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MicroRNAs of bone marrow mesenchymal stem cell-derived exosomes regulate acute myeloid leukemia cell proliferation and apoptosis

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Chinese Medical Journal 2020年第23期133卷 2829-2839页

作者： Yi-Chuan Xu Yan-Si Lin Ling Zhang Ying Lu Yan-Ling Sun Zhi-Gang Fang Zi-Yu Li Rui-Fang Fan Department of Hematology The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong 510630China Department of General practice Sun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhouGuangdong 510120China Department of Surgical Intensive Care Unit The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong 510630China Department of prevention and Health The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong 510630China

Background:Acute myeloid leukemia(AML)is a malignant hematological disease,originating from hematopoiesis stem cell differentiation obstruction and clonal *** reagents or biologicals for the treatment of AML are urgently needed,and exosomes have been identified as candidate biomarkers for disease diagnosis and *** study aimed to investigate the effects of exosomes from bone marrow mesenchymal stem cells(BMSCs)on AML cells as well as the underlying microRNA(mirNA)-mediated ***:Exosomes were isolated using a precipitation method,followed by validation using marker protein expression and nanoparticle tracking *** expressed mirNAs were identified by deep RNA sequencing and confirmed by quantitative real-time polymerase chain reaction(qpCR).Cell proliferation was assessed by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt method,and cell cycle progression and apoptosis were detected by flow *** gene expression was analyzed by qpCR and Western blotting(WB).Significant differences were determined using Student’s t test or analysis of ***:BMSCs-derived exosomes effectively suppressed cell proliferation(both pprogression(ppoptosis(ppressed mirNAs obtained from BMSCs-derived exosomes compared with KG-1a cell-derived exosomes(p5).Knockdown of hsa-mir-124-5p in BMSCs abrogated the effects of BMSCs-derived exosomes in regulating KG-1a such as the change in cell proliferation(both pppressed expression of structural maintenance of chromosomes 4(ppCR and pprogression of various *** BMSCs-derived exosomes effect

关键词： Exosomes Mesenchymal stem cell Acute myeloid leukemia hsa-mir-124-5p

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