aging VISIA Clinical observation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The antioxidant Trolox restores mitochondrial membrane potential and Ca2+-stimulated ATP production in human complex Ⅰ deficiency

引用

世界最新医学信息文摘 2017年第30期 281-281页

作者： Felix Distelmaier Henk-Jan Visch Jan A.M.Smeitink Ertan Mayatepek Werner J.H.Koopman Peter H.G.M.Willems

Malfunction of mitochondrial complex I caused by nuclear gene mutations causes early-onset neurodegenerative *** work using cultured fibroblasts of complex-I-deficient patients revealed elevated levels of reactive oxygen species（ROS） and reductions in both total Ca2+content of the endoplasmic reticulum(ERCa) and bradykinin（Bk）-induced increases in cytosolic and mitochondrial free Ca2+([Ca2+]C;[Ca2+]M) and ATP（[ATP]C;[ATP]M） ***,we determined the mitochondrial membrane potential（Aw） in patient skin fibroblasts and show significant correlations with cellular ROS levels and ERCa,i.e.,the less negative Aw,the higher these levels and the lower *** with 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid（Trolox） normalized △Ψ and Bk-induced increases in[Ca2+]Mand[ATP]*** effects were accompanied by an increase in ERCaand Bkinduced increase in[Ca2+]***,these results provide evidence for an integral role of increased ROS levels in complex I deficiency and point to the potential therapeutic value of antioxidant treatment.

关键词： Reactive oxygen species Oxidative stress Mitochondrial membrane potential Endoplasmic reticulum Ca2+ content Mitochondrial disease Antioxidant human skin fibroblasts

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The antioxidant Trolox restores mitochondrial membrane potential and Ca2+-stimulated ATP production in human complex I deficiency

引用

世界最新医学信息文摘 2016年第11期 8-8页

作者： Felix Distelmaier Henk-Jan Visch Jan A.M.Smeitink Ertan Mayatepek Werner J.H.Koopman Peter H.G.M.Willems

Malfunction of mitochondrial complex I caused by nuclear gene mutations causes early-onset neurodegenerative diseases. Previous work using cultured fibroblasts of complex-I-deficient patients revealed elevated levels of reactive oxygen species（ros） and reductions in both total Ca2+content of the endoplasmic reticulum(ERCa) and bradykinin（Bk）-induced increases in cytosolic and mitochondrial free Ca2+([Ca2+]c; [Ca2+]M) and ATP（[ATP]C; [ATP]M） concentration. Here, we determined the mitochondrial membrane potential（Δψ） in patient skin fibroblasts and show significant correlations with cellular ros levels and ERCa, i.e., the less negative Δψ, the higher these levels and the lower ERCa. Treatment with 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid（Trolox） normalized Δψ and Bk-induced increases in [Ca2+]Mand [ATP]M. these effects were accompanied by an increase in ERCaand Bkinduced increase in [Ca2+]c. together, these results provide evidence for an integral role of increased ROS levels in complex I deficiency and point to the potential therapeutic value of antioxidant treatment.

关键词： Reactive oxygen species Oxidative stress Mitochondrial membrane potential Endoplasmic reticulum Ca2+ content Mitochondrial disease Antioxidant human skin fibroblasts

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：